Cited by 164Ubiquitin and proteasome pathwaysProtein Degradation and InhibitorsAutophagy in Disease and Therapy

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Harnessing the Ubiquitination Machinery to Target the Degradation of Specific Cellular Proteins

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Zhou, Pengbo, Bogacki, Robert, McReynolds, Lisa, Howley, Peter M.·2000·Molecular Cell

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Abstract

The functional characterization of a specific gene, or its protein product, often relies on assessing the consequences of its elimination, usually accomplished by gene knockout, ribozyme, antisense, or RNA-mediated interference (RNAi) technologies. The selective degradation of cellular proteins is mediated primarily by the ubiquitin-proteasome pathway. Manipulation of the ubiquitin-dependent proteolytic machinery to eliminate specific gene products at the protein level has been previously attempted with some success in vitro; however, the in vivo efficacy of this approach has not yet been achieved. Here we report successful engineering of the substrate receptor of a major ubiquitin-proteolytic machinery to direct the degradation of otherwise stable cellular proteins both in yeast and in mammalian cells.

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Authors: Zhou, P., Bogacki, R., McReynolds, L., & Howley, P. M.
Affiliations: Harvard University (US)
Corresponding author: Zhou, Pengbo
Howley, Peter M.
ORCID: Zhou, Pengbo
McReynolds, Lisa
Howley, Peter M.
Venue: Molecular Cell
Publisher: Elsevier BV
Published: 2000-09
Volume: 6
Issue: 3
Pages: 751-756
ISSN: 1097-2765 (ISSN-L)
DOI: 10.1016/s1097-2765(00)00074-5
OpenAlex: W2034414781
Cited by: 164
References: 23
Citations by year: 2026: 1 · 2025: 7 · 2024: 7 · 2023: 9 · 2022: 8 · 2021: 3 · 2020: 8 · 2019: 6 · 2018: 5 · 2017: 5 · 2016: 7 · 2015: 6 · 2014: 9 · 2013: 6 · 2012: 2
FWCI: 2.65
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 4702 d)
Locations: Molecular Cell (publishedVersion)
PubMed (publishedVersion)
Funders: National Cancer Institute (R01CA064888, R01-CA64888-06)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Degradation and Inhibitors
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
Keywords: ubiquitination, protein degradation, ubiquitin-proteasome system, selective protein degradation, proteasome, RNA interference, yeast, mammalian cells
MeSH terms: F-Box-WD Repeat-Containing Protein 7, Animals, Uterine Cervical Neoplasms, Female, Humans, Ligases, Mammals, Molecular Biology, Nuclear Proteins, Plasmids, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Osteosarcoma, Substrate Specificity, Tumor Cells, Cultured, Ubiquitins, Gene Expression Regulation, Enzymologic, Retinoblastoma Protein, Cell Cycle Proteins, GTP-Binding Proteins, Saccharomyces cerevisiae Proteins, Ubiquitin-Protein Ligases, F-Box Proteins, beta-Transducin Repeat-Containing Proteins, Retinoblastoma-Like Protein p107

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Harnessing the Ubiquitination Machinery to Target the Degradation of Specific Cellular Proteins

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