The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Optimizing scale-up fermentation processes
Abstract
There are many aims associated with the optimization of fermentation processes. Optimization is expected to increase the yield of the final product but the process must be compliant with good manufacturing practices, the available equipment and the expected final scale of operation. Dealing with genetically modified microorganisms that overproduce recombinant protein has the advantage that the vast majority of the processes use only three different species, namely Escherichia coli, Saccharomyces cerevisiae and Pichia pastoris. Standard processes for each organism are described in textbooks and serve as a basis for the development of a tailored process. This article outlines the general philosophy that we have devised to ensure an efficient approach of scaling up fermentation processes for biopharmaceutical purposes, in a multidisciplinary environment.
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“Given that the fed-batch fermentation system entails a scale-up process, researchers would expect a higher yield of end-products (Modak et al., 1986; Thiry and Cingolani, 2002; Hewitt and Nienow, 2007).”
From Fermentation Conditions that Affect Clavulanic Acid Production in Streptomyces clavuligerus: A Systematic Review (2016) · cites this paper as Thiry and Cingolani, 2002
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Cites
High cell-density culture of Escherichia coli
Lee, S. Y. (1996) · Trends in biotechnology · doi:10.1016/0167-7799(96)80930-9 · Cited by 893
Physiological responses to mixing in large scale bioreactors
Enfors, S., Jahic, M., Rozkov, A., Xu, B., Hecker, M., Jürgen, B., Krüger, E., Schweder, T., Hamer, G., O'Beirne, D., et al. (2001) · Journal of Biotechnology · doi:10.1016/s0168-1656(00)00365-5 · Cited by 480
Sreekrishna, K. T., Brankamp, R. G., Kropp, K. E., Blankenship, D. T., Tsay, J. T., Smith, P. L., Wierschke, J. D., Subramaniam, A., & Birkenberger, L. A. (1997) · Gene · doi:10.1016/s0378-1119(96)00672-5 · Cited by 432
Posno, M., Leer, R. J., van Luijk, N., van Giezen, M. J., Heuvelmans, P., Lokman, B. C., & Pouwels, P. H. (1991) · Applied and Environmental Microbiology · doi:10.1128/aem.57.6.1822-1828.1991 · Cited by 187
Futcher, A. B., & Cox, B. S. (1984) · Journal of Bacteriology · doi:10.1128/jb.157.1.283-290.1984 · Cited by 182
Regulation of Carbon Metabolism in Saccharomyces cerevisiae and Related Yeasts
Käppeli, O. (1987) · Advances in microbial physiology/Advances in Microbial Physiology · doi:10.1016/s0065-2911(08)60239-8 · Cited by 175
Scheirlinck, T., Mahillon, J., Joos, H., D’Haese, P. C., & Michiels, F. (1989) · Applied and Environmental Microbiology · doi:10.1128/aem.55.9.2130-2137.1989 · Cited by 140
Large scale production of cyclohexanone monooxygenase from Escherichia coli TOP10 pQR239
O’Sullivan, L., Patel, S., Ward, J. M., Woodley, J. M., & Doig, S. D. (2001) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(00)00320-3 · Cited by 125
The parB (hok/sok) Locus of Plasmid R1: A General Purpose Plasmid Stabilization System
Gerdes, K. (1988) · Nature Biotechnology · doi:10.1038/nbt1288-1402 · Cited by 121
Strategies for improving plasmid stability in genetically modified bacteria in bioreactors
Kumar, P. (1991) · Trends in biotechnology · doi:10.1016/0167-7799(91)90090-5 · Cited by 100
Research works printed but not linked: 2
- Murphy, 1977, Design and analysis of industrials experiments, Chem. Eng., 6, 168
- Kwanmin, 1989, Scale-down techniques for fermentation, Biopharm., 2, 30
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Daly, R., & Hearn, M. T. W. (2004) · Journal of Molecular Recognition · doi:10.1002/jmr.687 · Cited by 742
Industrial production of amino acids by coryneform bacteria
Hermann, T. (2003) · Journal of Biotechnology · doi:10.1016/s0168-1656(03)00149-4 · Cited by 606
Guidelines and Cost Analysis for Catalyst Production in Biocatalytic Processes
Tufvesson, P., Ramos, J. L., Nordblad, M., & Woodley, J. M. (2010) · Organic Process Research & Development · doi:10.1021/op1002165 · Cited by 500
Optimization and scale up of industrial fermentation processes
Schmidt, F. R. (2005) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-005-0003-0 · Cited by 362
Endophytes as in vitro production platforms of high value plant secondary metabolites
Venugopalan, A., & Srivastava, S. (2015) · Biotechnology Advances · doi:10.1016/j.biotechadv.2015.07.004 · Cited by 221
Metadata
- Authors
- Thiry, M., & Cingolani, D.
- Affiliations
- Eurogentec (Belgium) (BE)
- Venue
- Trends in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2002-03
- Volume
- 20
- Issue
- 3
- Pages
- 103-105
- ISSN
- 0167-7799 (ISSN-L)
- OpenAlex
- W2001751900
- Cited by
- 102
- References
- 18
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 6 · 2023: 9 · 2022: 6 · 2021: 5 · 2020: 3 · 2019: 2 · 2018: 5 · 2017: 5 · 2016: 2 · 2015: 6 · 2014: 5 · 2013: 6 · 2012: 10
- FWCI
- 0.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Trends in biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Keywords
- fermentation scale-up, process optimization, recombinant protein expression, genetically modified microorganisms, biopharmaceuticals, Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, good manufacturing practices, yield improvement
- MeSH terms
- Culture Media, Escherichia coli, Fermentation, Glucose, Hydrogen-Ion Concentration, Oxygen, Peptones, Pichia, Recombinant Proteins, Saccharomyces cerevisiae, Biomass, Bioreactors, Feedback, Physiological
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