Measuring Mitochondrial Protein Thiol Redox State
Abstract
Protein thiols are an important component of mammalian intramitochondrial antioxidant defenses owing to their selective interaction with reactive oxygen and nitrogen species (ROS and RNS). Reversible modifications of protein thiols resulting from these interactions are also an important aspect of redox signal transduction. Therefore, to assess how mitochondria respond to oxidative stress and act as nodes in redox signaling pathways, it is important to measure general changes to protein thiol redox states and also to identify the specific mitochondrial thiol proteins involved. Here we outline some of the approaches that can be used to accomplish these goals and thereby infer the multiple roles of mammalian mitochondrial protein thiols in antioxidant defense and redox signaling.
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The loss of ND3 S-nitrosation in vivo is due to reduction by mitochondrial glutathione and thioredoxin thiol reductants.
“suggesting that reduction in vivo occurs by thiol reductants of the mitochondrial glutathione and thioredoxin systems (Supplementary Fig. 12)30.”
From Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I (2013) · cites this paper as [30]
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- Authors
- Requejo, R., Chouchani, E. T., Hurd, T. R., Menger, K. E., Hampton, M. B., & Murphy, M. P.
- Affiliations
- MRC Mitochondrial Biology Unit (GB)
- University of Otago (NZ)
- Venue
- Methods in Enzymology
- Publisher
- Elsevier
- Published
- 2010
- Volume
- 474
- Pages
- 123-147
- ISSN
- 0076-6879 (ISSN-L)
- OpenAlex
- W137962783
- Cited by
- 31
- References
- 57
- Citations by year
- 2023: 1 · 2021: 5 · 2019: 3 · 2018: 4 · 2017: 2 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 3 · 2012: 2
- FWCI
- 3.87
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Methods in enzymology on CD-ROM/Methods in enzymology (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
- Keywords
- thiol redox state, mitochondrial antioxidant defense, reactive oxygen species, reactive nitrogen species, redox signaling, oxidative stress, thiol modification
- MeSH terms
- Glutathione, Liver, Oxidation-Reduction, Protein Binding, Sulfhydryl Compounds, Blotting, Western, Signal Transduction, Mitochondrial Proteins, Myocytes, Cardiac
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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