CoChat
Sign Up
Cited by 160

The DOI matches its Crossref record, with a small difference in title or year; 3 of the 3 references it cites were found in a registry or an index.

Modulation of Host Signaling by a Bacterial Mimic

Stebbins, C.Erec, Galán, Jorge E·2000·Molecular Cell
DOI 10.1016/s1097-2765(00)00141-6
Open this paper in CoChat — free account

Abstract

Salmonella spp. utilize a specialized protein secretion system to deliver a battery of effector proteins into host cells. Several of these effectors stimulate Cdc42- and Rac1-dependent cytoskeletal changes that promote bacterial internalization. These potentially cytotoxic alterations are rapidly reversed by the effector SptP, a tyrosine phosphatase and GTPase activating protein (GAP) that targets Cdc42 and Rac1. The 2.3 A resolution crystal structure of an SptP-Rac1 transition state complex reveals an unusual GAP architecture that mimics host functional homologs. The phosphatase domain possesses a conserved active site but distinct surface properties. Binding to Rac1 induces a dramatic stabilization in SptP of a four-helix bundle that makes extensive contacts with the Switch I and Switch II regions of the GTPase.

Overview

Integrity signals

Registry

Crossref · 10.1016/s1097-2765(00)00141-6

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 3 references

Research works printed but not linked: 42

  1. Abrahams, 1996, Methods used in the structure determination of bovine mitochondiral F1 ATPase, Acta Crystallogr. D, 52, 30, 10.1107/S0907444995008754 · DOI
  2. Barford, 1994, Crystal structure of human protein tyrosine phosphatase 1B, Science, 263, 1397, 10.1126/science.8128219 · DOI
  3. Barrett, 1997, The structure of the GTPase-activating domain from p50rhoGAP, Nature, 385, 458, 10.1038/385458a0 · DOI
  4. Berger, 1995, Predicting coiled coils by use of pairwise residue correlations, Proc. Natl. Acad. Sci. USA, 92, 8259, 10.1073/pnas.92.18.8259 · DOI
  5. Black, 1997, Identification of p130Cas as a substrate of Yersinia YopH (Yop51), a bacterial protein tyrosine phosphatase that translocates into mammalian cells and targets focal adhesions, EMBO J., 16, 2730, 10.1093/emboj/16.10.2730 · DOI
  6. Bolin, 1988, The plasmid-encoded Yop2b protein of Yersinia pseudotuberculosis is a virulence determinant regulated by calcium and temperature at the level of transcription, Mol. Microbiol., 2, 237, 10.1111/j.1365-2958.1988.tb00025.x · DOI
  7. Brunger, 1998, Crystallography & NMR system, Acta Crystallogr. D Biol. Crystallogr., 54, 905, 10.1107/S0907444998003254 · DOI
  8. Coleman, 1994, Structures of active conformations of Gi alpha 1 and the mechanism of GTP hydrolysis, Science, 265, 1405, 10.1126/science.8073283 · DOI
  9. de La Fortelle, 1997, Maximum-likelihood heavy-atom parameter refinement in the MIR and MAD methods, Methods Enzymol., 276, 472, 10.1016/S0076-6879(97)76073-7 · DOI
  10. Finlay, 1997, Common themes in microbial pathogenicity revisited, Microbiol. Mol. Biol. Rev., 61, 136, 10.1128/.61.2.136-169.1997 · DOI
  11. Fiorentini, 1998, Bacterial toxins and the Rho GTP-binding protein, Cell Death Differ, 5, 720, 10.1038/sj.cdd.4400412 · DOI
  12. Forsberg, 1990, Genetic analysis of the yopE region of Yersinia spp., J. Bacteriol., 172, 1547, 10.1128/jb.172.3.1547-1555.1990 · DOI
  13. Fu, 1999, A Salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasion, Nature, 401, 293, 10.1038/45829 · DOI
  14. Galan, 1999, Interaction of Salmonella with host cells through the centisome 63 type III secretion system, Curr. Opin. Microbiol., 2, 46, 10.1016/S1369-5274(99)80008-3 · DOI
  15. Galan, 1996, Cross-talk between bacterial pathogens and their host cells, Annu. Rev. Cell Dev. Biol., 12, 219, 10.1146/annurev.cellbio.12.1.221 · DOI
  16. Galan, 1999, Type III secretion machines, Science, 284, 322, 10.1126/science.284.5418.1322 · DOI
  17. Goehring, 1999, The N-terminal domain of Pseudomonas aeruginosa exoenzyme S is a GTPase-activating protein for Rho GTPases, J. Biol. Chem., 274, 36369, 10.1074/jbc.274.51.36369 · DOI
  18. Guex, 1997, SWISS-MODEL and the Swiss-Pdb viewer, Electrophoresis, 18, 2714, 10.1002/elps.1150181505 · DOI
  19. Hall, 1998, Rho GTPases and the actin cytoskeleton, Science, 279, 509, 10.1126/science.279.5350.509 · DOI
