The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Generation and characterization of chimeric recombinant AAV vectors
Open accessAbstract
Although most animal experiments with recombinant adeno-associated virus (AAV) vectors have been based on AAV serotype 2, recent studies showed that AAV vectors based on AAV serotype 1 performed more efficiently in muscle and other tissues. On the other hand, AAV2-based vectors can be readily purified by heparin column. To combine the advantages of both types of vectors, we developed a strategy to generate chimeric vectors by using a mixture of AAV helper plasmids encoding both serotypes in the transfection process. Because the AAV packaging machinery cannot distinguish between closely related AAV1 and AAV2 capsid proteins, each packaged virion contains capsid proteins from both serotypes. As expected, the resulting chimeric vectors could be purified by heparin column. Neutralizing antibody assays showed that the chimeric vectors can be inhibited by either AAV1 or AAV2 antiserum. In vivo, the chimeric vectors direct levels of expression similar to those of AAV1 in muscle or AAV2 in liver; that is, they combine the best transduction characteristics of both parent vectors. In summary, this study provides a straightforward method for combining various properties of different AAV serotypes into one vector. Potential limitations of the chimeric vectors are also discussed.
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Cites
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“A variety of transgenes using AAV vectors have been tested in brain, liver, muscle, and other tissues and positive results have been published [10-14].”
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Cited by
Grimm, D., Lee, J. S., Wang, L., Desai, T., Akache, B., Storm, T. A., & Kay, M. A. (2008) · Journal of Virology · doi:10.1128/jvi.00254-08 · Cited by 696
Adeno-associated virus serotype 8 efficiently delivers genes to muscle and heart
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Optical activation of lateral amygdala pyramidal cells instructs associative fear learning
Johansen, J. P., Hamanaka, H., Monfils, M. H., Behnia, R., Deisseroth, K., Blair, H. T., & LeDoux, J. E. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1002418107 · Cited by 308
Intracellular trafficking of adeno-associated viral vectors
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AAV Capsid Structure and Cell Interactions
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Metadata
- Authors
- Hauck, B., Chen, L., & Xiao, W.
- Venue
- Molecular Therapy
- Publisher
- Elsevier BV
- Published
- 2003-03
- Volume
- 7
- Issue
- 3
- Pages
- 419-425
- ISSN
- 1525-0016 (ISSN-L)
- OpenAlex
- W2047848268
- Cited by
- 160
- References
- 7
- FWCI
- 6.20
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
