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Fragmentation of phosphopeptides in an ion trap mass spectrometer
Abstract
A systematic study of the fragmentation pattern of phosphopeptides in an electrospray (ESI) ion trap mass spectrometer is presented. We show that phosphotyrosine- and phosphothreonine-containing peptides show complicated fragmentation patterns. These phosphopeptides were observed to lose the phosphate moiety in the form of H3PO4 and/or HPO3, but were also detected with no loss of the phosphate group. The tendency to lose the phosphate moiety depends strongly on the charge state. Thus, the highest observed charge state tends to retain the phosphate moiety with extensive fragmentation along the peptide backbone. We also show that phosphoserine-containing peptides have relatively simple fragmentation patterns of losing H3PO4. This loss is independent of the charge state. We suggest strategies for the accurate identification of phosphorylation sites using the ion trap mass spectrometer.
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Citations
Cited by
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The first identification of lysine malonylation substrates and its regulatory enzyme
Peng, C., Lu, Z., Xie, Z., Cheng, Z., Chen, Y., Tan, M., Luo, H., Zhang, Y., He, W., Yang, K., et al. (2011) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m111.012658 · Cited by 751
InsPecT: Identification of Posttranslationally Modified Peptides from Tandem Mass Spectra
Tanner, S., Shu, H., Frank, A., Wang, L.-C., Zandi, E., Mumby, M., Pevzner, P. A., & Bafna, V. (2005) · Analytical Chemistry · doi:10.1021/ac050102d · Cited by 567
Zarling, A. L., Ficarro, S. B., White, F. M., Shabanowitz, J., Hunt, D. F., & Engelhard, V. H. (2000) · The Journal of Experimental Medicine · doi:10.1084/jem.192.12.1755 · Cited by 213
Metadata
- Authors
- DeGnore, J. P., & Qin, J.
- Venue
- Journal of the American Society for Mass Spectrometry
- Publisher
- American Chemical Society (ACS)
- Published
- 1998-11-01
- Volume
- 9
- Issue
- 11
- Pages
- 1175-1188
- ISSN
- 1044-0305 (ISSN-L)
- OpenAlex
- W2002609852
- Cited by
- 257
- References
- 0
- FWCI
- 4.29
- Type
- journal article
- Language
- en
- Open access
- closed
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- DeGnore, J. P., & Qin, J. (1998). Fragmentation of phosphopeptides in an ion trap mass spectrometer. Journal of the American Society for Mass Spectrometry. https://doi.org/10.1016/s1044-0305(98)00088-9
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