Cited by 1,000RNA and protein synthesis mechanismsRNA modifications and cancerEnzyme Structure and Function

Structure of Functionally Activated Small Ribosomal Subunit at 3.3 Å Resolution

Open access
Schluenzen, Frank, Tocilj, Ante, Zarivach, Raz, Harms, Joerg, Gluehmann, Marco, Janell, Daniela, Bashan, Anat, Bartels, Heike +3 more·2000·Cell

Abstract

The small ribosomal subunit performs the decoding of genetic information during translation. The structure of that from Thermus thermophilus shows that the decoding center, which positions mRNA and three tRNAs, is constructed entirely of RNA. The entrance to the mRNA channel will encircle the message when a latch-like contact closes and contributes to processivity and fidelity. Extended RNA helical elements that run longitudinally through the body transmit structural changes, correlating events at the particle's far end with the cycle of mRNA translocation at the decoding region. 96% of the nucleotides were traced and the main fold of all proteins was determined. The latter are either peripheral or appear to serve as linkers. Some may assist the directionality of translocation.

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Crossref · 10.1016/s0092-8674(00)00084-2

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71 of 71 references verified0 citation errors · 0 not checkable

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  • These changes could be mediated by S12, which is in close proximity to the top of h44 and also appears to interact with Tet(O).

    “These changes could be mediated by S12, which is in close proximity to the top of h44 (43, 58) and also appears to interact with Tet(O) (45).”

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Authors
Schluenzen, F., Tocilj, A., Zarivach, R., Harms, J., Gluehmann, M., Janell, D., Bashan, A., Bartels, H., Agmon, I., Franceschi, F., & Yonath, A.
Affiliations
Weizmann Institute of Science (IL)
Venue
Cell
Publisher
Elsevier BV
Published
2000-09
Volume
102
Issue
5
Pages
615-623
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2092617311
Cited by
979
References
72
Citations by year
2026: 11 · 2025: 13 · 2024: 18 · 2023: 28 · 2022: 18 · 2021: 26 · 2020: 27 · 2019: 18 · 2018: 22 · 2017: 25 · 2016: 18 · 2015: 31 · 2014: 26 · 2013: 27 · 2012: 43
FWCI
28.84
Type
journal article
Language
en
Open access
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (GM-34360, R01GM034360)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
Keywords
small ribosomal subunit, ribosome structure, Thermus thermophilus, decoding center, tRNA binding, mRNA translocation, translation fidelity, ribosomal proteins
MeSH terms
Binding Sites, Models, Molecular, Nucleic Acid Conformation, Protein Conformation, Ribosomes, RNA, Bacterial, RNA, Messenger, RNA, Ribosomal, 16S, RNA, Transfer, Structure-Activity Relationship, Thermus thermophilus, Crystallography, X-Ray, Base Pairing

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