Hidden Plasmodium falciparum parasites in human infections: different genotype distribution in the peripheral circulation and in the placenta
Abstract
Sequestration of the mature Plasmodium falciparum forms complicates detection, quantification and molecular analysis of human infections. Whether the circulating parasites represent all or only a subset of co-infecting genotypes is unclear. We have investigated this issue and compared placenta and peripheral blood msp1 and msp2 genotypes in 58 women delivering with an ICT-positive placenta in Guediawaye, Senegal. Most placenta (91%) and blood samples (98%) were multiply infected. Multiplicity of infection was positively correlated in both tissues. However, the placental and circulating genotype profiles differed markedly. Only 10% of matched peripheral blood/placenta samples had identical genotypes, whereas 74% had only partially concordant genotypes, with some alleles detected in both tissues, together with additional allele(s) detected in one tissue only. Eight women (14%) had totally discordant placental and peripheral blood genotypes. Thus, in the vast majority of cases, some sequestered genotypes remain hidden, undetected in the peripheral circulation, indicating that analysis of peripheral parasites generates a partial picture of a P. falciparum infection.
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More parasites in the field carry resistant genotypes.
“even though more and more parasites now in the field carry resistant genotypes (Schleiermacher et al. , 2002).”
From Towards a vaccine against pregnancy-associated malaria (2008) · cites this paper as Schleiermacher et al., 2002
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WALKER-ABBEY, A., DJOKAM, R. R. T., ENO, A., LEKE, R. F. G., TITANJI, V. P. K., FOGAKO, J., SAMA, G., THUITA, L. H., BEARDSLEE, E., SNOUNOU, G., et al. (2005) · The American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2005.72.229 · Cited by 132 · see citing works
Mayengue, P. I., Rieth, H., Khattab, A., Issifou, S., Kremsner, P. G., Klinkert, M., & Ntoumi, F. (2004) · Tropical Medicine & International Health · doi:10.1111/j.1365-3156.2004.01294.x · Cited by 50 · see citing works
Arango, E., Maestre, A., & Carmona-Fonseca, J. (2010) · Revista Brasileira de Epidemiologia · doi:10.1590/s1415-790x2010000300002 · Cited by 33 · see citing works
Arango, E. M., Samuel, R., Agudelo, O. M., Carmona-Fonseca, J., Maestre, A., & Yanow, S. K. (2012) · Malaria Journal · doi:10.1186/1475-2875-11-392 · Cited by 18 · see citing works
Towards a vaccine against pregnancy-associated malaria
Tuikue Ndam, N., & Deloron, P. (2008) · Parasite · doi:10.1051/parasite/2008153515 · Cited by 14 · see citing works
- Authors
- Schleiermacher, D., Le Hesran, J.-Y., Ndiaye, J.-L., Perraut, R., Gaye, A., & Mercereau-Puijalon, O.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Institut de Recherche pour le Développement (SN)
- Institut Pasteur (FR)
- Venue
- Infection, Genetics and Evolution
- Publisher
- Elsevier BV
- Published
- 2002-12
- Volume
- 2
- Issue
- 2
- Pages
- 97-105
- ISSN
- 1567-1348 (ISSN-L)
- OpenAlex
- W2146885083
- Cited by
- 22
- References
- 0
- Citations by year
- 2024: 1 · 2018: 2 · 2017: 2 · 2016: 1 · 2012: 3
- FWCI
- 2.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Infection Genetics and Evolution (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Health Sciences › Medicine › Genetics › Hemoglobinopathies and Related Disorders
- Life Sciences › Immunology and Microbiology › Parasitology › Bird parasitology and diseases
- Keywords
- Plasmodium falciparum, placental malaria, sequestration, merozoite surface protein 1, msp2, genotype distribution, multiplicity of infection, co-infection, placenta, peripheral blood, placental sequestration
- MeSH terms
- Animals, Female, Gene Frequency, Humans, Placenta, Plasmodium falciparum, Malaria, Falciparum
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