Cited by 66Cancer Research and TreatmentsAmino Acid Enzymes and MetabolismPolyamine Metabolism and Applications

Resistance to the anti-proliferative activity of recombinant arginine deiminase in cell culture correlates with the endogenous enzyme, argininosuccinate synthetase

Shen, Li-Jiuan, Lin, Wen-Chun, Beloussow, Karin, Shen, Wei-Chiang·2003·Cancer Letters

Abstract

Recombinant mycoplasma enzyme, arginine deiminase (rADI), has been proposed as a possible cancer treatment via arginine depletion. However, many cell lines are resistant to rADI-treatment, even though most require arginine for proliferation. We compared eight different cell lines for sensitivity in cell proliferation to the effect of either rADI or arginine deprivation. The activity of argininosuccinate synthetase (AS), the rate-limiting enzyme for converting citrulline to arginine, was also measured. Our results indicate that resistance to rADI-treatment may correlate with cellular AS activity, either constitutive or inducible, allowing cell survival by conversion of the product of the rADI reaction, i.e. citrulline to arginine.

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Crossref · 10.1016/s030-43835(02)00693-6

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  • The use of arginine-catabolizing enzymes may not be an effective therapeutic strategy for all ASS1-deficient cancers, as some ADI-treated tumors have been found to re-express ASS1.

    “However, the use of arginine-catabolizing enzymes may not be an effective therapeutic strategy for all ASS1-deficient cancers, as some ADI-treated tumors have been found to re-express ASS1 (Feun et al., 2012; Long et al., 2013; Shen et al., 2003).”

    From Targeting Metabolism for Cancer Therapy (2017) · cites this paper as Shen et al., 2003

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Authors
Shen, L.-J., Lin, W.-C., Beloussow, K., & Shen, W.-C.
Affiliations
University of Southern California (US)
Corresponding author
Shen, Wei-Chiang
Venue
Cancer Letters
Publisher
Elsevier BV
Published
2003-03
Volume
191
Issue
2
Pages
165-170
ISSN
0304-3835 (ISSN-L)
OpenAlex
W2021227763
Cited by
66
References
18
Citations by year
2026: 2 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 4 · 2012: 3
FWCI
2.94
Type
journal article
Language
en
Open access
closed
Locations
  • Cancer Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Cancer Research and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Polyamine Metabolism and Applications
Keywords
arginine deiminase, argininosuccinate synthetase, arginine deprivation, tumor cell proliferation, drug resistance, recombinant enzyme, cell lines
MeSH terms
Animals, Arginine, Argininosuccinate Synthase, Cell Division, Cell Line, Cell Survival, Citrulline, Dogs, Drug Resistance, Cricetinae, Humans, Hydrolases, Mycoplasma, Recombinant Proteins, Mice

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