Functional Properties of Ca2+-Inhibitable Type 5 and Type 6 Adenylyl Cyclases and Role of Ca2+ Increase in the Inhibition of Intracellular cAMP Content
Abstract
Among the different adenylyl cyclase (AC) isoforms, type 5 and type 6 constitute a subfamily which has the remarkable property of being inhibited by submicromolar Ca2+ concentrations in addition to Galphai-mediated processes. These independent and cumulative negative regulations are associated to a low basal enzymatic activity which can be strongly activated by Galphas-mediated interactions or forskolin. These properties ensure possible wide changes of cAMP synthesis. Regulation of cAMP synthesis by Ca2+ was studied in cultured or native cells which express naturally type 5 and/or type 6 AC, including well-defined renal epithelial cells. The results underline two characteristics of the inhibition due to agonist-elicited increase of intracellular Ca2+: i) Ca2+ rises achieved through capacitive Ca2+ entry or intracellular Ca2+ release can inhibit AC to a similar extent; and ii) in a same cell type, different agonists inducing similar overall Ca2+ rises elicit a variable inhibition of AC activity. The results suggest that a high efficiency of AC regulation by Ca2+ is linked to a requisite close localization of AC enzyme and Ca2+ rises.
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In hepatocytes, interactions between the V1a receptor and the glucagon receptor and their signalling pathways have been reported.
“Crosstalk between V1aR and glucagon receptor and their signalling pathways has been described also in hepatocytes [37,38].”
From Vasopressin and hydration play a major role in the development of glucose intolerance and hepatic steatosis in obese rats (2015) · cites this paper as [38]
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- Authors
- Chabardès, D., Imbert-Teboul, M., & Elalouf, J.-M.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- ORCID
- Elalouf, Jean-Marc
- Venue
- Cellular Signalling
- Publisher
- Elsevier BV
- Published
- 1999-09
- Volume
- 11
- Issue
- 9
- Pages
- 651-663
- ISSN
- 0898-6568 (ISSN-L)
- OpenAlex
- W2091166353
- Cited by
- 75
- References
- 94
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 1 · 2017: 3 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 5
- FWCI
- 0.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cellular Signalling (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phosphodiesterase function and regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- adenylyl cyclase type 5, calcium inhibition, intracellular cAMP, calcium signaling, store-operated calcium entry, intracellular calcium release, Gαi, Gs protein, forskolin, renal epithelial cells, enzyme regulation, cAMP production
- MeSH terms
- Adenylyl Cyclase Inhibitors, Cyclic AMP, Adenylyl Cyclases, Animals, Calcium, Humans, Intracellular Fluid, Isoenzymes
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