Cited by 1,365Alzheimer's disease research and treatmentsPrion Diseases and Protein MisfoldingAmyloidosis: Diagnosis, Treatment, Outcomes

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[18] Quantification of β-sheet amyloid fibril structures with thioflavin T

LeVine, Harry·1999·Methods in Enzymology

DOI 10.1016/s0076-6879(99)09020-5

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BackgroundMethod
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“Inhibition of Aβ1-42 Aggregation The Aβ1-42 self-aggregation process was studied using the thioflavin-T assay [62,63].”
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“The ThT binding assay was performed according to LeVine [59], using a 25 µM ThT solution in 20 mM sodium phosphate buffer, pH 7.0.”
MethodInfluential
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Grimaldi, A., Tettamanti, G., Congiu, T., Girardello, R., Malagoli, D., Falabella, P., Valvassori, R., Ottaviani, E., & de Eguileor, M. (2012) · Cell and Tissue Research · doi:10.1007/s00441-012-1503-8 · Cited by 16
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“Thioflavin T fluorescence has been used to trace the kinetics of fibrillation (Le Vine 1999).”
Method
Glyoxal induced glycative insult suffered by immunoglobulin G and fibrinogen proteins: A comparative physicochemical characterization to reveal structural perturbations
Alouffi, S., Shahab, U., Khan, S. A., Khan, M., Khanam, A., Akasha, R., Shahanawaz, S., Arif, H., Tahir, I. K., Rehman, S., et al. (2022) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2022.02.093 · Cited by 15
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Advanced analytical methodologies in Alzheimer’s disease drug discovery
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Amyloid-like Behavior of Site-Specifically Citrullinated Myelin Oligodendrocyte Protein (MOG) Peptide Fragments inside EBV-Infected B-Cells Influences Their Cytotoxicity and Autoimmunogenicity
Araman, C., van Gent, M. E., Meeuwenoord, N. J., Heijmans, N., Marqvorsen, M. H. S., Doelman, W., Faber, B. W., Hart, B. A. ‘., & van Kasteren, S. I. (2018) · Biochemistry · doi:10.1021/acs.biochem.8b00852 · Cited by 16
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Recombinant-phospholipase A2 production and architecture of inclusion bodies are affected by pH in Escherichia coli
Calcines-Cruz, C., Olvera, A., Castro‐Acosta, R. M., Zavala, G., Alagon, A. C., Trujillo‐Roldán, M. A., & Valdez‐Cruz, N. A. (2017) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2017.10.178 · Cited by 19
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“To elucidate the possibility of existence of cross-β-structure in the PVA virions and the free PVA CP, we applied the ThT-fluorescence test widely used in recent years to assess the presence of amyloid-like structures in proteins (LeVine, 1999; Nilsson, 2004).”
BackgroundMethod
Engineering of a peptide probe for β-amyloid aggregates
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Enhancement and Induction of HIV‐1 Infection through an Assembled Peptide Derived from the CD4 Binding Site of gp120
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β-Hairpin Peptide Mimics Decrease Human Islet Amyloid Polypeptide (hIAPP) Aggregation
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“ThT is a dye able to fluoresce upon binding to β-sheet rich structures (LeVine, 1999) and it is the standard dye used to study the aggregation kinetics of amyloidogenic proteins (Gade Malmos et al., 2017).”
Background
Raman spectral imaging of 13C2H15N-labeled α-synuclein amyloid fibrils in cells
Watson, M., Flynn, J. D., & Lee, J. C. (2020) · Biophysical Chemistry · doi:10.1016/j.bpc.2020.106528 · Cited by 14
Peroxynitrite scavenger FeTPPS effectively inhibits hIAPP aggregation and protects against amyloid induced cytotoxicity.
Zhang, P., Zeng, L., Gao, W.-X., Li, H.-L., & Gao, Z.-H. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.06.034 · Cited by 14
Avenanthramide-C prevents amyloid formation of bovine serum albumin
Vázquez, M. G. Q., Montiel-Condado, D., Hernández, B. G., & González-Horta, A. (2020) · Biophysical Chemistry · doi:10.1016/j.bpc.2020.106391 · Cited by 18
Heme prevents highly amyloidogenic human calcitonin (hCT) aggregation: A potential new strategy for the clinical reuse of hCT
Ye, H., Zhou, J., Li, H., & Gao, Z. (2019) · Journal of Inorganic Biochemistry · doi:10.1016/j.jinorgbio.2019.03.026 · Cited by 17
Basified Human Lysozyme: A Potent Inhibitor against Amyloid β-Protein Fibrillogenesis
Li, X., Xie, B., & Sun, Y. P. (2018) · Langmuir · doi:10.1021/acs.langmuir.8b03278 · Cited by 15
Amyloids of multiple species: are they helpful in survival?
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“Amyloids binds with certain dyes such as Congo Red (CR) and thioflavin T (ThT) (Klunk, Jacob & Mason, 1999; LeVine, 1999).”
Background
Supratherapeutic concentrations of cilostazol inhibits β-amyloid oligomerization in vitro
Shozawa, H., Oguchi, T., Tsuji, M., Yano, S., Kiuchi, Y., & Ono, K. (2018) · Neuroscience Letters · doi:10.1016/j.neulet.2018.04.032 · Cited by 15
Newfound effect of N-acetylaspartate in preventing and reversing aggregation of amyloid-beta in vitro
Dollé, J.-P., Rodgers, J. M., Browne, K. D., Troxler, T., Gai, F., & Smith, D. H. (2018) · Neurobiology of Disease · doi:10.1016/j.nbd.2018.05.023 · Cited by 15
“When ThT binds to β-sheets, a strong fluorescence signal is emitted allowing for the kinetics of β-sheet formation to be monitored over time (LeVine, 1999).”
Synthesis of chemically-tethered amyloid-β segment trimer possessing amyloidogenic properties.
Shinoda, K., Sohma, Y., & Kanai, M. (2015) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2015.05.029 · Cited by 14
In Vitro Amyloidogenic Peptides of Galectin-7
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“Amyloid fibril formation was measured with the ThT method (35, 36).”
Method
Investigation of the kinetics of insulin amyloid fibrils formation
Sulatskaya, A. I., Волова, Е. А., Komissarchik, Y. Y., Snigirevskaya, E. S., Maskevich, A. A., Drobchenko, E. A., Kuznetsova, I. М., & Константинович, Т. К. (2014) · Cell and Tissue Biology · doi:10.1134/s1990519x14020114 · Cited by 14

