Resistance to the anti-proliferative activity of recombinant arginine deiminase in cell culture correlates with the endogenous enzyme, argininosuccinate synthetase
Abstract
Recombinant mycoplasma enzyme, arginine deiminase (rADI), has been proposed as a possible cancer treatment via arginine depletion. However, many cell lines are resistant to rADI-treatment, even though most require arginine for proliferation. We compared eight different cell lines for sensitivity in cell proliferation to the effect of either rADI or arginine deprivation. The activity of argininosuccinate synthetase (AS), the rate-limiting enzyme for converting citrulline to arginine, was also measured. Our results indicate that resistance to rADI-treatment may correlate with cellular AS activity, either constitutive or inducible, allowing cell survival by conversion of the product of the rADI reaction, i.e. citrulline to arginine.
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The use of arginine-catabolizing enzymes may not be an effective therapeutic strategy for all ASS1-deficient cancers, as some ADI-treated tumors have been found to re-express ASS1.
“However, the use of arginine-catabolizing enzymes may not be an effective therapeutic strategy for all ASS1-deficient cancers, as some ADI-treated tumors have been found to re-express ASS1 (Feun et al., 2012; Long et al., 2013; Shen et al., 2003).”
From Targeting Metabolism for Cancer Therapy (2017) · cites this paper as Shen et al., 2003
Cites
Arginine metabolism: nitric oxide and beyond
WU, G., & MORRIS, S. M. (1998) · Biochemical Journal · doi:10.1042/bj3360001 · Cited by 2,811 · see citing works
Use of ?-asparaginase in childhood ALL
Müller, H.-J. (1998) · Critical Reviews in Oncology/Hematology · doi:10.1016/s1040-8428(98)00015-8 · Cited by 458 · see citing works
Sustained Nitric Oxide Production in Macrophages Requires the Arginine Transporter CAT2
Nicholson, B., Manner, C. K., Kleeman, J. E., & MacLeod, C. L. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m010030200 · Cited by 233 · see citing works
Nussler, A. K., Billiar, T. R., Liu, Z. Z., & Morris, S. M. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)42251-4 · Cited by 217 · see citing works
Scott, L., Lamb, J., Smith, S. H., & Wheatley, D. N. (2000) · British Journal of Cancer · doi:10.1054/bjoc.2000.1353 · Cited by 178 · see citing works
Hattori, Y., Campbell, E. B., & Gross, S. S. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36893-x · Cited by 147 · see citing works
Gong, H., Zölzer, F., Recklinghausen, G. V., Havers, W., & Schweigerer, L. (2000) · Leukemia · doi:10.1038/sj.leu.2401763 · Cited by 138 · see citing works
In vivo anti‐tumor activity of arginine deiminase purified from Mycoplasma arginini
Takaku, H., Takase, M., Abe, S., Hayashi, H., & Miyazaki, K. (1992) · International Journal of Cancer · doi:10.1002/ijc.2910510213 · Cited by 123 · see citing works
Miyazaki, K., Takaku, H., Umeda, M., Fujita, T., Huang, W. D., Kimura, T., Yamashita, J., & Horio, T. (1990) · PubMed · Cited by 115 · see citing works
Flam, B. R., Hartmann, P. J., Harrell-Booth, M., Solomonson, L. P., & Eichler, D. C. (2001) · Nitric Oxide · doi:10.1006/niox.2001.0340 · Cited by 103 · see citing works
