The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Measuring influenza RNA quantity after prolonged storage or multiple freeze/thaw cycles
Abstract
In this study, we aim to determine what effects prolonged storage and repeated freeze/thaw cycles have on the stability of influenza A(H1N1)pdm09 (influenza A/H1N1)RNA. Cloned influenza A/H1N1 RNA transcripts were serially diluted from 8.0-1.0 log 10 copies/μl. RT-qPCR was used to measure RNA loss in transcripts stored at -80°C, -20°C, 4°C and 25°C for up to 84days or transcripts undergoing a total of 10 freeze/thaw cycles. Viral load was measured in clinical specimens stored at-80°C for three years (n=89 influenza A RNA extracts; n=35 primary specimens) and in 10 clinical specimens from the 2015/2016 influenza season that underwent 7 freeze/thaw cycles. RNA stored at -80°C, -20°C, 4°C and 25°C is stable for up to 56, 56, 21, and 7days respectively or up to 9 freeze/thaw cycles when stored at -80°C. There is no difference in viral load in clinical specimens that have been stored for up to three years at -80°C if they are re-extracted. Similarly, clinical specimens undergoing up to 7 freeze/thaw cycles are stable if they are re-extracted between cycles. Influenza specimens can be stored for up to three years at -80°C or undergo up to 7 freeze/thaw cycles without loss of RNA quantity if re-extracted.
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CSF samples stored at −80 °C for up to five years did not significantly impact viral load.
“Regarding the stability of CSF samples stored at −80 °C for up to five years, although RNA is generally more prone to degradation than double-stranded DNA, we do not believe this storage condition significantly impacted the viral load.”
From Performance Evaluation of Multiplex Molecular Syndromic Panel vs. Singleplex PCR for Diagnosis of Acute Central Nervous System Infections (2025) · cites this paper as [21]
Stability studies on influenza virus demonstrated no difference in viral load when clinical specimens were stored at −80 °C for up to three years and re-extracted.
“Stability studies on influenza virus by Granados et al. [21] have demonstrated no difference in viral load when clinical specimens were stored at −80 °C for up to three years and re-extracted.”
From Performance Evaluation of Multiplex Molecular Syndromic Panel vs. Singleplex PCR for Diagnosis of Acute Central Nervous System Infections (2025) · cites this paper as Granados et al. [21]
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Cites
Jerome, K. R., Huang, M.-L. W., Wald, A., Selke, S., & Corey, L. (2002) · Journal of Clinical Microbiology · doi:10.1128/jcm.40.7.2609-2611.2002 · Cited by 155
Influenza and rhinovirus viral load and disease severity in upper respiratory tract infections
Granados, A., Peci, A., McGeer, A., et al. (2016) · Journal of Clinical Virology · doi:10.1016/j.jcv.2016.11.008 · Cited by 103
Self-citationKrafft, A. E., Russell, K. L., Hawksworth, A. W., McCall, S. A., Irvine, M., Daum, L. T., Connoly, J. L., Reid, A. H., Gaydos, J. C., & Taubenberger, J. K. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.4.1768-1775.2005 · Cited by 73
Krajden, M., Minor, J. M., Rifkin, O., et al. (1999) · Journal of Clinical Microbiology · doi:10.1128/jcm.37.6.1683-1686.1999 · Cited by 68
Stability of HCV, HIV-1 and HBV nucleic acids in plasma samples under long-term storage
José, M., Gajardo, R., & Jorquera, J. I. (2005) · Biologicals · doi:10.1016/j.biologicals.2004.10.003 · Cited by 48
Bellete, B., Flori, P., Hafid, J. E., et al. (2003) · Journal of Microbiological Methods · doi:10.1016/s0167-7012(03)00141-6 · Cited by 47
