Cited by 333Neuroscience and Neuropharmacology ResearchMemory and Neural MechanismsNeurotransmitter Receptor Influence on Behavior

Dopaminergic antagonists prevent long-term maintenance of posttetanic LTP in the CA1 region of rat hippocampal slices

Frey, Uwe, Schroeder, Helmut, Matthies, Hansju¨rgen·1990·Brain Research

Abstract

The involvement of dopaminergic mechanisms in the induction and maintenance of posttetanic long-term potentiation (LTP) was investigated on CA1 cells of rat hippocampal slices. The presence of the dopamine receptor blocker domperidone in a concentration of 1 microM during tetanization with 3 trains of 100 impulses (100 Hz) and a train interval of 10 min influences neither the synaptic transmission nor the induction of LTP. However, the potentiation of both the population spike and the population EPSP gradually decreases, thus significantly differing from control LTP about 4 h after initiation and reaching the level of non-tetanized controls about 7-8 h after tetanization. The simultaneous presence of 1 microM apomorphine during tetanization abolishes this effect of domperidone indicating the specific dopaminolytic nature of its action. Also the presence of the dopamine antagonists sulpiride and flupenthixol, respectively, in a concentration of 1 microM during tetanization likewise prevents the occurrence of the late LTP maintenance. The determination of [14C]dopamine in 2 min fractions from the superfused slices after preloading during a preincubation period revealed that a low frequency stimulation of the Schaffer collaterals with 0.33 Hz does not influence the spontaneous efflux of dopamine, whereas the tetanization with an impulse train of 100 Hz produces a significantly enhanced release. The observations suggest that dopaminergic influences during and immediately after tetanization at least additionally contribute to the induction of postsynaptic mechanisms subserving a late, long-lasting maintenance of potentiation. The results also support the assumed existence of different subsequent stages of LTP.

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Crossref · 10.1016/0006-8993(90)91578-5

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  • The persistence of LTP depends not just on glutamate release and postsynaptic depolarization but also on the action of dopamine.

    “studies of LTP (mostly in CA1) show that there is an additional factor: the persistence of LTP depends not just on the two factors of the Hebbian condition (glutamate release and postsynaptic depolarization), but also on a third, the action of the neurotransmitter dopamine [7]”

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Authors
Frey, U., Schroeder, H., & Matthies, H.
Affiliations
Leibniz Institute for Neurobiology (DE)
Corresponding author
Frey, Uwe
Venue
Brain Research
Publisher
Elsevier BV
Published
1990-07
Volume
522
Issue
1
Pages
69-75
ISSN
0006-8993 (ISSN-L)
OpenAlex
W2021485981
Cited by
333
References
43
Citations by year
2026: 2 · 2025: 5 · 2024: 13 · 2023: 3 · 2022: 11 · 2021: 7 · 2020: 5 · 2019: 6 · 2018: 24 · 2017: 10 · 2016: 10 · 2015: 9 · 2014: 18 · 2013: 12 · 2012: 18
FWCI
1.70
Type
journal article
Language
en
Open access
closed
Locations
  • Brain Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
Keywords
long-term potentiation, post-tetanic potentiation, dopamine antagonists, hippocampal CA1, domperidone, apomorphine, sulpiride, flupenthixol, dopamine release, synaptic transmission, LTP maintenance, Schaffer collaterals, postsynaptic mechanisms
MeSH terms
Animals, Apomorphine, Domperidone, Dopamine, Electric Stimulation, Flupenthixol, Hippocampus, Nerve Tissue Proteins, Synaptic Transmission, Sulpiride, Synapses, Dopamine Agents, Dopamine Antagonists, Rats, In Vitro Techniques

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