Differential messenger RNA distribution of lactate dehydrogenase LDH-1 and LDH-5 isoforms in the rat brain
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The role of lactate in brain energy metabolism has recently received renewed attention. Although blood-borne monocarboxylates such as lactate poorly cross the blood-brain barrier in the adult brain, lactate produced within the brain parenchyma may be a suitable substrate for brain cells. Lactate dehydrogenase is crucial for both the production and utilization of lactate. In this article, we report the regional distribution of the messenger RNAs for lactate dehydrogenase isoforms 1 and 5 in the adult rat brain using in situ hybridization histochemistry with specific [alpha-(35)S]dATP 3' end-labeled oligoprobes. The autoradiographs revealed that the lactate dehydrogenase-1 messenger RNA is highly expressed in a variety of brain structures, including the main olfactory bulb, the piriform cortex, several thalamic and hypothalamic nuclei, the pontine nuclei, the ventral cochlear nucleus, the trigeminal nerve and the solitary tractus nucleus. In addition, the granular and Purkinje cell layers of the cerebellum showed a strong labeling. The neocortex (e.g., cingular, retrosplenial and frontoparietal cortices) often exhibits a marked laminar pattern of distribution of lactate dehydrogenase-1 messenger RNA (layers II/III, IV and VI being most strongly labeled). In contrast, expression of the lactate dehydrogenase-5 messenger RNA generally seemed more diffusely distributed across the different brain regions. Expression was particularly strong in the hippocampal formation (especially in…
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Ldha gene expression was strongest in the cerebral cortex and hippocampus.
“Ldha gene expression was strongest in the cerebral cortex and hippocampus as previously reported (Laughton et al., 2000;Ross et al., 2010) (Figure 2g).”
From Downregulated expression of lactate dehydrogenase in adult oligodendrocytes and its implication for the transfer of glycolysis products to axons (2024) · cites this paper as Laughton et al., 2000
Ldhb mRNA was prominent in neurons of the basal forebrain, including several thalamic nuclei and in the midbrain.
“The more widely expressed Ldhb mRNA was also prominent in neurons of the basal forebrain, including several thalamic nuclei and in the midbrain as described (Laughton et al., 2000;Ross et al., 2010) (Figure 2g).”
From Downregulated expression of lactate dehydrogenase in adult oligodendrocytes and its implication for the transfer of glycolysis products to axons (2024) · cites this paper as Laughton et al., 2000
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Pellerin, L., Bouzier‐Sore, A., Aubert, A., Serres, S., Merle, M., Costalat, R., & Magistretti, P. J. (2007) · Glia · doi:10.1002/glia.20528 · Cited by 805 · see citing works
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Mächler, P., Wyss, M. T., Elsayed, M., Stobart, J., Gutierrez, R., von Faber-Castell, A., Kaelin, V., Zuend, M., San Martín, A., Romero-Gómez, I., et al. (2016) · Cell Metabolism · doi:10.1016/j.cmet.2015.10.010 · Cited by 571 · see citing works
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The redox switch/redox coupling hypothesis
Cerdán, S., Rodrigues, T. B., Sierra, A., Benito, M., Fonseca, L. L., Fonseca, C. P., & García-Martín, M. L. (2006) · Neurochemistry International · doi:10.1016/j.neuint.2005.12.036 · Cited by 146 · see citing works
Späte, E., Zhou, B., Sun, T., Kusch, K., Asadollahi, E., Siems, S. B., Depp, C., Werner, H. B., Saher, G., Hirrlinger, J., et al. (2024) · Glia · doi:10.1002/glia.24533 · Cited by 40 · see citing works
- Authors
- Laughton, J. D., Charnay, Y., Belloir, B., Pellerin, L., Magistretti, P. J., & Bouras, C.
- Affiliations
- University Hospital of Geneva (CH)
- Centre Hospitalier Universitaire Vaudois (CH)
- Corresponding author
- Laughton, J.D.
- Venue
- Neuroscience
- Publisher
- Elsevier BV
- Published
- 2000-03
- Volume
- 96
- Issue
- 3
- Pages
- 619-625
- ISSN
- 0306-4522 (ISSN-L)
- OpenAlex
- W2053390767
- Cited by
- 77
- References
- 1
- Citations by year
- 2025: 3 · 2024: 6 · 2023: 5 · 2022: 2 · 2021: 5 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 4 · 2016: 1 · 2015: 4 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 1.60
- Type
- journal article
- Language
- en
- Open access
- green
- infoscience.epfl.ch
- Locations
- Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- lactate dehydrogenase, LDH1, LDH5, mRNA distribution, brain energy metabolism, lactate, regional gene expression, cerebellum, hippocampus, cerebral cortex, neocortex, thalamus, hypothalamus, olfactory bulb
- MeSH terms
- Animals, Autoradiography, Brain, Histocytochemistry, Isoenzymes, L-Lactate Dehydrogenase, Male, RNA, Messenger, Tissue Distribution, Rats, Wistar, In Situ Hybridization, Rats
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