Cited by 30Estrogen and related hormone effectsCardiac electrophysiology and arrhythmiasIon channel regulation and function

Acute modulation of Ca2+ influx on rat heart by 17β-estradiol

Buitrago, Claudia, Massheimer, Virginia, de Boland, Ana Russo·2000·Cellular Signalling

Abstract

Estrogens initiate their action by binding to specific intracellular receptors and then acting on gene expression. In addition, there is growing evidence of a direct membrane effect via interaction with a cell surphase receptor. The aim of the present study was to investigate the acute effects of 17beta-estradiol on Ca2+ fluxes through second messenger pathways in rat cardiac muscle. Exposure of rat ventricle to low levels of 17beta-estradiol (10(-12)-10(-8) M) increased 45Ca2+ influx within 1 min (+38%); the response was biphasic, peaking at 2 and 5 min (+60 and +55%, respectively). The effect of the hormone on rat heart seems to be specific since 17alpha-estradiol, dihydrotestosterone, and progesterone were devoid of activity. The effect of 17beta-estradiol (5 min, 10(-10) M) was suppressed by nitrendipine (1 microM) and LaCl3 (10 microM), involving the activation of voltage-dependent Ca2+ channels in the acute increase of rat heart calcium influx by the hormone. 17Beta-estradiol rapidly increased cAMP content and PKA activity of rat cardiac muscle in parallel to the changes in Ca2+ uptake. In addition the cAMP antagonist Rp-cAMPS suppressed 17beta-estradiol-dependent Ca2+ influx. Altogether, the data suggest the involvement of the cAMP/PKA messenger system in the nongenomic modulation of Ca2+ influx in rat cardiac muscle by physiological levels of 17beta-estradiol.

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Crossref · 10.1016/s0898-6568(99)00066-2

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  • The bulk of studies evaluating sex differences in the AF-related risk of heart failure have showed no significant difference; however, a few studies have suggested higher risk in women.

    “The bulk of studies evaluating sex differences in the AF-related risk of HF have showed no significant difference; however, a few studies have suggested higher risk in women (Supplementary Table S8).”

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Authors
Buitrago, C., Massheimer, V., & de Boland, A. R.
Affiliations
Universidad Nacional del Sur (AR)
Venue
Cellular Signalling
Publisher
Elsevier BV
Published
2000-01
Volume
12
Issue
1
Pages
47-52
ISSN
0898-6568 (ISSN-L)
OpenAlex
W2045363416
Cited by
30
References
43
Citations by year
2026: 2 · 2024: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2016: 1 · 2015: 3 · 2012: 2
FWCI
1.55
Type
journal article
Language
en
Open access
closed
Locations
  • Cellular Signalling (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Keywords
estradiol, estrogen, calcium influx, myocardium, non-genomic estrogen signaling, voltage-gated calcium channels, cAMP/PKA signaling, second messenger pathways, membrane estrogen receptor, nitrendipine, lanthanum chloride, rat ventricle
MeSH terms
Animals, Calcium, Estradiol, Female, Myocardium, Signal Transduction, Ion Transport, Rats, Wistar, Rats, In Vitro Techniques

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