E-Cadherin Is the Receptor for Internalin, a Surface Protein Required for Entry of L. monocytogenes into Epithelial Cells
Open accessAbstract
We report the first identification of a cellular receptor mediating entry of a gram-positive bacterium into nonphagocytotic cells. By an affinity chromatography approach, we identified E-cadherin as the ligand for internalin, an L. monocytogenes protein essential for entry into epithelial cells. Expression of the chicken homolog of E-cadherin (L-CAM) in transfected fibroblasts dramatically increases entry of L. monocytogenes and promotes that of a recombinant L. innocua strain expressing internalin but does not promote entry of the wild-type noninvasive L. innocua or that of an internalin-deficient mutant of L. monocytogenes. Furthermore, L-CAM-specific antibodies block internalin-mediated entry. In contrast to Salmonella, Listeria enters cells by a mechanism of induced phagocytosis occurring without membrane ruffling. This work reveals a novel type of heterophilic interactions for E-cadherin.
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E-cadherin mediates the endocytosis of Listeria monocytogenes by epithelial cells.
“E-cadherin is known to mediate the endocytosis of Listeria monocytogenes by epithelial cells [41], but it was not previously known to mediate the endocytosis of any fungi.”
From Als3 Is a Candida albicans Invasin That Binds to Cadherins and Induces Endocytosis by Host Cells (2007) · cites this paper as [41]
Internalin A of L. monocytogenes mediates epithelial cell invasion by binding to E-cadherin.
“Interestingly, this family of proteins includes internalin A of L. monocytogenes, which mediates epithelial cell invasion by binding to E-cadherin [41,44].”
From Als3 Is a Candida albicans Invasin That Binds to Cadherins and Induces Endocytosis by Host Cells (2007) · cites this paper as [41]
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- Authors
- Mengaud, J., Ohayon, H., Gounon, P., Mège, R.-M., & Cossart, P.
- Affiliations
- Institut Pasteur (FR)
- Inserm (FR)
- Corresponding author
- Cossart, Pascale
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1996-03
- Volume
- 84
- Issue
- 6
- Pages
- 923-932
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2001553481
- Cited by
- 870
- References
- 1
- Citations by year
- 2026: 13 · 2025: 13 · 2024: 9 · 2023: 18 · 2022: 19 · 2021: 23 · 2020: 24 · 2019: 17 · 2018: 26 · 2017: 14 · 2016: 23 · 2015: 26 · 2014: 41 · 2013: 24 · 2012: 28
- FWCI
- 50.38
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Listeria monocytogenes in Food Safety
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- E-cadherin, internalins, Listeria monocytogenes, Listeria innocua, heterophilic interactions, Gram-positive bacteria
- MeSH terms
- Animals, Antibody Specificity, Bacterial Proteins, Cell Adhesion, Epithelial Cells, Epithelium, Humans, Ligands, Listeria monocytogenes, Mammals, Molecular Sequence Data, Receptors, Cell Surface, Salmonella, Cadherins, Sequence Homology, Amino Acid, Caco-2 Cells
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