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Role of exopolysaccharides produced by Lactococcus lactis subsp. cremoris on the viscosity of fermented milks
Abstract
Lactic acid bacteria strains that produce exopolysaccharide (EPS) are employed in the manufacture of fermented milk to improve its texture and viscosity. No clear correlation has been found between EPS concentration and the viscosity of the fermented milk. It is supposed that the molecular characteristics, i.e. radius of gyration and molar mass, of these biopolymers play a part. The subject of this study was to clarify whether the viscosifying properties of EPS in aqueous solution can be related to the viscosity of fermented milk products. Therefore, we aimed at relating the molecular characteristics of EPSs produced by several strains of Lactococcus lactis subsp. cremoris in milk at three incubation temperatures to the viscosity of the fermented products. For a given EPS concentration, a positive correlation between the EPS-viscosifying parameters (intrinsic viscosity, Kuhn segments length and thickening efficiency) and the Posthumus viscosity of fermented milks was found.
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“On the other hand, a study by Ruas-Madiedo et al. [70] reported no effect of temperature on the EPS production when the Lact. lactis subsp. cremoris strain was used to ferment milk.”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [70]
In the citing paper
“As a preceding step to EPS purification, membrane filtration techniques such as microfiltration, ultrafiltration and/or diafiltration have also been used [50,58,70,101].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [70]
In the citing paper
“Then, proteins can be precipitated with trichloroacetic acid (TCA) [27,49,58,70] or a combination of TCA and proteases [97].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [70]
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Nguyen, A. T.-B., Nigen, M., Jimenez, L., Ait-Abderrahim, H., Cunault, C., Marchesseau, S., & Picart‐Palmade, L. (2018) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2018.07.016 · Cited by 21
The physicochemical and sensory evaluation of commercial sour milk (amasi) products
Moyane, N. (2013) · African Journal of Food Science · doi:10.5897/ajfs12.089 · Cited by 20
“The LAB that produce exopoly- saccharides are often used to increase the viscosity of stirred fermented milks, such as yoghurt and to decrease the susceptibility to syneresis (Ruas-Madiedo et al., 2002).”
Nguyen, A. T.-B., Nigen, M., Jimenez, L., Ait-Abderrahim, H., Marchesseau, S., & Picart‐Palmade, L. (2017) · Food Chemistry · doi:10.1016/j.foodchem.2017.06.121 · Cited by 19
Burns, P., Vinderola, G., Reinheimer, J. A., Cuesta, I., Reyes‐Gavilán, C. G. D. L., & Ruas‐Madiedo, P. (2011) · Journal of Dairy Research · doi:10.1017/s0022029911000355 · Cited by 20
“It has been indicated that linkage type into the backbone of repeating unit building HePS, as well as the presence of side chains or the branching degree, among others, are parameters contributing to the viscosity intensifying ability of these polymers in fermented milks (Ruas-Madiedo et al. 2002).”
BackgroundResultDeveloping new polysaccharides
Vuyst, L. D., & Vaningelgem, F. (2003) · Elsevier eBooks · doi:10.1533/9781855737082.2.275 · Cited by 20
Physicochemical characterisation of the exopolysaccharides of Streptococcus thermophilus ST ‐143
Mende, S., Dong, T., Rathemacher, A., Rohm, H., & Jaros, D. (2014) · International Journal of Food Science & Technology · doi:10.1111/ijfs.12505 · Cited by 18
Kılıç, E. E., Kılıç, İ. H., & Koç, B. (2022) · Journal of Chemistry · doi:10.1155/2022/2683126 · Cited by 16
Liu, L., Li, C., & Liu, J. (2016) · International Journal of Food Properties · doi:10.1080/10942912.2016.1180531 · Cited by 16
Priyashantha, H., Buldo, P., Berg, T., Gilleladen, C., & Ipsen, R. (2021) · Food Structure · doi:10.1016/j.foostr.2021.100232 · Cited by 14
Priyashantha, H., Quintáns, A. P., Baixauli, R., & Vidanarachchi, J. K. (2019) · Journal of Texture Studies · doi:10.1111/jtxs.12449 · Cited by 14
Idoui, T., & Karam, N.-E. (2008) · Grasas y Aceites · doi:10.3989/gya.2008.v59.i4.530 · Cited by 14
“The presence of viscous extracellular polysaccharides produced by some culture bacteria was also shown to play an important role in achieving satisfactory firmness and apparent viscosity in yoghurt (Patricia et al., 2002).”
BackgroundEffect of incubation temperature and caseinates on the rheological behaviour of Kefir
Dimitreli, G., & Antoniou, K. D. (2011) · Procedia Food Science · doi:10.1016/j.profoo.2011.09.088 · Cited by 13
“Furthermore, their production is closely related with the optimal growth conditions of the bacteria that produce them [12].”
BackgroundZhang, S., & Zhang, L. (2012) · Advanced materials research · doi:10.4028/www.scientific.net/amr.430-432.890 · Cited by 13
Metadata
- Authors
- Ruas-Madiedo, P., Tuinier, R., Kanning, M., & Zoon, P.
- Affiliations
- Nederlands Instituut Voor Zuivel Oonderzoek (NL)
- Corresponding author
- Zoon, Pieternella
- ORCID
- Tuinier, Remco
- Venue
- International Dairy Journal
- Publisher
- Elsevier BV
- Published
- 2002
- Volume
- 12
- Issue
- 8
- Pages
- 689-695
- ISSN
- 0958-6946 (ISSN-L)
- OpenAlex
- W2104371637
- Cited by
- 152
- References
- 34
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1 · 2023: 4 · 2022: 2 · 2021: 8 · 2020: 5 · 2019: 9 · 2018: 4 · 2017: 10 · 2016: 10 · 2015: 10 · 2014: 8 · 2013: 8 · 2012: 10
- FWCI
- 12.44
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Dairy Journal (publishedVersion)
- TU/e Research Portal (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Keywords
- exopolysaccharides, Lactococcus lactis subsp. cremoris, fermented milk, viscosity, intrinsic viscosity, Kuhn segment length, radius of gyration, molecular weight, incubation temperature, lactic acid bacteria
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