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The Mechanism of Intrinsic Transcription Termination
Open accessAbstract
In bacteria, an intrinsic transcription termination signal appears in RNA as a hairpin followed by approximately eight uridines (U stretch) at the 3' terminus. This signal leads to rapid dissociation of the ternary elongation complex (TEC) into RNA, DNA, and an RNA polymerase. We demonstrate that the hairpin inactivates and then destabilizes TEC by weakening interactions in the RNA-DNA hybrid-binding site and the RNA-binding site that hold TEC together. Formation of the hairpin is restricted to the moment when TEC reaches the point of termination and depends upon melting of four to five hybrid base pairs that follow the hairpin's stem. The U stretch-induced pausing at the point of termination is crucial, providing additional time for hairpin formation. These results explain the mechanism of termination and aid in understanding of how cellular factors modulate this process.
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Cited by
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Proshkin, S., Rahmouni, A. R., Mironov, A., & Nudler, E. (2010) · Science · doi:10.1126/science.1184939 · Cited by 561
Self-citationAdenine riboswitches and gene activation by disruption of a transcription terminator
Mandal, M., & Breaker, R. R. (2004) · Nature Structural & Molecular Biology · doi:10.1038/nsmb710 · Cited by 514
Prospects for riboswitch discovery and analysis
Breaker, R. R. (2011) · Molecular Cell · doi:10.1016/j.molcel.2011.08.024 · Cited by 464
Structure and Mechanism of a Metal-Sensing Regulatory RNA
Dann, C. E., Wakeman, C. A., Sieling, C. L., Baker, S. C., Irnov, I., & Winkler, W. C. (2007) · Cell · doi:10.1016/j.cell.2007.06.051 · Cited by 386
A pause sequence enriched at translation start sites drives transcription dynamics in vivo
Larson, M. H., Mooney, R. A., Peters, J. M., Windgassen, T., Nayak, D., Gross, C. A., Block, S. M., Greenleaf, W. J., Landick, R., & Weissman, J. S. (2014) · Science · doi:10.1126/science.1251871 · Cited by 317
Metadata
- Authors
- Gusarov, I., & Nudler, E.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 1999-04
- Volume
- 3
- Issue
- 4
- Pages
- 495-504
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W1987568156
- Cited by
- 379
- References
- 0
- FWCI
- 5.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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