Lectin-resistant CHO glycosylation mutants
Abstract
Chinese hamster ovary (CHO) mutant cells with a wide variety of alterations in the glycosylation of proteins and lipids have been isolated by selection for resistance to the cytotoxicity of plant lectins. These CHO mutants have been used to characterize glycosylation pathways, to identify genes that code for glycosylation activities, to elucidate functional roles of glycans that mediate biological processes, and for glycosylation engineering. In this chapter, we briefly describe the available panel of lectin-resistant CHO mutants and summarize their glycan alterations and the biochemical and genetic bases of mutation.
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CHO cells are unable to produce some types of human glycosylation, such as α-2,6-sialylation and α-1,3/4-fucosylation.
“Although CHO cells possess many advantages for glycoprotein productions, they are unable to produce some types of human glycosylation, such as α-2,6-sialylation and α-1,3/4-fucosylation (Patnaik and Stanley, 2006).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Patnaik and Stanley, 2006
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- Authors
- Patnaik, S. K., & Stanley, P.
- Affiliations
- Albert Einstein College of Medicine (US)
- Venue
- Methods in Enzymology
- Publisher
- Elsevier
- Published
- 2006
- Volume
- 416
- Pages
- 159-182
- ISSN
- 0076-6879 (ISSN-L)
- OpenAlex
- W2105081087
- Cited by
- 218
- References
- 1
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 14 · 2023: 6 · 2022: 11 · 2021: 10 · 2020: 10 · 2019: 10 · 2018: 12 · 2017: 20 · 2016: 9 · 2015: 9 · 2014: 19 · 2013: 12 · 2012: 18
- FWCI
- 13.31
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Methods in enzymology on CD-ROM/Methods in enzymology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Public Health Service (R0136434)
- National Cancer Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Physical Sciences › Chemistry › Organic Chemistry › Carbohydrate Chemistry and Synthesis
- Keywords
- CHO cells, protein glycosylation, glycan function, glycosylation engineering, mutant selection
- MeSH terms
- Animals, Cell Line, Cricetulus, Glycosylation, Cricetinae, Mutation, Polysaccharides, CHO Cells, Lectins
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