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The amnesiac Gene Product Is Expressed in Two Neurons in the Drosophila Brain that Are Critical for Memory

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Waddell, Scott, Armstrong, J.Douglas, Kitamoto, Toshihiro, Kaiser, Kim, Quinn, William G.·2000·Cell

DOI 10.1016/s0092-8674(00)00183-5

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Abstract

Mutations in the amnesiac gene in Drosophila affect both memory retention and ethanol sensitivity. The predicted amnesiac gene product, AMN, is an apparent preproneuropeptide, and previous studies suggest that it stimulates cAMP synthesis. Here we show that, unlike other learning-related Drosophila proteins, AMN is not preferentially expressed in mushroom bodies. Instead, it is strongly expressed in two large neurons that project over all the lobes of the mushroom bodies, a finding that suggests a modulatory role for AMN in memory formation. Genetically engineered blockade of vesicle recycling in these cells abbreviates memory as in the amnesiac mutant. Moreover, restoration of amn gene expression to these cells reestablishes normal olfactory memory in an amn deletion background. These results indicate that AMN neuropeptide release onto the mushroom bodies is critical for normal olfactory memory.

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Authors: Waddell, S., Armstrong, J. D., Kitamoto, T., Kaiser, K., & Quinn, W. G.
Affiliations: Massachusetts Institute of Technology (US)
University of Glasgow (GB)
Beckman Research Institute (US)
Corresponding author: Waddell, Scott
ORCID: Waddell, Scott
Armstrong, J.Douglas
Kitamoto, Toshihiro
Quinn, William G.
Venue: Cell
Publisher: Elsevier BV
Published: 2000-11
Volume: 103
Issue: 5
Pages: 805-813
ISSN: 0092-8674 (ISSN-L)
DOI: 10.1016/s0092-8674(00)00183-5
OpenAlex: W2140882620
Cited by: 332
References: 54
Citations by year: 2026: 8 · 2025: 12 · 2024: 12 · 2023: 13 · 2022: 7 · 2021: 7 · 2020: 9 · 2019: 5 · 2018: 13 · 2017: 14 · 2016: 8 · 2015: 13 · 2014: 7 · 2013: 14 · 2012: 12
FWCI: 6.65
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 4641 d)
Locations: Cell (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords: olfactory memory, mushroom body, vesicle recycling, neuropeptide release, memory retention, ethanol sensitivity, cAMP signaling, memory formation
MeSH terms: Cyclic AMP, Animals, Brain, Chromosomes, Drosophila, Genotype, Immunohistochemistry, Memory, Models, Biological, Mutation, Neurons, Neuropeptides, Peptides, Phenotype, Smell, Temperature, Time Factors, Gene Deletion, Genes, Reporter, Drosophila Proteins, Animals, Genetically Modified

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The amnesiac Gene Product Is Expressed in Two Neurons in the Drosophila Brain that Are Critical for Memory

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