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Cited by 140Bone Metabolism and DiseasesCell Adhesion Molecules ResearchBone and Dental Protein Studies

The DOI matches its Crossref record; 54 of the 55 references it cites were found in a registry or an index.

β1 integrins and osteoclast function: Involvement in collagen recognition and bone resorption

Helfrich, M.H., Nesbitt, S.A., Lakkakorpi, P.T., Barnes, M.J., Bodary, S.C., Shankar, G., Mason, W.T., Mendrick, D.L., Väänänen, H.K., Horton, M.A.·1996·Bone
DOI 10.1016/s8756-3282(96)00223-2
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Abstract

The extracellular matrix of bone is composed mainly of type I collagen. In this report we studied the role and collagen-binding properties of osteoclast integrins (alpha v, alpha 2, beta 1, and beta 3). Cell adhesion assays with rat osteoclasts and affinity chromatography/SDS-PAGE analysis with purified human osteoclast membranes demonstrated adhesion of osteoclasts to native type I collagen in a divalent cation and Arg-Gly-Asp (RGD)-dependent way via alpha 2 beta 1 integrin, whereas osteoclast adhesion to denatured collagen predominantly involved alpha v beta 3. In receptor-binding assays, the involvement of human recombinant alpha v beta 3 in adhesion to denatured collagen was confirmed. Additionally, osteoclasts adhered to type I collagen fibers and to monomeric types II-V collagen with characteristics similar to those on native monomeric type I collagen. Osteoclastic bone resorption in vitro was inhibited (> 40%) in the presence of alpha 2 and beta 1 antibodies. Using scanning laser confocal microscopy, alpha v beta 3, alpha 2, and beta 1 integrin were detected within podosomes in nonresorbing osteoclasts and in the ruffled border area and basolateral membrane in resorbing osteoclasts, but not in the sealing zone of resorbing osteoclasts. These results demonstrate that alpha 2 beta 1, in addition to alpha v beta 3, has an important role in osteoclast function and acts as a receptor for native, but not denatured, collagen.

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Crossref · 10.1016/s8756-3282(96)00223-2

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Research works printed but not linked: 2

  1. Masarachia, 1995, Colocalization of the vitronectin receptor αvβ3 and echistatin in osteoclasts during bone resorption, J Bone Miner Res, 10, S164
  2. Neff, 1995, Ultrastructural demonstration of the av subunit of the vitronectin receptor in the sealing zone of resorbing osteoclasts, J Bone Miner Res, 10, S329

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Metadata

Authors
Helfrich, M. H., Nesbitt, S. A., Lakkakorpi, P. T., Barnes, M. J., Bodary, S. C., Shankar, G., Mason, W. T., Mendrick, D. L., Väänänen, H. K., & Horton, M. A.
Affiliations
  • University of Aberdeen (GB)
  • University College London (GB)
  • University of Oulu (FI)
  • University of Cambridge (GB)
  • Genentech (US)
  • Babraham Institute (GB)
  • Brigham and Women's Hospital (US)
Corresponding author
Helfrich, M.H.
ORCID
Venue
Bone
Publisher
Elsevier BV
Published
1996-10
Volume
19
Issue
4
Pages
317-328
ISSN
  • 1873-2763 (ISSN-L)
  • 8756-3282
DOI
10.1016/s8756-3282(96)00223-2
OpenAlex
W2107556429
Cited by
139
References
55
Citations by year
2026: 2 · 2024: 6 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 5 · 2019: 4 · 2018: 6 · 2017: 3 · 2016: 1 · 2015: 5 · 2014: 5 · 2013: 5 · 2012: 7
FWCI
2.40
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Bone (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Bone and Dental Protein Studies
Keywords
beta1 integrin, osteoclasts, bone resorption, type I collagen, alpha2beta1 integrin, integrin αvβ3, RGD motif, cell adhesion, podosomes, ruffled border, sealing zone, denatured collagen, integrin antibodies
MeSH terms
Amino Acid Sequence, Animals, Antibodies, Monoclonal, Binding, Competitive, Bone Resorption, Cations, Divalent, Cell Adhesion, Chromatography, Affinity, Collagen, Electrophoresis, Polyacrylamide Gel, Humans, Immunohistochemistry, Membrane Proteins, Oligopeptides, Osteoclasts, Peptide Fragments, Receptors, Immunologic, Integrins, Rats

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