The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Molecular properties of apelin: tissue distribution and receptor binding
Open accessAbstract
We analyzed the tissue distribution of apelin mRNA in rats by a quantitative reverse transcription-polymerase chain reaction and that of immunoreactive apelin (ir-apelin) by an enzyme immunoassay (EIA) using a monoclonal antibody. The expression levels of apelin mRNA and ir-apelin seemed to be consistent among tissues: they were highly expressed in the lung and mammary gland. By the combination of gel filtration and EIA, we found that the molecular forms of apelin differ among respective tissues: apelin molecules with sizes close to apelin-36 (long forms) were major components in the lung, testis, and uterus, but both long and short (whose sizes were close to [<Glu(65)]apelin-13) forms were detected in the mammary gland. In Scatchard analyses, the radioiodinated apelin-36 analogue bound to the receptor, APJ, with high affinity. In competitive binding assays, apelin-36 and apelin-19 far more efficiently inhibited the binding of the labeled apelin-36 analogue with APJ than [<Glu(65)]apelin-13. In analyses for the dissociation of apelin from APJ, unlabeled apelin-36 replaced more rapidly the labeled apelin-36 analogue bound with APJ than [<Glu(65)]apelin-13. Our results demonstrate that the long and short forms of apelin differently interact with APJ.
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Cites
Chemokine receptors as HIV-1 coreceptors: roles in viral entry, tropism, and disease
Berger, E. A., Murphy, P. M., & Farber, J. M. (1999) · Annual Review of Immunology · doi:10.1146/annurev.immunol.17.1.657 · Cited by 2,180
Cited by
Apelin, a Newly Identified Adipokine Up-Regulated by Insulin and Obesity
Boucher, J., Masri, B., Daviaud, D., Gesta, S., Guigné, C., Mazzucotelli, A., Castan-Laurell, I., Tack, I., Knibiehler, B., Carpéné, C., et al. (2005) · Endocrinology · doi:10.1210/en.2004-1427 · Cited by 910
Taheri, S., Murphy, K., Cohen, M., Sujkovic, E., Kennedy, A., Dhillo, W., Dakin, C., Sajedi, A., Ghatei, M., & Bloom, S. (2002) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2002.6575 · Cited by 314
Kidoya, H., Ueno, M., Yamada, Y., Mochizuki, N., Nakata, M., Yano, T., Fujii, R., & Takakura, N. (2008) · The EMBO Journal · doi:10.1038/sj.emboj.7601982 · Cited by 265
Self-citationRecinella, L., Orlando, G., Ferrante, C., Chiavaroli, A., Brunetti, L., & Leone, S. (2020) · Frontiers in Physiology · doi:10.3389/fphys.2020.578966 · Cited by 261
Apelin, orexin-A and leptin plasma levels in morbid obesity and effect of gastric banding
Heinonen, M. V., Purhonen, A. K., Miettinen, P., Pääkkönen, M., Pirinen, E., Alhava, E., Åkerman, K., & Herzig, K. H. (2005) · Regulatory Peptides · doi:10.1016/j.regpep.2005.05.003 · Cited by 232
Metadata
- Authors
- Kawamata, Y., Habata, Y., Fukusumi, S., Hosoya, M., Fujii, R., Hinuma, S., Nishizawa, N., Kitada, C., Onda, H., Nishimura, O., & Fujino, M.
- Venue
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
- Publisher
- Elsevier BV
- Published
- 2001-04
- Volume
- 1538
- Issue
- 2-3
- Pages
- 162-171
- ISSN
- 0167-4889 (ISSN-L)
- OpenAlex
- W2129224037
- Cited by
- 521
- References
- 1
- FWCI
- 5.79
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Kawamata, Y., Habata, Y., Fukusumi, S., Hosoya, M., Fujii, R., Hinuma, S., Nishizawa, N., Kitada, C., Onda, H., Nishimura, O., & Fujino, M. (2001). Molecular properties of apelin: tissue distribution and receptor binding. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. https://doi.org/10.1016/s0167-4889(00)00143-9
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