A sensitive polymerase chain reaction-based assay for the detection of Setaria digitata: The causative organism of cerebrospinal nematodiasis in goats, sheep and horses
Abstract
A sensitive PCR assay for the detection of Setaria digitata has been developed. Two oligonucleotide primers (17 nt) were designed from a previously cloned and characterized tandemly arranged repetitive sequence of Setaria digitata. Using these primers, it was possible to amplify small quantities (100 fg) of S. digitata genomic DNA. A simple procedure, using proteinase K and non-ionic detergent NP 40, was followed to process the host blood samples and mosquitoes harbouring L3 larvae. The sensitivity of the polymerase chain reaction based assay surpasses the microscopic detection and the previously reported oligonucleotide based chemiluminescent detection of microfilariae in infected host blood samples and L3 larvae in mosquitoes.
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Setariosis caused by different Setaria spp. is a filarial disease affecting bovines and can accidentally be transmitted into unusual hosts such as horses, donkeys, sheep, goats, and camels.
“Setariosis caused by different Setaria spp. is a filarial disease affecting bovines and can accidentally be transmitted into unusual hosts such as horses, donkeys, sheep, goats, and camels [38,39,40,41].”
From Emergent and Neglected Equine Filariosis in Egypt: Species Diversity and Host Immune Response (2022) · cites this paper as [38]
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- Authors
- Wijesundera, W. S. S., Chandrasekharan, N. V., & Karunanayake, E. H.
- Affiliations
- University of Colombo (LK)
- Corresponding author
- Karunanayake, Eric H.
- Venue
- Veterinary Parasitology
- Publisher
- Elsevier BV
- Published
- 1999-03
- Volume
- 81
- Issue
- 3
- Pages
- 225-233
- ISSN
- 0304-4017 (ISSN-L)
- OpenAlex
- W2047851267
- Cited by
- 18
- References
- 16
- Citations by year
- 2022: 3 · 2021: 1 · 2020: 3 · 2017: 1 · 2016: 1 · 2012: 1
- FWCI
- 0.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Veterinary Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
- Keywords
- Setaria digitata, cerebrospinal nematodiasis, PCR detection, primer sequences, repetitive DNA, third-stage larvae, mosquitoes, goats, sheep, horses, microfilariae, diagnostic sensitivity
- MeSH terms
- Animals, Cattle, Detergents, Electrophoresis, Agar Gel, Goats, Horse Diseases, Horses, Microfilariae, Polyethylene Glycols, Sensitivity and Specificity, Setariasis, Sheep, Sheep Diseases, Sri Lanka, Goat Diseases, Polymerase Chain Reaction, Setaria Nematode, Octoxynol, DNA Primers, DNA, Helminth, Endopeptidase K
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