CoChat
Sign Up
Cited by 149Neuroblastoma Research and TreatmentsRNA Research and SplicingDevelopmental Biology and Gene Regulation

The DOI matches its Crossref record; 30 of the 31 references it cites were found in a registry or an index.

Neuron-specific splicing of zinc finger transcription factor REST/NRSF/XBR is frequent in neuroblastomas and conserved in human, mouse and rat

Palm, Kaia, Metsis, Madis, Timmusk, Tõnis·1999·Molecular Brain Research
DOI 10.1016/s0169-328x(99)00196-5
Open this paper in CoChat — free account

Abstract

Neuron-restrictive silencer factor (NRSF), also known as repressor element RE1 binding transcription factor (REST) or repressor binding to the X2 box (XBR) (REST/NRSF/XBR), is a zinc finger transcription factor that during early embryogenesis is required to repress a subset of neuron-specific genes in non-neural tissues and undifferentiated neural precursors. We have previously shown that splicing within the coding region of rat REST/NRSF/XBR (rREST) generates several different transcripts all of which are expressed in the adult nervous system. rREST transcripts with short neuron-specific exons (exon N) have in-frame stop codons and encode truncated proteins which have an N-terminal repressor domain and weakened DNA binding activity. The aim of this study was to analyze the regulatory mechanisms underlying REST/NRSF/XBR activity in human and mouse as compared to rat. We show that the structure of REST/NRSF/XBR gene and its regulation by neuron-specific splicing is conserved in human, mouse and rat. Expression levels of REST/NRSF/XBR transcripts with the insertion of exon N are increased during the neuronal differentiation of mouse teratocarcinoma PCC7 and rat pheocromocytoma PC12 cells and are high in several human and mouse neuroblastoma cells as compared to the relatively low levels in the developing and adult nervous system. The exclusive expression of the neuronal forms of REST/NRSF/XBR mRNAs in mouse neuroblastoma Neuro-2A cells is not caused by rearrangement of the REST/NRSF/XBR gene nor by mutations in the sequence of the splice sites flanking exon N. These data suggest that changes in REST/NRSF/XBR splicing pattern may result from altered levels of splicing factors reflecting the formation and/or progression of neuroblastoma tumors.

Overview

Integrity signals

Registry

Crossref · 10.1016/s0169-328x(99)00196-5

Cites

30 of 30 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 1

  1. J. Sambrook, E.J. Fritsch, T. Maniatis, Molecular Cloning, Cold Spring Harbor Laboratory Press, 1989.

Cited by

Sorted by citations

See all 149

Related

See all

Metadata

Authors
Palm, K., Metsis, M., & Timmusk, T.
Affiliations
  • Uppsala University (SE)
  • Tallinn University of Technology (EE)
Corresponding author
Timmusk, Tõnis
ORCID
Venue
Molecular Brain Research
Publisher
Elsevier BV
Published
1999-09
Volume
72
Issue
1
Pages
30-39
ISSN
0169-328X (ISSN-L)
DOI
10.1016/s0169-328x(99)00196-5
OpenAlex
W2031374484
Cited by
144
References
31
Citations by year
2025: 3 · 2024: 13 · 2023: 4 · 2022: 4 · 2021: 3 · 2020: 4 · 2019: 1 · 2018: 3 · 2017: 7 · 2016: 5 · 2015: 5 · 2014: 11 · 2013: 9 · 2012: 11
FWCI
3.01
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Molecular Brain Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Oncology › Neuroblastoma Research and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
neuroblastoma, zinc finger transcription factor, alternative splicing, neuronal differentiation, splicing factors, N2a cells, PC12 cells, truncated protein isoform
MeSH terms
RE1-Silencing Transcription Factor, Animals, Base Sequence, Chromosomes, Human, Pair 4, Exons, Glioma, Humans, Molecular Sequence Data, Neoplasm Proteins, Neuroblastoma, Neurons, Repressor Proteins, RNA Splicing, Sequence Homology, Nucleic Acid, Species Specificity, Transcription Factors, Tumor Cells, Cultured, Gene Expression Regulation, Neoplastic, Zinc Fingers, Consensus Sequence, Sequence Alignment, Teratocarcinoma, Protein Isoforms, Mice, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review