Authors: LeVine, H.
Corresponding author: LeVine, Harry
Venue: Methods in Enzymology
Publisher: Elsevier
Published: 1999
Volume: 309
Pages: 274-284
ISSN: 0076-6879 (ISSN-L)
DOI: 10.1016/s0076-6879(99)09020-5
OpenAlex: W1588296570
Cited by: 1,365
References: 25
Citations by year: 2026: 26 · 2025: 34 · 2024: 56 · 2023: 42 · 2022: 51 · 2021: 54 · 2020: 60 · 2019: 78 · 2018: 86 · 2017: 69 · 2016: 61 · 2015: 67 · 2014: 78 · 2013: 58 · 2012: 66
FWCI: 28.63
Type: book chapter
Language: en
Open access: closed
Locations: Methods in enzymology on CD-ROM/Methods in enzymology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Prion Diseases and Protein Misfolding
Health Sciences › Medicine › Internal Medicine › Amyloidosis: Diagnosis, Treatment, Outcomes
Keywords: thioflavin T, amyloid fibrils, beta-sheet, amyloid quantification, Congo red, amyloid staining, amyloid fibril formation, fluorescence spectroscopy, Congo red birefringence
MeSH terms: Fluorescence, Hydrogen-Ion Concentration, Protein Binding, Spectrum Analysis, Thiazoles, Amyloid beta-Peptides, Protein Structure, Secondary, Benzothiazoles

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[18] Quantification of β-sheet amyloid fibril structures with thioflavin T

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