The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Levels of Polyadenylation Factor CstF-64 Control IgM Heavy Chain mRNA Accumulation and Other Events Associated with B Cell Differentiation
Open accessAbstract
Cleavage stimulation factor (CstF) is one of the multiple factors required for mRNA polyadenylation. The concentration of one CstF subunit (CstF-64) increases during activation of B cells, and this is sufficient to switch IgM heavy chain mRNA expression from membrane-bound form to secreted form. To extend this observation, we disrupted the endogenous CstF-64 gene in the B cell line DT40 and replaced it with a regulatable transgene. Strikingly, a 10-fold decrease in CstF-64 concentration did not markedly affect cell growth but specifically and dramatically reduced accumulation of IgM heavy chain mRNA. Further reduction caused reversible cell cycle arrest in G0/G1 phase, while depletion resulted in apoptotic cell death. Our results indicate that CstF-64 plays unexpected roles in regulating gene expression and cell growth in B cells.
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Cites
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Gossen, M., & Bujard, H. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.12.5547 · Cited by 4,943
Mechanism and regulation of mRNA polyadenylation
Colgan, D. F., & Manley, J. L. (1997) · Genes & Development · doi:10.1101/gad.11.21.2755 · Cited by 784
Self-citation
Cited by
Sandberg, R., Neilson, J. R., Sarma, A., Sharp, P. A., & Burge, C. B. (2008) · Science · doi:10.1126/science.1155390 · Cited by 1,349
Integrating mRNA Processing with Transcription
Proudfoot, N. J., Furger, A., & Dye, M. J. (2002) · Cell · doi:10.1016/s0092-8674(02)00617-7 · Cited by 1,082
Mechanisms and Consequences of Alternative Polyadenylation
Di Giammartino, D. C., Nishida, K., & Manley, J. L. (2011) · Molecular Cell · doi:10.1016/j.molcel.2011.08.017 · Cited by 737
Self-citationFast-Find: A novel computational approach to analyzing combinatorial motifs
Hamady, M., Peden, E., Knight, R., & Singh, R. (2006) · BMC Bioinformatics · doi:10.1186/1471-2105-7-1 · Cited by 543
The Poly(A)-Binding Protein Nuclear 1 Suppresses Alternative Cleavage and Polyadenylation Sites
Jenal, M., Elkon, R., Loayza-Puch, F., van Haaften, G., Kühn, U., Menzies, F. M., Vrielink, J. A. F. O., Bos, A. J., Drost, J., Rooijers, K., et al. (2012) · Cell · doi:10.1016/j.cell.2012.03.022 · Cited by 339
Metadata
- Authors
- Takagaki, Y., & Manley, J. L.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 1998-12
- Volume
- 2
- Issue
- 6
- Pages
- 761-771
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2079932405
- Cited by
- 223
- References
- 2
- FWCI
- 2.81
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Levels of Polyadenylation Factor CstF-64 Control IgM Heavy Chain mRNA Accumulation and Other Events Associated with B Cell Differentiation. https://app.cochat.ai/papers/10.1016/s1097-2765(00)80291-9
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- Takagaki, Y., & Manley, J. L. (1998). Levels of Polyadenylation Factor CstF-64 Control IgM Heavy Chain mRNA Accumulation and Other Events Associated with B Cell Differentiation. Molecular Cell. https://doi.org/10.1016/s1097-2765(00)80291-9
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