The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Proliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells
Open accessAbstract
Bone marrow stromal cells (BMSC) are an attractive target for novel strategies in the gene/cell therapy of hematologic and skeletal pathologies, involving BMSC in vitro expansion/transfection and reinfusion. We investigated the effects of in vitro expansion on BMSC pluripotentiality, proliferative ability, and bone-forming efficiency in vivo. BMSC from three marrow donors were cultured to determine their growth kinetics. At each passage, their differentiation potential was verified by culture in inductive media and staining with alizarin red, alcian blue, or Sudan black, and by immunostaining for osteocalcin or collagen II. First passage cells were compared to fresh marrow for their bone-forming efficiency in vivo. Stromal cell clones were isolated from five donors and characterized for their multidifferentiation ability. The lifespan and differentiation kinetics of five of these clones were determined. After the first passage, BMSC had a markedly diminish proliferation rate and gradually lost their multiple differentiation potential. Their bone-forming efficiency in vivo was reduced by about 36 times at first confluence as compared to fresh bone marrow. Experiments on the clones yielded comparable results. Culture expansion causes BMSC to gradually lose their early progenitor properties. Both the duration and the conditions of culture could be crucial to successful clinical use of these cells and must be considered when designing novel therapeutic strategies involving stromal mesenchymal progenitor manipulation and reinfusion.
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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 46,426
Caplan, A. I. (1991) · Journal of Orthopaedic Research · doi:10.1002/jor.1100090504 · Cited by 6,548
In VitroChondrogenesis of Bone Marrow-Derived Mesenchymal Progenitor Cells
Johnstone, B., Hering, T. M., Caplan, A. I., Goldberg, V. M., & Yoo, J. U. (1998) · Experimental Cell Research · doi:10.1006/excr.1997.3858 · Cited by 2,382
Horwitz, E. M., Prockop, D. J., Fitzpatrick, L. A., Koo, W. W. K., Gordon, P. L., Neel, M., Sussman, M., Orchard, P., Marx, J. C., Pyeritz, R. E., et al. (1999) · Nature Medicine · doi:10.1038/6529 · Cited by 1,824
Bruder, S. P., Jaiswal, N., & Haynesworth, S. E. (1997) · Journal of Cellular Biochemistry · doi:10.1002/(sici)1097-4644(199702)64:2<278::aid-jcb11>3.0.co;2-f · Cited by 1,485
Friedenstein, A. J., Chailakhyan, R. K., Latsinik, N. V., Panasyuk, A. F., & Keiliss-Borok, I. V. (1974) · Transplantation · doi:10.1097/00007890-197404000-00001 · Cited by 1,431
Regulatory Mechanisms in Stem Cell Biology
Morrison, S. J., Shah, N. M., & Anderson, D. J. (1997) · Cell · doi:10.1016/s0092-8674(00)81867-x · Cited by 1,043
Beresford, J. N., Bennett, J. H., Devlin, C., Leboy, P. S., & Owen, M. E. (1992) · Journal of Cell Science · doi:10.1242/jcs.102.2.341 · Cited by 836
Owen, M. (1988) · Journal of Cell Science · doi:10.1242/jcs.1988.supplement_10.5 · Cited by 830
Pereira, R. F., O’Hara, M. D., Laptev, A. V., Halford, K. W., Pollard, M. D., Class, R., Simon, D., Livezey, K., & Prockop, D. J. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.3.1142 · Cited by 544
Cited by
Horwitz, E. M., Gordon, P. L., Koo, W. K. K., Marx, J. C., Neel, M. D., McNall, R. Y., Muul, L., & Hofmann, T. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.132252399 · Cited by 1,650
Stolzing, A., Jones, E., McGonagle, D., & Scutt, A. (2008) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2007.12.002 · Cited by 1,195
Mesenchymal stem cells and immunomodulation: current status and future prospects
Gao, F., Chiu, S. M., Motan, D. A. L., Zhang, Z., Chen, L., Ji, H.-L., Tse, H.-F., Fu, Q.-L., & Lian, Q. (2016) · Cell Death & Disease · doi:10.1038/cddis.2015.327 · Cited by 1,121
Baxter, M. A., Wynn, R. F., Jowitt, S. N., Wraith, J. E., Fairbairn, L. J., & Bellantuono, I. (2004) · STEM CELLS · doi:10.1634/stemcells.22-5-675 · Cited by 771
Immunomodulation by mesenchymal stem cells and clinical experience
Le Blanc, K., & Ringdén, O. (2007) · Journal of Internal Medicine · doi:10.1111/j.1365-2796.2007.01844.x · Cited by 720
Metadata
- Authors
- Banfi, A., Muraglia, A., Dozin, B., Mastrogiacomo, M., Cancedda, R., & Quarto, R.
- Venue
- Experimental Hematology
- Publisher
- Elsevier BV
- Published
- 2000-06
- Volume
- 28
- Issue
- 6
- Pages
- 707-715
- ISSN
- 0301-472X (ISSN-L)
- OpenAlex
- W1566246481
- Cited by
- 730
- References
- 24
- FWCI
- 5.14
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Banfi, A., Muraglia, A., Dozin, B., Mastrogiacomo, M., Cancedda, R., & Quarto, R. (2000). Proliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells. Experimental Hematology. https://doi.org/10.1016/s0301-472x(00)00160-0
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