Cited by 75Protein Kinase Regulation and GTPase SignalingPhosphodiesterase function and regulationReceptor Mechanisms and Signaling

Functional Properties of Ca2+-Inhibitable Type 5 and Type 6 Adenylyl Cyclases and Role of Ca2+ Increase in the Inhibition of Intracellular cAMP Content

Chabardès, Danielle, Imbert-Teboul, Martine, Elalouf, Jean-Marc·1999·Cellular Signalling

Abstract

Among the different adenylyl cyclase (AC) isoforms, type 5 and type 6 constitute a subfamily which has the remarkable property of being inhibited by submicromolar Ca2+ concentrations in addition to Galphai-mediated processes. These independent and cumulative negative regulations are associated to a low basal enzymatic activity which can be strongly activated by Galphas-mediated interactions or forskolin. These properties ensure possible wide changes of cAMP synthesis. Regulation of cAMP synthesis by Ca2+ was studied in cultured or native cells which express naturally type 5 and/or type 6 AC, including well-defined renal epithelial cells. The results underline two characteristics of the inhibition due to agonist-elicited increase of intracellular Ca2+: i) Ca2+ rises achieved through capacitive Ca2+ entry or intracellular Ca2+ release can inhibit AC to a similar extent; and ii) in a same cell type, different agonists inducing similar overall Ca2+ rises elicit a variable inhibition of AC activity. The results suggest that a high efficiency of AC regulation by Ca2+ is linked to a requisite close localization of AC enzyme and Ca2+ rises.

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Crossref · 10.1016/s0898-6568(99)00031-5

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94 of 94 references verified0 citation errors · 0 not checkable

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Authors
Chabardès, D., Imbert-Teboul, M., & Elalouf, J.-M.
Affiliations
Centre National de la Recherche Scientifique (FR)
Venue
Cellular Signalling
Publisher
Elsevier BV
Published
1999-09
Volume
11
Issue
9
Pages
651-663
ISSN
0898-6568 (ISSN-L)
OpenAlex
W2091166353
Cited by
75
References
94
Citations by year
2026: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 1 · 2017: 3 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 5
FWCI
0.23
Type
journal article
Language
en
Open access
closed
Locations
  • Cellular Signalling (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phosphodiesterase function and regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
Keywords
adenylyl cyclase type 5, calcium inhibition, intracellular cAMP, calcium signaling, store-operated calcium entry, intracellular calcium release, Gαi, Gs protein, forskolin, renal epithelial cells, enzyme regulation, cAMP production
MeSH terms
Adenylyl Cyclase Inhibitors, Cyclic AMP, Adenylyl Cyclases, Animals, Calcium, Humans, Intracellular Fluid, Isoenzymes

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