Carotenoid:methyl-β-cyclodextrin formulations: an improved method for supplementation of cultured cells
Abstract
BC. Two days after supplementation with 5 microM BC in MbetaCD, cellular BC levels reached a maximum of 140+/-11 pmol/microg DNA, leveling off to 100+/-15 pmol/microg DNA until day 8. Incubation with BC dissolved in THF/DMSO resulted in a lower BC uptake of 105+/-14 pmol/microg DNA and 64+/-20 pmol/microg DNA respectively. No cytotoxic effects of these formulations were detected. The results show that the MbetaCD formulation is an improved method for investigations of carotenoids and other lipophilic compounds in in vitro test systems compared to methods using organic solvents.
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Pfitzner et al. demonstrated that methyl-β-CD was an improved vehicle for the investigation of carotenoids and other lipophilic compounds in in vitro test systems compared to organic solvents.
“Pfitzner et al. [18] have demonstrated that methyl-β-CD (M-β-CD) was an improved vehicle for the investigation of carotenoids and other lipophilic compounds in in vitro test systems, compared to organic solvents.”
From The Effect of Lycopene Preexposure on UV‐B‐Irradiated Human Keratinocytes (2016) · cites this paper as [18]
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The Effect of Lycopene Preexposure on UV‐B‐Irradiated Human Keratinocytes
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- Authors
- Pfitzner, I., Francz, P. I., & Biesalski, H. K.
- Affiliations
- University of Hohenheim (DE)
- Corresponding author
- Biesalski, Hans Konrad
- Venue
- Biochimica et Biophysica Acta (BBA) - General Subjects
- Publisher
- Elsevier BV
- Published
- 2000-04
- Volume
- 1474
- Issue
- 2
- Pages
- 163-168
- ISSN
- 0304-4165 (ISSN-L)
- OpenAlex
- W1982982732
- Cited by
- 58
- References
- 24
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 1 · 2021: 3 · 2020: 1 · 2017: 2 · 2016: 2 · 2015: 6 · 2013: 3 · 2012: 6
- FWCI
- 3.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochimica et Biophysica Acta (BBA) - General Subjects (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Università degli Studi di Camerino
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Drug Solubulity and Delivery Systems
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Keywords
- carotenoids, methyl-β-cyclodextrin, beta-carotene, zeaxanthin, lutein, lycopene, bioavailability, cellular uptake, organic solvent toxicity, human dermal fibroblasts, formulation stability
- MeSH terms
- Carotenoids, Cell Survival, Cells, Cultured, Culture Media, Cyclodextrins, Skin, beta-Cyclodextrins
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