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Cited by 1,013

The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.

Endoproteolysis of Presenilin 1 and Accumulation of Processed Derivatives In Vivo

Open access
Thinakaran, Gopal, Borchelt, David R, Lee, Michael K, Slunt, Hilda H, Spitzer, Lia, Kim, Grace, Ratovitsky, Tamara, Davenport, Frances, Nordstedt, Christer, Seeger, Mary, Hardy, John, Levey, Allan I, Gandy, Samuel E, Jenkins, Nancy A, Copeland, Neal G, Price, Donald L, Sisodia, Sangram S·1996·Neuron
DOI 10.1016/s0896-6273(00)80291-3
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Abstract

The majority of early-onset cases of familial Alzheimer's disease (FAD) are linked to mutations in two related genes, PS1 and PS2, located on chromosome 14 and 1, respectively. Using two highly specific antibodies against nonoverlapping epitopes of the PS1-encoded polypeptide, termed presenilin 1 (PS1), we document that the preponderant PS1-related species that accumulate in cultured mammalian cells, and in the brains of rodents, primates, and humans are approximately 27-28 kDa N-terminal and approximately 16-17 kDa C-terminal derivatives. Notably, a FAD-linked PS1 variant that lacks exon 9 is not subject to endoproteolytic cleavage. In brains of transgenic mice expressing human PS1, approximately 17 kDa and approximately 27 kDa PS1 derivatives accumulate to saturable levels, and at approximately 1:1 stoichiometry, independent of transgene-derived mRNA. We conclude that PS1 is subject to endoproteolytic processing in vivo.

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Citations

Cites

Show all 7 references

Research works printed but not linked: 25

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Metadata

Authors
Thinakaran, G., Borchelt, D. R., Lee, M. K., Slunt, H. H., Spitzer, L., Kim, G., Ratovitsky, T., Davenport, F., Nordstedt, C., Seeger, M., Hardy, J., Levey, A. I., Gandy, S. E., Jenkins, N. A., Copeland, N. G., Price, D. L., & Sisodia, S. S.
Venue
Neuron
Publisher
Elsevier BV
Published
1996-07
Volume
17
Issue
1
Pages
181-190
ISSN
0896-6273 (ISSN-L)
DOI
10.1016/s0896-6273(00)80291-3
OpenAlex
W2075175573
Cited by
1,013
References
7
FWCI
51.03
Type
journal article
Language
en
Open access
hybrid
Licence

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CoChat. (2026). Citation check: Endoproteolysis of Presenilin 1 and Accumulation of Processed Derivatives In Vivo. https://app.cochat.ai/papers/10.1016/s0896-6273(00)80291-3
The paper
Thinakaran, G., Borchelt, D. R., Lee, M. K., Slunt, H. H., Spitzer, L., Kim, G., Ratovitsky, T., Davenport, F., Nordstedt, C., Seeger, M., Hardy, J., Levey, A. I., Gandy, S. E., Jenkins, N. A., Copeland, N. G., Price, D. L., & Sisodia, S. S. (1996). Endoproteolysis of Presenilin 1 and Accumulation of Processed Derivatives In Vivo. Neuron. https://doi.org/10.1016/s0896-6273(00)80291-3

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.