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Cited by 363Receptor Mechanisms and SignalingProtein Kinase Regulation and GTPase SignalingPhosphodiesterase function and regulation

Type VIII adenylyl cyclase: a Ca2+/calmodulin-stimulated enzyme expressed in discrete regions of rat brain.

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Cali, J.J., Zwaagstra, J.C., Mons, N., Cooper, D.M., Krupinski, J.·1994·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(17)32700-x
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Abstract

A cDNA that encodes type VIII adenylyl cyclase has been isolated from two rat brain libraries. The open reading frame encodes a 1248-amino acid protein predicted to have two sets of six transmembrane spans and two putative nucleotide binding domains as is characteristic of other mammalian adenylyl cyclases. Two type VIII messages are detected in rat brain with estimated sizes of 5.5 and 4.4 kilobases. In situ hybridization indicates that the type VIII messages are most abundantly expressed in the granule cells of the dentate gyrus, the pyramidal cells of hippocampal fields CA1-CA3, the entorhinal cortex, and the piriform cortex. Hybridization is also detected in the neocortex, the amygdaloid complex, and regions of the thalamus and hypothalamus. Stable expression of the type VIII cDNA in human embryonal kidney cells leads to the appearance of a novel 165-kDa glycoprotein in the membrane fraction. Stimulation of these cells with agents that increase intracellular Ca2+ results in up to 43-fold increases in cAMP accumulation over that of control cells transfected with the expression vector. Addition of isoproterenol alone does not lead to type VIII-specific effects in intact cells. Adenylyl cyclase activity in membranes prepared from type VIII-transformed cells is stimulated up to 40-fold by the addition of Ca2+/calmodulin (EC50 = 53 nM calmodulin). The addition of activated recombinant alpha subunit of Gs synergistically increases the Ca2+/calmodulin-stimulated activity. A poss…

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  • AC8 is expressed in various areas of the hippocampus, including CA1-CA3 pyramidal cells as well as granule cells in the dentate gyrus.

    “AC8 also is expressed in various areas of the hippocampus, including CA1-CA3 pyramidal cells as well as granule cells in the dentate gyrus (Cali et al., 1994).”

    From Type I Adenylyl Cyclase Mutant Mice Have Impaired Mossy Fiber Long-Term Potentiation (1998) · cites this paper as Cali et al., 1994

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Authors
Cali, J. J., Zwaagstra, J. C., Mons, N., Cooper, D. M., & Krupinski, J.
Affiliations
Geisinger Medical Center (US)
ORCID
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1994-04
Volume
269
Issue
16
Pages
12190-12195
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(17)32700-x
OpenAlex
W1504397462
Cited by
363
References
5
Citations by year
2026: 3 · 2024: 1 · 2023: 3 · 2022: 6 · 2021: 7 · 2020: 4 · 2019: 3 · 2018: 5 · 2017: 6 · 2016: 3 · 2015: 4 · 2014: 8 · 2013: 6 · 2012: 12
FWCI
15.59
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
  • National Institutes of Health (5r29gm046395-03)
  • National Institute of General Medical Sciences (R01GM032483, GM 46395, GM 32483)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phosphodiesterase function and regulation
Keywords
cyclic AMP, rat brain, dentate gyrus granule cells, hippocampus, entorhinal cortex, piriform cortex, amygdala, thalamus, hypothalamus, cDNA cloning, transmembrane proteins, Gs alpha subunit, long-term potentiation, isoproterenol
MeSH terms
Cyclic AMP, Adenylyl Cyclases, Amino Acid Sequence, Animals, Base Sequence, Brain, Calcium, Calmodulin, Cell Line, Humans, Immunohistochemistry, Isoenzymes, Kidney, Kinetics, Male, Molecular Sequence Data, Organ Specificity, Peptides, Recombinant Proteins, Transfection, Protein Biosynthesis, Immunoblotting, Oligonucleotide Probes, Guanosine 5'-O-(3-Thiotriphosphate), Rats, Sprague-Dawley, In Situ Hybridization, DNA, Complementary, GTP-Binding Proteins, Rats

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