Cited by 410Protein Kinase Regulation and GTPase SignalingReceptor Mechanisms and SignalingCellular transport and secretion

GTPase-activating proteins: Helping hands to complement an active site

Scheffzek, Klaus, Ahmadian, Mohammad Reza, Wittinghofer, Alfred·1998·Trends in Biochemical Sciences

Abstract

Stimulation of the intrinsic GTPase activity of GTP-binding proteins by GTPase-activating proteins (GAPs) is a basic principle of GTP-binding-protein downregulation. Recently, the molecular mechanism behind this reaction has been elucidated by studies on Ras and Rho, and their respective GAPs. The basic features involve stabilizing the existing catalytic machinery and supplementing it by an external arginine residue. This represents a novel mechanism for enzyme active-site formation.

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Crossref · 10.1016/s0968-0004(98)01224-9

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57 of 57 references verified0 citation errors · 0 not checkable

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Authors
Scheffzek, K., Ahmadian, M. R., & Wittinghofer, A.
Affiliations
Max Planck Society (DE)
Venue
Trends in Biochemical Sciences
Publisher
Elsevier BV
Published
1998-07
Volume
23
Issue
7
Pages
257-262
ISSN
0968-0004 (ISSN-L)
OpenAlex
W1973728462
Cited by
410
References
57
Citations by year
2026: 3 · 2025: 4 · 2024: 2 · 2023: 6 · 2022: 6 · 2021: 10 · 2020: 5 · 2019: 3 · 2018: 7 · 2017: 7 · 2016: 9 · 2015: 6 · 2014: 11 · 2013: 9 · 2012: 20
FWCI
2.88
Type
journal article
Language
en
Open access
closed
Locations
  • Trends in Biochemical Sciences (publishedVersion)
  • PubMed (publishedVersion)
  • Max Planck Institute for Plasma Physics (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords
GTPase-activating protein, G proteins, GTPase activity, Ras, Rho, active site formation, arginine residues, enzyme catalysis, protein downregulation
MeSH terms
Amino Acid Sequence, Animals, Binding Sites, Enzyme Activation, Humans, Models, Biological, Models, Molecular, Protein Conformation, Proteins, Sequence Homology, Amino Acid, GTP Phosphohydrolases, GTPase-Activating Proteins, ras GTPase-Activating Proteins

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