Characterization of npas3, a novel basic helix-loop-helix PAS gene expressed in the developing mouse nervous system
Open accessAbstract
Here we describe the cloning and expression pattern of a new bHLH-PAS domain gene, Npas3. Npas3 shares 50.2% amino acid sequence identity with Npas1 and a lesser similarity with other members of the bHLH-PAS domain family of transcription factors. Northern blot analysis detected Npas3 mRNA between 11.5 and 17.5 d.p.c. in embryonic development and exclusively in the adult brain. Whole-mount and section in situ hybridization assays revealed expression of Npas3 between 9.5 and 11.5 d.p.c. in the developing neural tube. In addition, Npas3 mRNA was expressed throughout the neuroepithelium of the developing central nervous system between 10. 5 and 12.5 d.p.c. Interestingly, at 14.5 d.p.c., the expression of Npas3 mRNA became restricted to the neopallial layer of the cortex. At 12.5 d.p.c., Npas3 mRNA was evident in nonneural tissues such as the developing dermis and mesenchyme surrounding the otic and nasal placodes. Expression was also detected in the developing cardiac valves, limb and developing kidney.
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NPAS3 encodes a bHLH-PAS transcription factor mainly expressed in the central nervous system of mice and humans, particularly in cortical developing interneurons, hippocampal neurons, and astrocytes.
“NPAS3 encodes a transcription factor of the bHLH-PAS family that is mainly expressed in the central nervous system of mice and humans, in particular in cortical developing interneurons, hippocampal neurons, and astrocytes (Brunskill et al. 1999; Gould and Kamnasaran 2011).”
From The Human Accelerated Region HAR202 Controls <i>NPAS3</i> Expression in the Developing Forebrain Displaying Differential Enhancer Activity Between Modern and Archaic Human Sequences (2024) · cites this paper as Brunskill et al. 1999
The Abstract identifies Npas3 as a bHLH-PAS gene and reports expression in the developing mouse CNS and adult brain. The Results describe expression in the developing cortical neopallial layer, but the paper does not establish expression in humans or identify cortical interneurons, hippocampal neurons, or astrocytes as expressing cells. (LLM, conf=0.88)
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“NPAS3 encodes a transcription factor of the bHLH-PAS family that is mainly expressed in the central nervous system of mice and humans, in particular in cortical developing interneurons, hippocampal neurons, and astrocytes (Brunskill et al. 1999; Gould and Kamnasaran 2011).”
- Authors
- Brunskill, E. W., Witte, D. P., Shreiner, A. B., & Potter, S. S.
- Affiliations
- Cincinnati Children's Hospital Medical Center (US)
- Corresponding author
- Potter, S.Steven
- Venue
- Mechanisms of Development
- Publisher
- Elsevier BV
- Published
- 1999-11
- Volume
- 88
- Issue
- 2
- Pages
- 237-241
- ISSN
- 0925-4773 (ISSN-L)
- OpenAlex
- W2039710055
- Cited by
- 64
- References
- 15
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 3 · 2022: 4 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 5 · 2017: 1 · 2016: 5 · 2015: 1 · 2014: 1 · 2013: 4 · 2012: 4
- FWCI
- 0.34
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Mechanisms of Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Heart Association
- National Heart, Lung, and Blood Institute (P01HL041496, HL41496)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
- Keywords
- NPAS3, bHLH transcription factors, PAS domain, gene cloning, gene expression patterns, neural tube development, neuroepithelium, neocortex, embryonic development, placodes, heart valves, limb development, kidney development, mouse embryo
- MeSH terms
- Amino Acid Sequence, Animals, Brain, Epithelium, Mesoderm, Molecular Sequence Data, Nerve Tissue Proteins, Nervous System, Transcription Factors, Blotting, Northern, Sequence Homology, Amino Acid, In Situ Hybridization, Helix-Loop-Helix Motifs, Gene Expression Regulation, Developmental, Expressed Sequence Tags, Mice, Basic Helix-Loop-Helix Proteins
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