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An open and closed case for all polymerases
Open accessAbstract
The recently determined structures of HIV-1 reverse transcriptase and Taq DNA polymerase in complex with DNA primer-template and an incoming nucleotide have shown that a large conformational change configures the polymerase active site for nucleotidyl transfer. The structure of reverse transcriptase in the catalytic complex will open the path to the rational design of novel nucleoside analog inhibitors of viral replication.
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“Crystal structures (Refs.15-22 and references therein) reveal that polymerase binding to DNA strongly reshapes the primertemplate, e.g. the backbone at the templating base can be bent by 90°.”
From DNA Replication Fidelity (2004) · cites this paper as [18]
In the citing paper
“Polymerases in families A, B, X, and RT have nascent base pair binding pockets that tightly accommodate a correct Watson-Crick base pair (Fig. 2A and additional images in Refs.15-19 and references therein).”
From DNA Replication Fidelity (2004) · cites this paper as [18]
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Bumps in the road: how replicative DNA polymerases see DNA damage
Hogg, M., Wallace, S. S., & Doublié, S. (2005) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2005.01.014 · Cited by 58
Self-citation
Metadata
- Authors
- Doublié, S., Sawaya, M. R., & Ellenberger, T.
- Affiliations
- University of Vermont (US)
- Harvard University (US)
- Corresponding author
- Ellenberger, Tom
- Venue
- Structure
- Publisher
- Elsevier BV
- Published
- 1999-02
- Volume
- 7
- Issue
- 2
- Pages
- R31-R35
- ISSN
- 0969-2126 (ISSN-L)
- OpenAlex
- W2036894316
- Cited by
- 314
- References
- 24
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 8 · 2023: 5 · 2022: 6 · 2021: 7 · 2020: 6 · 2019: 4 · 2018: 6 · 2017: 11 · 2016: 9 · 2015: 8 · 2014: 19 · 2013: 13 · 2012: 8
- FWCI
- 13.72
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Structure (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- polymerase active site, HIV-1 reverse transcriptase, Taq DNA polymerase, nucleotidyl transfer, conformational change, primer-template complex, rational drug design, polymerase structure
- MeSH terms
- Binding Sites, DNA-Directed DNA Polymerase, Models, Molecular, HIV-1, Protein Structure, Secondary, DNA Primers, Taq Polymerase, HIV Reverse Transcriptase
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