Takaku, H., Matsumoto, M., Misawa, S., & Miyazaki, K. (1995) · Japanese Journal of Cancer Research · doi:10.1111/j.1349-7006.1995.tb03094.x · Cited by 89 · see citing works
Arginine Deiminase Inhibits Cell Proliferation by Arresting Cell Cycle and Inducing Apoptosis
Gong, H., Zölzer, F., Recklinghausen, G. V., Rössler, J. K., Breit, S., Havers, W., Fotsis, T., & Schweigerer, L. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.1004 · Cited by 76 · see citing works
Recombinant arginine deiminase as a potential anti-angiogenic agent
Beloussow, K., Wang, L., Wu, J., Ann, D. K., & Shen, W. (2002) · Cancer Letters · doi:10.1016/s0304-3835(01)00793-5 · Cited by 71 · see citing works
Self-citationMisawa, S., Aoshima, M., Takaku, H., Matsumoto, M., & Hayashi, H. (1994) · Journal of Biotechnology · doi:10.1016/0168-1656(94)90050-7 · Cited by 69 · see citing works
Wheatley, D. N., Scott, L., Lamb, J., & Smith, S. H. (2000) · Cellular Physiology and Biochemistry · doi:10.1159/000016333 · Cited by 64 · see citing works
[92] Isolation and determination of arginine and citrulline
Oginsky, E. L. (1957) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(57)03434-5 · Cited by 55 · see citing works
Isolation and characterization of argininosuccinate synthetase from human liver
O’Brien, W. E. (1979) · Biochemistry · doi:10.1021/bi00591a015 · Cited by 53 · see citing works
Flagged first, then by citations
1 of the 18 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Targeting Metabolism for Cancer Therapy
Luengo, A., Gui, D. Y., & Vander Heiden, M. G. (2017) · Cell Chemical Biology · doi:10.1016/j.chembiol.2017.08.028 · Cited by 971 · see citing works
Arginine deprivation and argininosuccinate synthetase expression in the treatment of cancer
Delage, B., Fennell, D. A., Nicholson, L., McNeish, I., Lemoine, N. R., Crook, T., & Szlosarek, P. W. (2010) · International Journal of Cancer · doi:10.1002/ijc.25202 · Cited by 441 · see citing works
Cheng, P., Lam, T. L., Lam, W.-M., Tsui, S. M., Cheng, A. W. M., Lo, W. H., & Leung, Y. (2007) · Cancer Research · doi:10.1158/0008-5472.can-06-1945 · Cited by 279 · see citing works
Kang, J. S., DeLuca, P. P., & Lee, K. C. (2009) · Expert Opinion on Emerging Drugs · doi:10.1517/14728210902907847 · Cited by 252 · see citing works
Izzo, F., Marra, P., Beneduce, G., Castello, G., Vallone, P., De Rosa, V., Cremona, F., Ensor, C. M., Holtsberg, F. W., Bomalaski, J. S., et al. (2004) · Journal of Clinical Oncology · doi:10.1200/jco.2004.11.120 · Cited by 250 · see citing works
Arginine Deprivation as a Targeted Therapy for Cancer
Feun, L. G., You, M., Wu, C., Kuo, M. T., Wangpaichitr, M., Spector, S. A., & Savaraj, N. (2008) · Current Pharmaceutical Design · doi:10.2174/138161208784246199 · Cited by 235 · see citing works
Targeting arginine-dependent cancers with arginine-degrading enzymes: opportunities and challenges
Phillips, M. M., Sheaff, M. T., & Szlosarek, P. W. (2013) · Cancer Research and Treatment · doi:10.4143/crt.2013.45.4.251 · Cited by 229 · see citing works
Szlosarek, P. W., Klampatsa, A., Pallaska, A., Sheaff, M. T., Smith, P., Crook, T., Grimshaw, M. J., Steele, J. P. C., Rudd, R., Balkwill, F. R., et al. (2006) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-06-1101 · Cited by 181 · see citing works
