Cited by 258Autophagy in Disease and TherapyAdvanced Electron Microscopy Techniques and ApplicationsCellular transport and secretion

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Chapter 10 Monitoring Autophagy by Electron Microscopy in Mammalian Cells

Ylä‐Anttila, Päivi, Vihinen, Helena, Jokitalo, Eija, Eskelinen, Eeva‐Liisa·2009·Methods in Enzymology

DOI 10.1016/s0076-6879(08)03610-0

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Abstract

Electron microscopy remains one of the most accurate methods for the detection of autophagy and quantification of autophagic accumulation. Compared to fluorescence microscopy, the resolution of transmission electron microscopy is superior. In this chapter we describe the fine structure of early and late autophagic compartments in mammalian cells. Instructions are given for the preparation of samples for conventional electron microscopy using three different protocols suitable for cultured cells and animal tissues. We also introduce tomography as a tool to study the three-dimensional morphology of autophagic organelles and show the morphology of a phagophore as an example. Finally, we describe a protocol for the quantification of autophagic compartments by electron microscopy and point counting.

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LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.
Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y., & Yoshimori, T. (2000) · The EMBO Journal · doi:10.1093/emboj/19.21.5720 · Cited by 6,605
Autophagosome formation: core machinery and adaptations
Xie, Z., & Klionsky, D. J. (2007) · Nature Cell Biology · doi:10.1038/ncb1007-1102 · Cited by 2,237
Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
Klionsky, D. J., Abeliovich, H., Agostinis, P., Agrawal, D. K., Aliev, G., Askew, D. S., Baba, M., Baehrecke, E. H., Bahr, B. A., Ballabio, A., et al. (2008) · Autophagy · doi:10.4161/auto.5338 · Cited by 2,230
Principles and Techniques of Electron Microscopy: Biological Applications
Maugel, T. K., & Hayat, M. A. (1979) · Transactions of the American Microscopical Society · doi:10.2307/3226038 · Cited by 1,327
A Unified Nomenclature for Yeast Autophagy-Related Genes
Klionsky, D. J., Cregg, J. M., Dunn, W. A., Emr, S. D., Sakai, Y., Sandoval, I. V., Sibirny, A. А., Subramani, S. E., Thumm, M., Marten Veenhuis, A., et al. (2003) · Developmental Cell · doi:10.1016/s1534-5807(03)00296-x · Cited by 1,305
Unbiased Stereology: Three-Dimensional Measurement in Microscopy
Howard, C. V., & Reed, M. (1998) · Cited by 1,149
Role for Rab7 in maturation of late autophagic vacuoles
Jäger, S., Bucci, C., Tanida, I., Ueno, T., Kominami, E., Säftig, P., & Eskelinen, E. (2004) · Journal of Cell Science · doi:10.1242/jcs.01370 · Cited by 970
Unbiased Stereology. Three‐Dimensional Measurement in Microscopy.
Sandau, K. (1999) · Journal of Microscopy · doi:10.1046/j.1365-2818.1999.0429a.x · Cited by 890
Studies on the mechanisms of autophagy: formation of the autophagic vacuole.
Dunn, W. A. (1990) · The Journal of Cell Biology · doi:10.1083/jcb.110.6.1923 · Cited by 787
Maturation of Autophagic Vacuoles in Mammalian Cells
Eskelinen, E.-L. (2005) · Autophagy · doi:10.4161/auto.1.1.1270 · Cited by 626

Show all 26 references

Research works printed but not linked: 1

Eskelinen, 2008, Fine structure of the autophagosome, Methods Mol. Biol., 445, 11, 10.1007/978-1-59745-157-4_2 · DOI

