Cited by 53Probiotics and Fermented FoodsEnzyme Production and CharacterizationBacterial Genetics and Biotechnology

Comparison of cell surface proteinase activities within the Lactobacillus genus

GILBERT, CHRISTOPHE, BLANC, BRIGITTE, FROT-COUTAZ, JACQUES, PORTALIER, RAYMOND, ATLAN, DANIÈLE·1997·Journal of Dairy Research

Abstract

Whole cells of Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397 (Lb. bulgaricus CNRZ 397) are able to hydrolyse α- and β-caseins. We have isolated a mutant of Lb. bulgaricus altered for growth in milk and unable to hydrolyse α- or β-casein. Normal growth was restored by adding amino acids or tryptone to milk. No significant difference between the peptidase activities of parent and mutant strains was observed. The cell surface caseinolytic activities of three lactobacilli species and Lactococcus lactis subsp. lactis (Lc. lactis) were compared. As expected, the characteristics of the cell surface proteinase activity of Lb. casei were similar to those of Lc. lactis. We showed that the cleavage specificities of the cell surface proteinase activities from lactobacilli were species-dependent and at least three types of activity were distinguished. The regulation of the biosynthesis of cell surface proteinase activities was medium-dependent and different within the Lactobacillus genus and even within the Lb. delbrueckii species. In contrast to Lb. bulgaricus, the cell surface proteinase activity of Lb. lactis was totally inhibited in a medium rich in peptides or amino acids. In contrast, the cell surface of Lb. helveticus probably displayed two proteinases with different cleavage specificities and with a biosynthesis regulation sensitive to different medium components.

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Crossref · 10.1017/s0022029997002355

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Authors
GILBERT, C., BLANC, B., FROT-COUTAZ, J., PORTALIER, R., & ATLAN, D.
Affiliations
Lyon 1 Université (FR)
Venue
Journal of Dairy Research
Published
1997-11
Volume
64
Issue
4
Pages
561-571
ISSN
  • 0022-0299 (ISSN-L)
  • 1469-7629
OpenAlex
W2066157430
Cited by
53
References
0
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2020: 1 · 2017: 2 · 2016: 1 · 2014: 2 · 2013: 5 · 2012: 4
FWCI
4.75
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Dairy Research (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Lactobacillus bulgaricus, Lactobacillus helveticus, casein hydrolysis, alpha-casein, β-casein, dairy starter cultures

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