Yoon, C., Shim, Y., Kim, E., Lee, J., Won, N., Kim, J., Park, I., Yoon, D., & Min, B. (2007) · International Journal of Cancer · doi:10.1002/ijc.22322 · Cited by 165 · see citing works
Kremer, J. C., Prudner, B. C., Lange, S. E. S., Bean, G. R., Schultze, M., Brashears, C. B., Radyk, M. D., Redlich, N. J., Tzeng, S., Kami, K., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2016.12.077 · Cited by 160 · see citing works
Bowles, T. L., Kim, R., Galante, J., Parsons, C. M., Virudachalam, S., Kung, H., & Bold, R. J. (2008) · International Journal of Cancer · doi:10.1002/ijc.23723 · Cited by 153 · see citing works
Phase I/II study of pegylated arginine deiminase (ADI-PEG 20) in patients with advanced melanoma
Ott, P. A., Carvajal, R. D., Pandit-Taskar, N., Jungbluth, A. A., Hoffman, E. W., Wu, B.-W., Bomalaski, J. S., Venhaus, R., Pan, L., Old, L. J., et al. (2013) · Investigational New Drugs · doi:10.1007/s10637-012-9862-2 · Cited by 146 · see citing works
Kobayashi, E., Masuda, M., Nakayama, R. T., Ichikawa, H., Satow, R., Shitashige, M., Honda, K., Yamaguchi, U., Shoji, A., Tochigi, N., et al. (2010) · Molecular Cancer Therapeutics · doi:10.1158/1535-7163.mct-09-0774 · Cited by 120 · see citing works
Pegylated arginine deiminase: a novel anticancer enzyme agent
Feun, L. G., & Savaraj, N. (2006) · Expert Opinion on Investigational Drugs · doi:10.1517/13543784.15.7.815 · Cited by 118 · see citing works
Kelly, M. P., Jungbluth, A. A., Wu, B.-W., Bomalaski, J. S., Old, L. J., & Ritter, G. (2011) · British Journal of Cancer · doi:10.1038/bjc.2011.524 · Cited by 117 · see citing works
Feun, L. G., Marini, A., Walker, G., Elgart, G., Moffat, F., Rodgers, S. E., Wu, C. J., You, M., Wangpaichitr, M., Kuo, M. T., et al. (2012) · British Journal of Cancer · doi:10.1038/bjc.2012.106 · Cited by 113 · see citing works
Lam, A. T., Wong, G., Chow, H., Chong, H.-C., Chow, T. L., Kwok, S.-Y., Cheng, P. N.-M., Wheatley, D. N., Lo, W., & Leung, Y. (2010) · Pigment Cell & Melanoma Research · doi:10.1111/j.1755-148x.2010.00798.x · Cited by 99 · see citing works
Amino acid deprivation using enzymes as a targeted therapy for cancer and viral infections
Fernandes, H. S., Teixeira, C. S. S., Fernandes, P. A., Ramos, M. J., & Cerqueira, N. M. F. S. A. (2016) · Expert Opinion on Therapeutic Patents · doi:10.1080/13543776.2017.1254194 · Cited by 91 · see citing works
Microbial-based therapy of cancer: Current progress and future prospects
Bernardes, N., Seruca, R., Chakrabarty, A. M., & Fialho, A. M. (2010) · Bioengineered Bugs · doi:10.4161/bbug.1.3.10903 · Cited by 88 · see citing works
Alexandrou, C., Al-Aqbi, S. S., Higgins, J. A., Boyle, W., Karmokar, A., Andreadi, C., Luo, J., Moore, D. A., Viskaduraki, M., Blades, M., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-30591-7 · Cited by 86 · see citing works
Lam, T. L., Wong, G., Chong, H.-C., Cheng, P. N.-M., Choi, S. C., Chow, T. L., Kwok, S.-Y., Poon, R. T. P., Wheatley, D. N., Lo, W., et al. (2009) · Cancer Letters · doi:10.1016/j.canlet.2008.11.031 · Cited by 87 · see citing works
Mimi, H., Ho, K. M., Siu, Y. S., Wu, A., & Li, P. (2011) · Journal of Controlled Release · doi:10.1016/j.jconrel.2011.10.035 · Cited by 79 · see citing works
