Comparison of cell surface proteinase activities within the Lactobacillus genus
Abstract
Whole cells of Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397 (Lb. bulgaricus CNRZ 397) are able to hydrolyse α- and β-caseins. We have isolated a mutant of Lb. bulgaricus altered for growth in milk and unable to hydrolyse α- or β-casein. Normal growth was restored by adding amino acids or tryptone to milk. No significant difference between the peptidase activities of parent and mutant strains was observed. The cell surface caseinolytic activities of three lactobacilli species and Lactococcus lactis subsp. lactis (Lc. lactis) were compared. As expected, the characteristics of the cell surface proteinase activity of Lb. casei were similar to those of Lc. lactis. We showed that the cleavage specificities of the cell surface proteinase activities from lactobacilli were species-dependent and at least three types of activity were distinguished. The regulation of the biosynthesis of cell surface proteinase activities was medium-dependent and different within the Lactobacillus genus and even within the Lb. delbrueckii species. In contrast to Lb. bulgaricus, the cell surface proteinase activity of Lb. lactis was totally inhibited in a medium rich in peptides or amino acids. In contrast, the cell surface of Lb. helveticus probably displayed two proteinases with different cleavage specificities and with a biosynthesis regulation sensitive to different medium components.
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Most L. bulgaricus strains are characterized by a high CSP activity resulting from adaptation of this species to fast growth and rapid fermentation of milk.
“Most L. bulgaricus strains are characterized by a high CSP activity resulting from the adaptation of this species to fast growth and rapid fermentation of milk (14).”
Most LAB possess only one CEP but L. helveticus has been reported to have at least two: PrtH and PrtH2.
“Most of the LAB possess only one CEP but L. helveticus has been reported to have at least two: PrtH and PrtH2 (Gilbert et al., 1997).”
From Lactobacillus helveticus: the proteolytic system (2013) · cites this paper as Gilbert et al., 1997
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Griffiths, M. W., & Tellez, A. M. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00030 · Cited by 241 · see citing works
“PrtH1 and PrtH2 present in L. helveticus differ from the CEPs of other LAB because they lack the anchor domain (AN), which is located at the C-terminus of the CEP sequence.”
BackgroundMulti-domain, cell-envelope proteinases of lactic acid bacteria.
Siezen, R. J. (1999) · Antonie van Leeuwenhoek · doi:10.1023/a:1002036906922 · Cited by 144 · see citing works
Antihypertensive activity of casein-enriched milk fermented by Lactobacillus helveticus
Leclerc, P.-L., Gauthier, S. F., Bachelard, H., Santuré, M., & Roy, D. (2002) · International Dairy Journal · doi:10.1016/s0958-6946(02)00125-5 · Cited by 143 · see citing works
Robert, M.-C., Razaname, A., Mutter, M., & Juillerat, M. A. (2004) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf049510t · Cited by 142 · see citing works
Broadbent, J. R., Cai, H., Larsen, R. L., Hughes, J. E., Welker, D. L., De Carvalho, V. G., Tompkins, T. A., Ardö, Y., Vogensen, F., De Lorentiis, A., et al. (2011) · Journal of Dairy Science · doi:10.3168/jds.2010-4068 · Cited by 124 · see citing works
Original features of cell-envelope proteinases of Lactobacillus helveticus. A review
Sadat-Mekmene, L., Genay, M., Atlan, D., Lortal, S., & Gagnaire, V. (2011) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2011.01.039 · Cited by 119 · see citing works
