Cited by 303Listeria monocytogenes in Food SafetyBiochemical and Structural CharacterizationBacterial Genetics and Biotechnology

Structure of Internalin, a Major Invasion Protein of Listeria monocytogenes, in Complex with Its Human Receptor E-Cadherin

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Schubert, Wolf-Dieter, Urbanke, Claus, Ziehm, Thilo, Beier, Viola, Machner, Matthias P., Domann, Eugen, Wehland, Jürgen, Chakraborty, Trinad +1 more·2002·Cell

Abstract

Listeria monocytogenes, a food-borne bacterial pathogen, enters mammalian cells by inducing its own phagocytosis. The listerial protein internalin (InlA) mediates bacterial adhesion and invasion of epithelial cells in the human intestine through specific interaction with its host cell receptor E-cadherin. We present the crystal structures of the functional domain of InlA alone and in a complex with the extracellular, N-terminal domain of human E-cadherin (hEC1). The leucine rich repeat (LRR) domain of InlA surrounds and specifically recognizes hEC1. Individual interactions were probed by mutagenesis and analytical ultracentrifugation. These include Pro16 of hEC1, a major determinant for human susceptibility to L. monocytogenes infection that is essential for intermolecular recognition. Our studies reveal the structural basis for host tro-pism of this bacterium and the molecular deception L. monocytogenes employs to exploit the E-cadherin system.

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  1. [8]8. CCP4 The CCP4 (Collaborative Computational Project 4) suiteprograms for protein crystallography Acta Crystallogr. D. 1994; 50:760-763 Crossref Scopus (19983) PubMed Google Scholar
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  3. [13]13. Evdokimov, A.G. ∙ Anderson, D.E. ∙ Routzahn, K.M. ... Unusual molecular architecture of the Yersinia pesitis cytotoxin YopMa leucine-rich repeat protein with the shortest repeating unit J. Mol. Biol. 2001; 312:807-821 Crossref Scopus (142) PubMed Google Scholar

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Authors
Schubert, W.-D., Urbanke, C., Ziehm, T., Beier, V., Machner, M. P., Domann, E., Wehland, J., Chakraborty, T., & Heinz, D. W.
Affiliations
  • Helmholtz Centre for Infection Research (DE)
  • Medizinische Hochschule Hannover (DE)
  • Justus-Liebig-Universität Gießen (DE)
Corresponding author
Heinz, Dirk W.
Venue
Cell
Publisher
Elsevier BV
Published
2002-12
Volume
111
Issue
6
Pages
825-836
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2019496511
Cited by
302
References
55
Citations by year
2026: 14 · 2025: 8 · 2024: 4 · 2023: 11 · 2022: 9 · 2021: 7 · 2020: 7 · 2019: 9 · 2018: 5 · 2017: 4 · 2016: 6 · 2015: 6 · 2014: 15 · 2013: 13 · 2012: 13
FWCI
16.42
Type
journal article
Language
en
Open access
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Listeria monocytogenes in Food Safety
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Listeria monocytogenes, internalin A, integrated nested Laplace approximation, E-cadherin, bacterial invasion, host cell receptors, leucine-rich repeat domain, protein-protein interaction, host tropism, host susceptibility to infection, epithelial cell adhesion, crystal structure
MeSH terms
Amino Acid Sequence, Bacterial Proteins, Cloning, Molecular, Cytoplasm, Humans, Intestinal Mucosa, Ions, Leucine, Listeria monocytogenes, Models, Molecular, Molecular Sequence Data, Mutation, Protein Binding, Ultracentrifugation, X-Ray Diffraction, Cadherins, Sequence Homology, Amino Acid, Protein Structure, Tertiary, Crystallography, X-Ray

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