Wheatley, D. N. (2004) · Anti-Cancer Drugs · doi:10.1097/00001813-200410000-00002 · Cited by 74 · see citing works
Wheatley, D. N. (2005) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2005.04.002 · Cited by 62 · see citing works
Shen, L., Beloussow, K., & Shen, W. (2004) · Biochemical Pharmacology · doi:10.1016/j.bcp.2004.09.003 · Cited by 60 · see citing works
Self-citationShen, L., Beloussow, K., & Shen, W. (2005) · Cancer Letters · doi:10.1016/j.canlet.2005.01.007 · Cited by 59 · see citing works
Self-citationThe arginine metabolism and its deprivation in cancer therapy
Feng, T., Xie, F., Lyu, Y., Yu, P., Chen, B., Yu, J., Zhang, G., To, K. F., Tsang, C. M., & Kang, W. (2025) · Cancer Letters · doi:10.1016/j.canlet.2025.217680 · Cited by 52 · see citing works
Yeh, T.-H., Chen, Y.-R., Chen, S., Shen, W., Ann, D. K., Zaro, J. L., & Shen, L. (2015) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.5b00706 · Cited by 53 · see citing works
Self-citationZeng, X. L., Li, Y., Fan, J., Zhao, H., Xian, Z., Sun, Y., Wang, Z., Wang, S.-G., Zhang, G., & Ju, D. (2013) · Cell Death and Disease · doi:10.1038/cddis.2013.359 · Cited by 51 · see citing works
Rotondo, R., Mastracci, L., Piazza, T., Barisione, G., Fabbi, M., Cassanello, M., Costa, R., Morandi, B., Astigiano, S., Cesario, A., et al. (2008) · International Journal of Cancer · doi:10.1002/ijc.23437 · Cited by 45 · see citing works
Arginine deprivation as a new treatment strategy for head and neck cancer
Huang, C., Tsai, S., Kuo, C., Chang, J. S.-M., Jin, Y., Chang, J., & Hsiao, J. (2012) · Oral Oncology · doi:10.1016/j.oraloncology.2012.06.004 · Cited by 41 · see citing works
Noh, E., Kang, S., Shin, Y., Choi, S., Kim, C., Park, I.-S., Wheatley, D. N., & Min, B. (2004) · International Journal of Cancer · doi:10.1002/ijc.20435 · Cited by 40 · see citing works
Burrows, N., Cane, G., Robson, M. P., Gaude, E., Howat, W., Szlosarek, P. W., Pedley, R. B., Frezza, C., Ashcroft, M. A., & Maxwell, P. H. (2016) · Scientific Reports · doi:10.1038/srep22950 · Cited by 37 · see citing works
Agrawal, V., Woo, J. H., Mauldin, J., Jo, C., Stone, E. M., Georgiou, G., & Frankel, A. E. (2011) · Anti-Cancer Drugs · doi:10.1097/cad.0b013e32834ae42b · Cited by 34 · see citing works
The relationship of arginine deprivation, argininosuccinate synthetase and cell death in melanoma.
Savaraj, N., Wu, C., Kuo, M. T., You, M., Wangpaichitr, M., Robles, C., Spector, S. A., & Feun, L. G. (2007) · Europe PMC (PubMed Central) · Cited by 32 · see citing works
Shen, L., Lin, W., Beloussow, K., Hosoya, K., Terasaki, T., Ann, D. K., & Shen, W. (2003) · Biochemical Pharmacology · doi:10.1016/s0006-2952(03)00555-0 · Cited by 32 · see citing works
Self-citationArginine depriving enzymes: applications as emerging therapeutics in cancer treatment
Kumari, N., & Bansal, S. (2021) · Cancer Chemotherapy and Pharmacology · doi:10.1007/s00280-021-04335-w · Cited by 30 · see citing works
The Relationship of Arginine Deprivation, Argininosuccinate Synthetase and Cell Death in Melanoma
Savaraj, N., Wu, C., Kuo, M. T., You, M., Wangpaichitr, M., Robles, C., Spector, S. A., & Feun, L. G. (2007) · Drug Target Insights · doi:10.1177/117739280700200016 · Cited by 30 · see citing works