  20. Hardt, 1998, Salmonella typhimurium encodes an activator of Rho GTPases that induces membrane ruffling and nuclear responses in host cells, Cell, 93, 815, 10.1016/S0092-8674(00)81442-7 · DOI
  21. Hirshberg, 1997, The crystal structure of human rac1, a member of the rho-family complexed with a GTP analogue, Nat. Struct. Biol., 4, 147, 10.1038/nsb0297-147 · DOI
  22. Jones, 1991, Improved methods for binding protein models in electron density maps and the location of errors in these models, Acta Crystallogr. A, 47, 110, 10.1107/S0108767390010224 · DOI
  23. Kaniga, 1996, A secreted tyrosine phosphatase with modular effector domains encoded by the bacterial pathogen Salmonella typhimurium, Mol. Microbiol., 21, 633, 10.1111/j.1365-2958.1996.tb02571.x · DOI
  24. Kraulis, 1991, Molscript, J. Appl. Crystallogr., 24, 946, 10.1107/S0021889891004399 · DOI
  25. Kulich, 1994, Cloning the structural gene for the 49-kDa form of exoenzyme S (exoS) from Pseudomonas aeruginosa strain 388, J. Biol. Chem., 269, 10431, 10.1016/S0021-9258(17)34078-4 · DOI
  26. Laskowski, 1998, Validation of protein models derived from experiment, Curr. Opin. Struct. Biol., 8, 631, 10.1016/S0959-440X(98)80156-5 · DOI
  27. Merrit, 1994, Raster3D Version 2.0, Acta Crystallogr. D, 50, 869, 10.1107/S0907444994006396 · DOI
  28. Michiels, 1988, Nucleotide sequence and transcription analysis of yop51 from Yersinia enterocolitica W22703, Microb. Pathog., 5, 449, 10.1016/0882-4010(88)90006-X · DOI
  29. Michiels, 1990, Secretion of Yop proteins by Yersiniae, Infect. Immun., 58, 2840, 10.1128/IAI.58.9.2840-2849.1990 · DOI
  30. Nassar, 1998, Structures of Cdc42 bound to the active and catalytically compromised forms of Cdc42GAP, Nat. Struct. Biol., 5, 1047, 10.1038/4156 · DOI
  31. Otwinowski, 1997, Processing of X-ray diffraction data collected in oscillation mode, Methods Enzymol., 276, 307, 10.1016/S0076-6879(97)76066-X · DOI · Paper page
  32. Pang, 1998, Typhoid fever-important issues still remain, Trends Microbiol., 6, 131, 10.1016/S0966-842X(98)01236-0 · DOI
  33. Persson, 1997, The PTPase YopH inhibits uptake of Yersinia, tyrosine phosphorylation of p130Cas and FAK, and the associated accumulation of these proteins in peripheral focal adhesions, EMBO J., 16, 2307, 10.1093/emboj/16.9.2307 · DOI
  34. Rittinger, 1997, Structure at 1.65 A of RhoA and its GTPase-activating protein in complex with a transition-state analogue, Nature, 389, 758, 10.1038/39651 · DOI
  35. Self, 1995, Measurements of intrinsic nucleotide exchange and GTP hydrolysis rates, Meth. Enzymol., 256, 67, 10.1016/0076-6879(95)56010-6 · DOI
  36. Sondek, 1994, GTPase mechanism of G proteins from the 1.7-A crystal structure of transducin alpha-GDP-AIF-4, Nature, 372, 276, 10.1038/372276a0 · DOI
  37. Stender, 2000, Identification of SopE2 from Salmonella typhimurium, a conserved guanine nucleotide exchange factor for Cdc42 of the host cell, Mol. Microbiol., 36, 1206, 10.1046/j.1365-2958.2000.01933.x · DOI
  38. Stuckey, 1994, Crystal structure of Yersinia protein tyrosine phosphatase at 2.5 A and the complex with tungstate, Nature, 370, 571, 10.1038/370571a0 · DOI
  39. Takeuchi, 1967, Electron microscopic studies of experimental Salmonella infection. 1. Penetration into the intestinal epithelium by Salmonella typhimurium, Am. J. Pathol., 50, 109
  40. Van Aelst, 1997, Rho GTPases and signaling networks, Genes Dev., 11, 2295, 10.1101/gad.11.18.2295 · DOI
  41. Von Pawel-Rammingen, 2000, GAP activity of the Yersinia YopE cytotoxin specifically targets the rho pathway, Mol. Microbiol., 36, 737, 10.1046/j.1365-2958.2000.01898.x · DOI
  42. Wittinghofer, 1997, Signaling mechanistics, Curr. Biol., 7, R682, 10.1016/S0960-9822(06)00355-1 · DOI

Cited by

See all 160

Metadata

Authors
Stebbins, C. E., & Galán, J. E.
Venue
Molecular Cell
Publisher
Elsevier BV
Published
2000-12
Volume
6
Issue
6
Pages
1449-1460
ISSN
1097-2765
DOI
10.1016/s1097-2765(00)00141-6
Cited by
160
References
3
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Modulation of Host Signaling by a Bacterial Mimic. https://app.cochat.ai/papers/10.1016/s1097-2765(00)00141-6
The paper
Stebbins, C. E., & Galán, J. E. (2000). Modulation of Host Signaling by a Bacterial Mimic. Molecular Cell. https://doi.org/10.1016/s1097-2765(00)00141-6