The DOI matches its Crossref record; 34 of the 35 references it cites were found in a registry or an index.
Microglia-specific localisation of a novel calcium binding protein, Iba1
Abstract
Recently it has been shown that mRNA of Iba1 (ionized calcium binding adaptor molecule 1), which was a novel calcium binding protein cDNA-cloned by our group, is specifically expressed in microglia in cultures of rat brain cells [Imai et al. Biophys. Biochem. Res. Commun., 224 (1996) 855-862]. In the present study, immunocytochemical and immunohistochemical examinations demonstrated that Iba1 protein is expressed in microglia alone both in cultured brain cells and in the brain, respectively. In a mixed cell culture of embryonic rat brain, immunocytochemically positive for Iba1 protein were the microglia but it was not detectable in neurons, astroglia, or oligodendroglia. Immunohistochemical staining of adult rat brain sections showed Iba1 protein to be specifically localised in ramified microglia. In addition, immunohistochemical staining and immunoblot analysis of activated microglia in the facial nucleus after facial nerve axotomy shows that expression of Iba1 protein was upregulated and peaked at 7 days. These results indicated that localisation of Iba1 protein is restricted to microglia both in vitro and in vivo, and that Iba1 protein plays a role in regulating the function of microglia, especially in the activated microglia.
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In the citing paper
“Since its first description in 1998 [3], IBA1 has been the most widely used marker for the immunohistochemical analysis of microglia.”
From Beyond Activation: Characterizing Microglial Functional Phenotypes (2021) · cites this paper as [3]
In the citing paper
“In contrast, the studies using cell counting for Iba1, which is expressed by all microglia,58, 77 assess the absolute number of microglia in the samples.”
From Markers of microglia in post-mortem brain samples from patients with Alzheimer’s disease: a systematic review (2018) · cites this paper as [58]
In the citing paper
“Iba1 is a pan-microglial marker whose expression increases with microglial activation,58, 77”
From Markers of microglia in post-mortem brain samples from patients with Alzheimer’s disease: a systematic review (2018) · cites this paper as [58]
In the citing paper
“Iba1 is a microglia specific marker, which expression increases with microglia activation and macrophage transformation [30]”
From APOE Genotype Differentially Modulates Effects of Anti-Aβ, Passive Immunization in APP Transgenic Mice (2017) · cites this paper as [30]
In the citing paper
“In AD mice, sustained microglial depletion with CSF1R inhibitor reduced plaque development.”
From Microglia in neurodegenerative diseases: mechanism and potential therapeutic targets (2023) · cites this paper as [32]
In the citing paper
“These markers have been extensively used to label or define microglia in various systems 24.”
From Efficient derivation of microglia-like cells from human pluripotent stem cells (2016) · cites this paper as [24]
Citations
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Cited by
Sorted by citations
Kettenmann, H., Hanisch, U.-K., Noda, M., & Verkhratsky, A. (2011) · Physiological Reviews · doi:10.1152/physrev.00011.2010 · Cited by 3,547
Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals
Ohsawa, I., Ishikawa, M., Takahashi, K., Watanabe, M., Nishimaki, K., Yamagata, K., Katsura, K., Katayama, Y., Asoh, S., & Ohta, S. (2007) · Nature Medicine · doi:10.1038/nm1577 · Cited by 2,572
Microglia states and nomenclature: A field at its crossroads
Paolicelli, R. C., Sierra, A., Stevens, B., Tremblay, M.-E., Aguzzi, A., Ajami, B., Amit, I., Audinat, E., Bechmann, I., Bennett, M., et al. (2022) · Neuron · doi:10.1016/j.neuron.2022.10.020 · Cited by 1,973
Obesity is associated with hypothalamic injury in rodents and humans.
Thaler, J. P., Yi, C.-X., Schur, E. A., Guyenet, S. J., Hwang, B. H., Dietrich, M. O., Zhao, X., Sarruf, D. A., Izgur, V., Maravilla, K. R., et al. (2012) · Journal of Clinical Investigation · doi:10.1172/jci59660 · Cited by 1,857
Local self-renewal can sustain CNS microglia maintenance and function throughout adult life
Ajami, B., Bennett, J. L., Krieger, C., Tetzlaff, W., & Rossi, F. M. V. (2007) · Nature Neuroscience · doi:10.1038/nn2014 · Cited by 1,643
Metadata
- Authors
- Ito, D., Imai, Y., Ohsawa, K., Nakajima, K., Fukuuchi, Y., & Kohsaka, S.
- Affiliations
- Keio University (JP)
- National Institute of Neuroscience (JP)
- Corresponding author
- Kohsaka, Shinichi
- Venue
- Molecular Brain Research
- Publisher
- Elsevier BV
- Published
- 1998-06
- Volume
- 57
- Issue
- 1
- Pages
- 1-9
- ISSN
- 0169-328X (ISSN-L)
- OpenAlex
- W2053176712
- Cited by
- 1,476
- References
- 35
- Citations by year
- 2026: 56 · 2025: 82 · 2024: 77 · 2023: 88 · 2022: 92 · 2021: 95 · 2020: 85 · 2019: 103 · 2018: 82 · 2017: 78 · 2016: 70 · 2015: 52 · 2014: 78 · 2013: 55 · 2012: 57
- FWCI
- 5.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Keywords
- Iba1, calcium-binding proteins, microglia, microglial activation, facial nerve axotomy, facial nucleus, ramified microglia, rat brain
- MeSH terms
- Animals, Brain, Calcium-Binding Proteins, Cells, Cultured, Facial Nerve, Fluorescent Antibody Technique, Microfilament Proteins, Motor Neurons, Rats, Wistar, Microglia, Axotomy, Rats
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