Cited by 1,365Alzheimer's disease research and treatmentsPrion Diseases and Protein MisfoldingAmyloidosis: Diagnosis, Treatment, Outcomes

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[18] Quantification of β-sheet amyloid fibril structures with thioflavin T

LeVine, Harry·1999·Methods in Enzymology

DOI 10.1016/s0076-6879(99)09020-5

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“Quantification of the ThT signal is typically performed in monoprotein experiments, i.e., to monitor fibrillation of protein solutions (LeVine, 1999; Morel et al., 2010) or decay of amyloids fibrils (Morales-Belpaire and Gerin, 2008), and rarely in biofilms of pure cultures of micro-organisms…”
Background
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“Each construct was shaken at 37 C for 138 h with ThT, a dye that fluoresces in the presence of amyloid fibers (28, 29).”
Method
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Background
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Background
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Background
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“and Abeta-peptide (1-42) amyloid fibrils, however, smaller than that for ThT bound to the another mode (with higher affinity) of amyloid fibrils (Table 1).”
Background
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Retracted
Inhibition of Alzheimer's Aβ1‐42 Fibrillogenesis and Removal of Copper Ions by Polypeptides Modified Gold Nanoparticles
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“Fluorescent molecules such as 4-(5-methoxythiazolo[4,5-b]pyridin-2-yl)benzoic acid, ThT and Congo red can be used to detect the formation of amyloid fibrils.([44,45,46]) Due to the special bonding properties of ThT to amyloid peptide, the fluorescence of ThT is widely used in the study of amyloid fibril formation.”
BackgroundMethod
Design, synthesis and structure-activity relationship studies of 3-phenylpyrazino[1,2-a]indol-1(2H)-ones as amyloid aggregation and cholinesterase inhibitors with antioxidant activity
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“ThT could bind to β-sheet structures in amyloid fibrils specifically and ThT assay is regarded as the gold standard to explore the formation of amyloid fibrils, where the intensity of ThT could reflect the quantification of amyloid fibrils (LeVine 1999; Bleem et al.”
Background
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“We first examined the effect of Fe-TMPyP on the formation of mature amyloid fibrils, using the fluorescence from ThT, a dye whose absorption and emission spectra change upon binding to amyloid.(59) ThT fluorescence was measured for samples incubated in a 96-well microplate under standard aggregation conditions (see Ma…”
Method
Membrane-Modulating Drugs can Affect the Size of Amyloid-β25–35 Aggregates in Anionic Membranes
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Tryptophan Probes of TDP-43 C-Terminal Domain Amyloid Formation
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“The increase of magnitude in ThT fluorescence intensity makes it an unusually sensitive and efficient reporter as roughly related to the amount of fibrillary structure in solution (Biancalana & Koide, 2010; LeVine, 1999).”
Background
Tannic Acid-Induced Surface-Catalyzed Secondary Nucleation during the Amyloid Fibrillation of Hen Egg-White Lysozyme
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“ThT is a dye commonly used in the diagnostics of amyloid fibrils [6].”
Assays for Light Chain Amyloidosis Formation and Cytotoxicity
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Authors: LeVine, H.
Corresponding author: LeVine, Harry
Venue: Methods in Enzymology
Publisher: Elsevier
Published: 1999
Volume: 309
Pages: 274-284
ISSN: 0076-6879 (ISSN-L)
DOI: 10.1016/s0076-6879(99)09020-5
OpenAlex: W1588296570
Cited by: 1,365
References: 25
Citations by year: 2026: 26 · 2025: 34 · 2024: 56 · 2023: 42 · 2022: 51 · 2021: 54 · 2020: 60 · 2019: 78 · 2018: 86 · 2017: 69 · 2016: 61 · 2015: 67 · 2014: 78 · 2013: 58 · 2012: 66
FWCI: 28.63
Type: book chapter
Language: en
Open access: closed
Locations: Methods in enzymology on CD-ROM/Methods in enzymology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Prion Diseases and Protein Misfolding
Health Sciences › Medicine › Internal Medicine › Amyloidosis: Diagnosis, Treatment, Outcomes
Keywords: thioflavin T, amyloid fibrils, beta-sheet, amyloid quantification, Congo red, amyloid staining, amyloid fibril formation, fluorescence spectroscopy, Congo red birefringence
MeSH terms: Fluorescence, Hydrogen-Ion Concentration, Protein Binding, Spectrum Analysis, Thiazoles, Amyloid beta-Peptides, Protein Structure, Secondary, Benzothiazoles

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[18] Quantification of β-sheet amyloid fibril structures with thioflavin T

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