The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
The effect of storage at different temperatures on the stability of Hepatitis C virus RNA in plasma samples
Abstract
One important issue related to Hepatitis C virus (HCV) RNA nucleic acid amplification testing (NAT) is the storage conditions of plasma samples in order to obtain reliable results. Many authors have reported that the storage conditions could affect the RNA stability and, hence, HCV RNA detection. We have studied HCV RNA stability in plasma samples after storage at different temperatures (-70, -20, 5 and 25 degrees C). Samples containing different HCV titres were stored and analysed by qualitative or quantitative NAT techniques at defined time points. At -20 degrees C, samples containing high HCV RNA titres were followed-up during approximately 2.6-2.7 years, samples with intermediate concentrations during approximately 1 year and samples with 100 International Units/millilitre (IU/ml) during 2.5 years. Independently of the HCV RNA concentration, the results show absence of decay in HCV RNA detectability. Samples stored at 25 degrees C maintain their HCV RNA titre during 14 days and samples at 5 degrees C were stable for at least 3 months.
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CSF samples stored at −80 °C for up to five years did not significantly impact viral load.
“Regarding the stability of CSF samples stored at −80 °C for up to five years, although RNA is generally more prone to degradation than double-stranded DNA, we do not believe this storage condition significantly impacted the viral load.”
From Performance Evaluation of Multiplex Molecular Syndromic Panel vs. Singleplex PCR for Diagnosis of Acute Central Nervous System Infections (2025) · cites this paper as [22]
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Measuring Influenza RNA Quantity after Prolonged Storage or Multiple Freeze/Thaw Cycles
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Metadata
- Authors
- José, M., Curtu, S., Gajardo, R., & Jorquera, J. I.
- Affiliations
- Grifols (Spain) (ES)
- Corresponding author
- José, Marta
- Venue
- Biologicals
- Publisher
- Elsevier BV
- Published
- 2003-03
- Volume
- 31
- Issue
- 1
- Pages
- 1-8
- ISSN
- 1045-1056 (ISSN-L)
- OpenAlex
- W2014441326
- Cited by
- 25
- References
- 22
- Citations by year
- 2025: 3 · 2023: 2 · 2020: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 0.43
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biologicals (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hepatology › Hepatitis C virus research
- Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- hepatitis C virus, storage temperature, nucleic acid amplification tests, RNA degradation, long-term storage
- MeSH terms
- Humans, RNA, Viral, Specimen Handling, Temperature, Hepacivirus
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