Self-citationCourtin, P., Monnet, V., & Rul, F. (2002) · Microbiology · doi:10.1099/00221287-148-11-3413 · Cited by 111 · see citing works
LeBlanc, J., Fliss, I., & Matar, C. (2004) · Clinical and Vaccine Immunology · doi:10.1128/cdli.11.6.1171-1181.2004 · Cited by 106 · see citing works
Pederson, J. A., Mileski, G. J., Weimer, B. C., & Steele, J. L. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.15.4592-4597.1999 · Cited by 91 · see citing works
Lactobacillus delbrueckii ssp. lactis and ssp. bulgaricus: a chronicle of evolution in action
Kafsi, H. E., Binesse, J., Loux, V., Buratti, J., Boudebbouze, S., Dervyn, R., Kennedy, S., Galleron, N., Quinquis, B., Batto, J.-M., et al. (2014) · BMC Genomics · doi:10.1186/1471-2164-15-407 · Cited by 90 · see citing works
Germond, J.-E., Lapierre, L., Delley, M., Mollet, B., Felis, G. E., & Dellaglio, F. (2003) · Molecular Biology and Evolution · doi:10.1093/molbev/msg012 · Cited by 73 · see citing works
Sadat-Mekmene, L., Jardin, J., Corre, C., Mollé, D., Richoux, R., Delage, M.-M., Lortal, S., & Gagnaire, V. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.01466-10 · Cited by 72 · see citing works
Genomic Evolution of Domesticated Microorganisms
Douglas, G. L., & Klaenhammer, T. R. (2010) · Annual Review of Food Science and Technology · doi:10.1146/annurev.food.102308.124134 · Cited by 70 · see citing works
Genay, M., Sadat, L., Gagnaire, V., & Lortal, S. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.02395-08 · Cited by 66 · see citing works
Morel, F., Frot-Coutaz, J. P., Aubel, D., Portalier, R. C., & Atlan, D. (1999) · Microbiology · doi:10.1099/13500872-145-2-437 · Cited by 66 · see citing works
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Williams, A. G., Noble, J., Tammam, J., Lloyd, D., & Banks, J. M. (2002) · International Dairy Journal · doi:10.1016/s0958-6946(02)00063-8 · Cited by 47 · see citing works
Arioli, S., Monnet, C., Guglielmetti, S., Parini, C., Noni, I. D., Hogenboom, J. A., Halami, P. M., & Mora, D. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.00533-07 · Cited by 45 · see citing works
Oberg, C. J., Broadbent, J. R., Strickland, M., & McMahon, D. J. (2002) · Letters in Applied Microbiology · doi:10.1046/j.1472-765x.2002.01123.x · Cited by 40 · see citing works
Lamarque, M. H., Charbonnel, P., Aubel, D., Piard, J.-C., Atlan, D., & Juillard, V. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.19.6492-6500.2004 · Cited by 39 · see citing works
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Germond, J.-E., Delley, M., Gilbert, C., & Atlan, D. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.6.3377-3384.2003 · Cited by 36 · see citing works
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Ahmadova, A., El‐Ghaish, S., Choiset, Y., Rabesona, H., Drouet, M., Chobert, J., Kuliev, A. A., & Haertlé, T. (2012) · Journal of Food Biochemistry · doi:10.1111/j.1745-4514.2012.00664.x · Cited by 31 · see citing works
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Hébert, E. M., Raya, R. R., & de Giori, G. S. (2002) · Current Microbiology · doi:10.1007/s00284-002-3780-z · Cited by 27 · see citing works
Agyei, D., Potumarthi, R., & Danquah, M. K. (2012) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-012-9839-9 · Cited by 24 · see citing works
Oliszewski, R., Wolf, I. V., Bergamini, C. V., Candioti, M. C., & Perotti, M. C. (2013) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.6092 · Cited by 22 · see citing works
Tamime, A. Y., & Robinson, R. K. (2007) · Elsevier eBooks · doi:10.1533/9781845692612.535 · Cited by 21 · see citing works
Bergamini, C. V., Hynes, E. R., Candioti, M. C., & Zalazar, C. A. (2009) · Journal of Dairy Science · doi:10.3168/jds.2008-1794 · Cited by 19 · see citing works