Stability of hepatitis C virus RNA during specimen handling and storage prior to NASBA amplification
Damen, M., Sillekens, P. T. G., Sjerps, M., et al. (1998) · Journal of Virological Methods · doi:10.1016/s0166-0934(98)00024-x · Cited by 38
Frisbie, B., Tang, Y., Griffin, M. R., et al. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.3.1181-1184.2004 · Cited by 32
A Universal Influenza A and B Duplex Real‐time RT‐PCR Assay
Lee, H. K., Loh, T. P., Lee, C. K., et al. (2012) · Journal of Medical Virology · doi:10.1002/jmv.23375 · Cited by 29
Dauner, A. L., Gilliland, T. C., Mitra, I., Pal, S., Morrison, A. C., Hontz, R. D., & Wu, S.-J. L. (2015) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.15-0110 · Cited by 28
José, M., Curtu, S., Gajardo, R., & Jorquera, J. I. (2003) · Biologicals · doi:10.1016/s1045-1056(02)00067-2 · Cited by 25
Cömert, F., Aktaş, E., Terzi, H. A., et al. (2012) · Diagnostic Microbiology and Infectious Disease · doi:10.1016/j.diagmicrobio.2012.09.017 · Cited by 17
Viral Quantitative Capillary Electrophoresis for Counting and Quality Control of RNA Viruses
Azizi, A., Mironov, G. G., Muharemagic, D., et al. (2012) · Analytical Chemistry · doi:10.1021/ac302525y · Cited by 17
Wang, X. B., Zoueva, O., Zhao, J., et al. (2011) · BMC Infectious Diseases · doi:10.1186/1471-2334-11-354 · Cited by 16
Dare, R. K., Zhu, Y., Williams, J. V., et al. (2016) · Journal of Medical Virology · doi:10.1002/jmv.24549 · Cited by 13
Granados, A., Quach, S., McGeer, A., et al. (2016) · Journal of Medical Virology · doi:10.1002/jmv.24753 · Cited by 12
Self-citationUse of dried clinical samples for storing and detecting influenza RNA
Winters, M. A., Lloyd, R., Jr., Shahidi, A., et al. (2011) · Influenza and Other Respiratory Viruses · doi:10.1111/j.1750-2659.2011.00253.x · Cited by 10
Stability of lyophilised specimens for the molecular detection of viral DNA/RNA
Vaughan, H., Chalker, V. J., Mee, Z., et al. (2005) · Journal of Clinical Virology · doi:10.1016/j.jcv.2005.06.001 · Cited by 9
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Gabrielli, L., Tomaiuolo, M., Banchini, I., Balboni, A., Liberatore, A., Lanna, F., Cantiani, A., Bertoldi, A., Pavoni, M., Manzoli, L., et al. (2025) · Microorganisms · doi:10.3390/microorganisms13040892 · Cited by 5
“Stability studies on influenza virus by Granados et al. [21] have demonstrated no difference in viral load when clinical specimens were stored at − 80 ◦ C for up to three years and re-extracted.”
Metadata
- Authors
- Granados, A., Petrich, A., McGeer, A., & Gubbay, J. B.
- Affiliations
- University of Toronto (CA)
- Corresponding author
- Granados, Andrea
- Venue
- Journal of Virological Methods
- Publisher
- Elsevier BV
- Published
- 2017-09
- Volume
- 247
- Pages
- 45-50
- ISSN
- 0166-0934 (ISSN-L)
- OpenAlex
- W2618357101
- Cited by
- 23
- References
- 18
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 5 · 2020: 4 · 2019: 3 · 2018: 1
- FWCI
- 1.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Virological Methods (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
- Keywords
- influenza A(H1N1)pdm09, RNA stability, RNA storage, freeze-thaw cycles, RNA degradation, viral load, clinical samples, long-term sample storage, RT-qPCR
- MeSH terms
- Freezing, Humans, Preservation, Biological, RNA, Viral, Specimen Handling, Viral Load, Reverse Transcriptase Polymerase Chain Reaction, Genomic Instability, Influenza A Virus, H1N1 Subtype, Real-Time Polymerase Chain Reaction
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