Yu, H., Wu, F. L., Lin, S., & Shen, L. (2008) · Journal of Neuroscience Research · doi:10.1002/jnr.21740 · Cited by 29 · see citing works
Self-citationKhalil, N., & Abi‐Habib, R. J. (2019) · Investigational New Drugs · doi:10.1007/s10637-019-00883-4 · Cited by 27 · see citing works
Anti-tumor activity of arginine deiminase via arginine deprivation in retinoblastoma
Kim, J. H., Kim, J. H., Yu, Y. S., Kim, D. W., Min, B.-H., & Kim, K.-W. (2007) · Oncology Reports · doi:10.3892/or.18.6.1373 · Cited by 26 · see citing works
Treating liver cancer through arginine depletion
Prasad, Y. R., Anakha, J., & Pande, A. H. (2024) · Drug Discovery Today · doi:10.1016/j.drudis.2024.103940 · Cited by 27 · see citing works
An Engineered Arginase FC Protein Inhibits Tumor GrowthIn VitroandIn Vivo
Li, L., Wang, Y., Chen, J., Cheng, B., Hu, J., Zhou, Y., Gao, X., Gao, L., Mei, X., Sun, M., et al. (2013) · Evidence-based Complementary and Alternative Medicine · doi:10.1155/2013/423129 · Cited by 24 · see citing works
Lai, H.-T., Naumova, N., Marchais, A., Gaspar, N., Geoerger, B., & Brenner, C. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.948097 · Cited by 23 · see citing works
Arginine Is a Novel Drug Target for Arginine Decarboxylase in Human Colorectal Cancer Cells
Wei, X., Chow, H., Chong, H.-C., Leung, S.-L., Ho, M.-K., Lee, L. M.-Y., & Leung, Y. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241813741 · Cited by 18 · see citing works
Park, H., Lee, J., Shim, Y.-J., Shin, Y., Jeong, S.-Y., Oh, J., Park, G. H., Lee, K.-H., & Min, B.-H. (2008) · Molecules and Cells · doi:10.1016/s1016-8478(23)17586-8 · Cited by 17 · see citing works
Wu, F. L., Yeh, T.-H., Chen, Y.-L., Chiu, Y.-C., Cheng, J.-C., Wei, M., & Shen, L. (2014) · Molecular Pharmaceutics · doi:10.1021/mp5001372 · Cited by 16 · see citing works
Self-citationHuang, Z., & Hu, H. (2021) · Molecules · doi:10.3390/molecules26020511 · Cited by 16 · see citing works
Li, F., Ning, H., Duan, X., Chen, Z., & Xu, L. (2020) · Domestic Animal Endocrinology · doi:10.1016/j.domaniend.2020.106537 · Cited by 14 · see citing works
Walts, A. E., Bomalaski, J. S., Ines, D., & Oršulić, S. (2014) · Journal of Cancer Research and Clinical Oncology · doi:10.1007/s00432-014-1904-z · Cited by 12 · see citing works
Zarei, M., Rahbar, M. R., Negahdaripour, M., Morowvat, M. H., Nezafat, N., & Ghasemi, Y. (2019) · Current Proteomics · doi:10.2174/1570164616666190701120351 · Cited by 11 · see citing works
Wu, F. L., Liang, Y.-F., Chang, Y.-C., Yo, H.-H., Wei, M., & Shen, L. (2011) · Journal of Biomedical Science · doi:10.1186/1423-0127-18-25 · Cited by 9 · see citing works
Self-citationDependence of leukemic cell proliferation and survival on H2O2 and l-arginine
Brown, R. D., Burke, G. A. A., & Brown, G. C. (2009) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2009.02.001 · Cited by 8 · see citing works
Cancer therapies based on enzymatic amino acid depletion
Teixeira, C. S. S., Fernandes, H. S., Fernandes, P. A., Ramos, M. J., & Cerqueira, N. M. F. S. A. (2017) · Elsevier eBooks · doi:10.1016/b978-0-323-46144-3.00024-6 · Cited by 6 · see citing works
Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling