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Items 201–258 of 258

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“Generally, the initial autophagic vacuoles, also called autophagosomes, typically had a double membrane [55], whereas there was typically only one limiting membrane in the AVd, and it usually contained electron-dense cytoplasmic and/or organelles at various stages of degradation [56, 57].”
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Gouda, K. M., AbdelHamid, S. G., Mansour, A., Omar, N. N., & EL-Mesallamy, H. O. (2021) · Current Issues in Molecular Biology · doi:10.3390/cimb43030120 · Cited by 6
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[Analysis of blood cell autophagy distribution in hematologic diseases by transmission electron microscope].
Dong, S., Zhao, S., Wang, Y., Pang, T., & Ru, Y. (2015) · PubMed Central · doi:10.3760/cma.j.issn.0253-2727.2015.02.013 · Cited by 6
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Hao, Y., Duan, F., Dong, X., Bi, R., Wang, Y., Zhu, S., & Hu, J. (2025) · Analytical Cellular Pathology · doi:10.1155/ancp/6648632 · Cited by 5
“The methods for counting AVs (autophagosomes and autolysosomes) were as described previously [19].”
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Keary, K. M., Gu, Q.-H., Chen, J., & Li, Z. (2022) · Cell Reports · doi:10.1016/j.celrep.2023.112898 · Cited by 6
“30,31 To ensure that only autophagosomes in CA1 pyramidal neurons were assessed, a custom Fiji script was used to measure RFP puncta colocalized with eYFP.”
Pellino 3 E3 ligase promotes starvation-induced autophagy to prevent hepatic steatosis
Kolapalli, S. P., Beese, C. J., Reid, S. E., Brynjólfsdóttir, S. H., Jørgensen, M. H., Jain, A., Cuenco, J., Lewińska, M., Abdul-Al, A., López, A. R., et al. (2025) · Science Advances · doi:10.1126/sciadv.adr2450 · Cited by 5
Acute exposure to thimerosal induces antiproliferative properties, apoptosis, and autophagy activation in human Chang conjunctival cells
Zhang, H., Wu, H., Wang, C., Xie, J., He, J., Yang, J. J., & Ye, J. (2014) · Graefe s Archive for Clinical and Experimental Ophthalmology · doi:10.1007/s00417-013-2542-x · Cited by 5
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Sakurai, H. T., Arakawa, S., Noguchi, S., & Shimizu, S. (2022) · Scientific Reports · doi:10.1038/s41598-022-26430-5 · Cited by 4
Methods to Measure Autophagy in Cancer Metabolism
von Muhlinen, N. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9027-6_10 · Cited by 4
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Biazik, J. M., Vihinen, H., Jokitalo, E., & Eskelinen, E. (2016) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2016.09.063 · Cited by 4
UBE2D2 promotes gastric cancer progression by inhibiting ferroptosis through autophagy-dependent stabilization of CST1
Yang, Y., Xu, J., Zhao, Q., Zhang, R., Wang, Y., Shang, C., Pan, S., Hu, C., & Luo, C. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.147333 · Cited by 3
ARRB1 inhibits extracellular matrix degradation and apoptosis of nucleus pulposus cells by promoting autophagy and attenuates intervertebral disc degeneration
Dan, X., Gu, X., Zi, Y., Xu, J., Wang, C., Li, C., Hu, X., Wu, Z., Yu, Y., & Ma, B. (2024) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2024.119769 · Cited by 3
Autophagic activity in the mouse urinary bladder urothelium as a response to starvation
Erman, A., Resnik, N., & Romih, R. (2012) · PROTOPLASMA · doi:10.1007/s00709-012-0387-5 · Cited by 3
Total ginsenoside and Acorus tatarinowii volatile oil co-administration reverses learning memory deficits, reduces Aβ42 and AChE levels, and downregulates the PI3K/Akt/mTOR/Beclin-1 autophagic pathway in APP/PS1 double transgenic mice
Deng, M., Zhong, X., Ning, B., Zhang, Q., Fang, Y., & Huang, L. (2022) · Pharmacological Research - Modern Chinese Medicine · doi:10.1016/j.prmcm.2022.100043 · Cited by 3
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Specific localization of LC3B in autophagosome: A correlative labelling study with nanoparticle in oral squamous cell carcinoma
Lai, K., Killingsworth, M. C., Yong, J. L. C., Matthews, S. T., Ebrahimi, A., McGuinness, J., Ngo, Q. D., Caixeiro, N., Hong, A., & Lee, C. S. (2017) · Experimental and Molecular Pathology · doi:10.1016/j.yexmp.2017.05.007 · Cited by 3
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Zhou, F.-L., Li, S. C., Zhu, Y., Guo, W.-J., Shao, L.-J., Nelson, J., Simpkins, S. W., Yang, D., Liu, Q., Yashiroda, Y., et al. (2019) · Acta Pharmacologica Sinica · doi:10.1038/s41401-019-0231-y · Cited by 3
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[Autophagy and its relationship with tumor proliferation, invasion, and treatment].
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Onwah, S. S., Uzonna, J. E., & Ghavami, S. (2024) · Methods in molecular biology · doi:10.1007/7651_2024_517 · Cited by 1
Methods for Measuring Autophagy Levels in Disease
Phadwal, K., & Kurian, D. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-805420-8.00009-3 · Cited by 1
AUTOPHAGY; Recent Advances in Health and Disease
Elfadl, S. A. (2018) · Journal of Medical Histology/Journal of Medical Histology · doi:10.21608/jmh.2018.5225.1040 · Cited by 1
Assessment of autophagy in Leishmania parasitesPreprint
Onwah, S. S., Uzonna, J. E., & Ghavami, S. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.01.03.574013 · Cited by 1
Based on Network Pharmacology and Experimental Validation: β-Sitosterol Inhibits Cytokine Storm by Inducing AutophagyPreprint
Li, Y., Xu, J., Li, H., Xiao, X., Yang, X., Cheng, Y., & Huang, Y. (2024) · Research Square · doi:10.21203/rs.3.rs-4627381/v1 · Cited by 1
Autophagy's Contribution to Innate and Adaptive Immunity: An Overview
Bell, C., Desjardins, M., Thibault, P., & Radtke, K. (2014) · Wiley eBooks · doi:10.1002/9781118677551.ch16
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6-phosphofructo-2-kinase inhibition induces autophagy as a survival mechanism.
Klarer, A. C. (2013) · ThinkIR: The University of Louisville's Institutional Repository (University of Louisville) · doi:10.18297/etd/760
“An increased number of intracellular structures including double-membrane bound vesicles, consistent with autophagosomes were visualized in cells transfected with PFKFB3 siRNA [111].”
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Zhong, X. Y. (2014) · University of Minnesota Digital Conservancy (University of Minnesota)
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Cyr, D. P. (2013)
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Biazik, J. M., Vihinen, H., Jokitalo, E., & Eskelinen, E. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-801043-3.00002-9
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Steele, S. P. (2019) · Carolina Digital Repository (University of North Carolina at Chapel Hill) · doi:10.17615/b2hs-hz24
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Noor, S. (2011) · TSpace (University of Toronto)
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Honkanen, R. (2016) · Työväentutkimus Vuosikirja
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Perrotta, I. D. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-805253-2.00014-6
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Ong, S. (2014) · Lecture notes in bioengineering · doi:10.1007/978-981-4585-72-9_5 · Cited by 1
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Abdel-Mohsen, M. A., Badawy, A. M., Abu-Youssef, M. A. M., Yehia, M. A., Shamaa, L. D. A., & Abdullah, S. A. (2023) · Research Square · doi:10.21203/rs.3.rs-2451740/v1
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Kreft, M. E., Mironov, A., Beznoussenko, G. V., Tratnjek, L., Morelli, E., Ishihara, N., Mihara, K., Ossolengo, G., Barozzi, S., Parazzoli, D., et al. (2025) · Research Square · doi:10.21203/rs.3.rs-6857499/v1
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Biggers, J. (2010) · VCU Scholars Compass (Virginia Commonwealth University) · doi:10.25772/xs7a-b628
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Testi, S. (2018) · doi:10.70675/8a7bf4d1z207ez43dbza1a2z8ed747cc61e3
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Zagkou, S. (2021) · theses.fr (ABES) · doi:10.70675/945352cczdaf3z45fczb8dezdc79d3ca5100
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Silva, F. R., Dias, P. R. F., Pavan, I. C. B., de Oliveira, A. P., Basei, F. L., dos Santos, L. E., de Sousa, L. M., Consonni, S. R., de Oliveira, A. G., Silveira, L. R., et al. (2026) · Cells · doi:10.3390/cells15100924
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Gorin, J. (2013)

