The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Role of lectins in the innate immunity of horseshoe crab
Abstract
We have purified five types of lectins, named tachylectins, from circulating hemocytes and hemolymph plasma of the Japanese horseshoe crab, Tachypleus tridentatus. Tachylectin-1 interacts with Gram-negative bacteria probably through 2-keto-3-deoxyoctonate, one of the constituents of lipopolysaccharides (LPS). Tachylectin-1 also binds to polysaccharides such as agarose and dextran with broad specificity. Tachylectin-2 binds to D-GlcNAc or D-GalNAc and recognizes staphylococcal lipoteichoic acids and LPS from several Gram-negative bacteria. In contrast, tachylectins-3 and -4 specifically bind to S-type LPS from several Gram-negative bacteria through a certain sugar moiety on the O-specific polysaccharides (O-antigens). Tachylectin-5 identified in hemolymph plasma has the strongest bacterial agglutinating activity in the five types of tachylectins, and exhibits broad specificity against acetyl group-containing substances. Thus, the innate immune system of horseshoe crab may recognize invading pathogens through a combinatorial method using lectins with different specificities against carbohydrates exposed on pathogens. An encounter of these lectins derived from hemocytes and hemolymph plasma at injured sites, in response to the stimulation of LPS, suggests that they serve synergistically to accomplish an effective host defense against invading microbes and foreign substances.
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“For example, the hemolymph of horseshoe crabs recognizes invaders through a combinatorial approach, using lectins with different specificities against glycans exposed on pathogens (782), allowing these organisms to survive almost unchanged for >100 million years, without the benefit of adaptive immunity.”
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Metadata
- Authors
- Kawabata, S.-I., & Iwanaga, S.
- Affiliations
- Kyushu University (JP)
- Corresponding author
- Kawabata, Shun-ichiro
- Venue
- Developmental & Comparative Immunology
- Publisher
- Elsevier BV
- Published
- 1999-06
- Volume
- 23
- Issue
- 4-5
- Pages
- 391-400
- ISSN
- 0145-305X (ISSN-L)
- OpenAlex
- W2025265837
- Cited by
- 114
- References
- 27
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 3 · 2023: 3 · 2022: 1 · 2021: 4 · 2020: 4 · 2019: 1 · 2018: 6 · 2017: 1 · 2016: 7 · 2015: 7 · 2014: 3 · 2013: 3 · 2012: 4
- FWCI
- 1.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Developmental & Comparative Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Core Research for Evolutional Science and Technology
- Naito Foundation
- Japan Science and Technology Corporation
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Keywords
- lectins, horseshoe crab, Tachypleus tridentatus, innate immunity, hemocytes, lipopolysaccharide recognition, Gram-negative bacteria, bacterial agglutination, O-antigen, lipoteichoic acid, carbohydrate recognition, host defense
- MeSH terms
- Animals, Gram-Negative Bacteria, Hemocytes, Horseshoe Crabs, Immunity, Innate, Lipopolysaccharides, Teichoic Acids, Sequence Alignment, O Antigens, Lectins
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- Kawabata, S.-I., & Iwanaga, S. (1999). Role of lectins in the innate immunity of horseshoe crab. Developmental & Comparative Immunology. https://doi.org/10.1016/s0145-305x(99)00019-1