Seim, G. L., Britt, E. C., & Fan, J. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9236-2_13 · Cited by 4 · see citing works
Lee, M.-J., Kim, D. W., Lee, Y.-P., Jeong, H.-J., Kang, H.-W., Shin, M.-J., Sohn, E. J., Kim, M. J., Jang, S.-H., Kang, T., et al. (2009) · BMB Reports · doi:10.5483/bmbrep.2009.42.5.286 · Cited by 3 · see citing works
Increased L -arginine transport via system b0,+ in human proximal tubular cells exposed to albumin
Ashman, N., Brunini, T. M. C., Mann, G. E., Ribeiro, A. C. M., & Yaqoob, M. M. (2006) · Clinical Science · doi:10.1042/cs20060158 · Cited by 3 · see citing works
The Relationship of Arginine Deprivation, Argininosuccinate Synthetase and Cell Death in Melanoma
Savaraj, N., Wu, C., Kuo, M. T., You, M., Wangpaichitr, M., Robles, C., Spector, S. A., & Feun, L. G. (2007) · Drug Target Insights · doi:10.33393/dti.2007.1314 · Cited by 2 · see citing works
Energetics of whole genome doubling and genomic instability
Beck, R. J., Tagal, V., Amin, S. B., Bakhoum, S. F., Maley, C. C., Verhaak, R. G. W., Gomes, A. P., & Andor, N. (2025) · Cancer Letters · doi:10.1016/j.canlet.2025.217878 · Cited by 1 · see citing works
Arginine Deiminase and Cancer Therapy
Feun, L. G., Kuo, M. T., You, M., Wu, C. J., Wangpaichitr, M., & Savaraj, N. (2010) · doi:10.1002/9780470626528.ch9 · Cited by 1 · see citing works
Li, P., Siu, Y. S., Ho, K. M., & Li, W. (2013) · Regenerative medicine, artificial cells and nanomedicine · doi:10.1142/9789814472869_0018
Kobayashi, E., Masuda, M., Nakayama, R. T., Ichikawa, H., Satow, R., Shitashige, M., Honda, K., Yamaguchi, U., Shoji, A., Tochigi, N., et al. (2023) · doi:10.1158/1535-7163.c.6531447
Kobayashi, E., Masuda, M., Nakayama, R. T., Ichikawa, H., Satow, R., Shitashige, M., Honda, K., Yamaguchi, U., Shoji, A., Tochigi, N., et al. (2023) · doi:10.1158/1535-7163.c.6531447.v1
Taki, F., Farhat, M. H., Atat, O. E., Al-Samman, S., Assaf, N., & Abi‐Habib, R. J. (2026) · Human Cell · doi:10.1007/s13577-026-01370-8
Abdelkhalek, M., El-Jawhari, Y., Shaheen, M., Saad, R., Sarieddine, M., El-Husseini, N., El‐Sibai, M., & Abi‐Habib, R. J. (2026) · CNS Oncology · doi:10.1080/20450907.2026.2666026
- Authors
- Shen, L.-J., Lin, W.-C., Beloussow, K., & Shen, W.-C.
- Affiliations
- University of Southern California (US)
- Corresponding author
- Shen, Wei-Chiang
- Venue
- Cancer Letters
- Publisher
- Elsevier BV
- Published
- 2003-03
- Volume
- 191
- Issue
- 2
- Pages
- 165-170
- ISSN
- 0304-3835 (ISSN-L)
- OpenAlex
- W2021227763
- Cited by
- 66
- References
- 18
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 4 · 2012: 3
- FWCI
- 2.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cancer Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Cancer Research and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Polyamine Metabolism and Applications
- Keywords
- arginine deiminase, argininosuccinate synthetase, arginine deprivation, tumor cell proliferation, drug resistance, recombinant enzyme, cell lines
- MeSH terms
- Animals, Arginine, Argininosuccinate Synthase, Cell Division, Cell Line, Cell Survival, Citrulline, Dogs, Drug Resistance, Cricetinae, Humans, Hydrolases, Mycoplasma, Recombinant Proteins, Mice
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