Authors: Ylä‐Anttila, P., Vihinen, H., Jokitalo, E., & Eskelinen, E.
Affiliations: University of Helsinki (FI)
ORCID: Ylä‐Anttila, Päivi
Vihinen, Helena
Jokitalo, Eija
Eskelinen, Eeva‐Liisa
Venue: Methods in Enzymology
Publisher: Elsevier
Published: 2009
Volume: 452
Pages: 143-164
ISSN: 0076-6879 (ISSN-L)
DOI: 10.1016/s0076-6879(08)03610-0
OpenAlex: W1594293927
Cited by: 257
References: 26
Citations by year: 2026: 4 · 2025: 7 · 2024: 13 · 2023: 6 · 2022: 14 · 2021: 14 · 2020: 19 · 2019: 27 · 2018: 17 · 2017: 25 · 2016: 19 · 2015: 18 · 2014: 20 · 2013: 17 · 2012: 19
FWCI: 37.92
Type: book chapter
Language: en
Open access: closed
Locations: Methods in enzymology on CD-ROM/Methods in enzymology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords: autophagy, phagophore, electron microscopy specimen preparation, electron tomography, three-dimensional morphology, point counts, mammalian cells
MeSH terms: Animals, Autophagy, Cells, Cultured, Microscopy, Electron, Transmission, Mice

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Chapter 10 Monitoring Autophagy by Electron Microscopy in Mammalian Cells

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