Skrzypczak, K., Gustaw, W., Jabłońska-Ryś, E. D., Michalak‐Majewska, M., Sławińska, A., Radzki, W. P., Gustaw, K. M., & Waśko, A. D. (2017) · Acta Scientiarum Polonorum Technologia Alimentaria · doi:10.17306/j.afs.0479 · Cited by 19 · see citing works
Sfaxi, I. H., El‐Ghaish, S., Ahmadova, A., Rabesona, H., Haertlé, T., & Chobert, J. (2012) · European Food Research and Technology · doi:10.1007/s00217-012-1772-1 · Cited by 16 · see citing works
Liu, Q., Wang, H., Zhu, W., Peng, S., Zou, H., Zhang, P., Li, Z., Zhang, Z., Fu, L., & Qian, Z. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.133958 · Cited by 12 · see citing works
Ritt, J., Remize, F., Grandvalet, C., Guzzo, J., Atlan, D., & Alexandre, H. (2009) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2008.04032.x · Cited by 12 · see citing works
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Siezen, R. J. (1999) · Springer eBooks · doi:10.1007/978-94-017-2027-4_6 · Cited by 10 · see citing works
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Marathe, M. Y., & Ghosh, J. (2009) · Journal of genetics and molecular biology · doi:10.5897/ijgmb.9000059 · Cited by 9 · see citing works
Lim, S.-D., Kim, K.-S., & Do, J. (2008) · Korean Journal for Food Science of Animal Resources · doi:10.5851/kosfa.2008.28.5.587 · Cited by 9 · see citing works
Agyei, D., Potumarthi, R., & Danquah, M. K. (2013) · Wiley eBooks · doi:10.1002/9781118375082.ch11 · Cited by 9 · see citing works
Lamarque, M. H., Morel, F., Bissardon, I., Galinier, A., Portalier, R. C., & Atlan, D. (2001) · Le Lait · doi:10.1051/lait:2001123 · Cited by 7 · see citing works
Self-citationAgyei, D., Potumarthi, R., & Danquah, M. K. (2013) · Wiley eBooks · doi:10.1002/9781118375082.ch10 · Cited by 7 · see citing works
Yamamoto, E. M., Tooyama, E., & Honme, Y. (2024) · Journal of Dairy Science · doi:10.3168/jds.2023-24091 · Cited by 6 · see citing works
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Samartsev, A. A., Astapovich, N. I., & Novik, G. I. (2000) · Microbiology · doi:10.1023/a:1026697906562 · Cited by 5 · see citing works
Gu, L., Tadesse, B. T., Solem, C., & Zhao, S. (2025) · Food Bioscience · doi:10.1016/j.fbio.2025.107046 · Cited by 4 · see citing works
Rubak, Y. T., Nuraida, L., Iswantini, D., & Prangdimur, E. (2020) · Pakistan Journal of Nutrition · doi:10.3923/pjn.2020.295.302 · Cited by 4 · see citing works
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Lortal, S. (2004) · CRC Press eBooks · doi:10.1201/9780203913550.ch16 · Cited by 4 · see citing works
Chen, L., Lei, N., Du, G., Chen, H., & Shu, G. (2025) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2025.110723 · Cited by 1 · see citing works
Hasanov, R., Jebbar, M., Caroff, N., & Kuliev, A. A. (2012)
Li, H., Zheng, Q., & Fan, X. (2026) · Applied Food Research · doi:10.1016/j.afres.2026.101736
- Authors
- GILBERT, C., BLANC, B., FROT-COUTAZ, J., PORTALIER, R., & ATLAN, D.
- Affiliations
- Lyon 1 Université (FR)
- ORCID
- GILBERT, CHRISTOPHE
- Venue
- Journal of Dairy Research
- Publisher
- Cambridge University Press (CUP)
- Published
- 1997-11
- Volume
- 64
- Issue
- 4
- Pages
- 561-571
- ISSN
- 0022-0299 (ISSN-L)
- 1469-7629
- OpenAlex
- W2066157430
- Cited by
- 53
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2020: 1 · 2017: 2 · 2016: 1 · 2014: 2 · 2013: 5 · 2012: 4
- FWCI
- 4.75
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Dairy Research (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Lactobacillus bulgaricus, Lactobacillus helveticus, casein hydrolysis, alpha-casein, β-casein, dairy starter cultures
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