The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Liquid Chromatography/Mass Spectrometry of Phospholipids using Electrospray Ionization
Abstract
An improved technique for phospholipid molecular species analysis was developed using high-performance liquid chromatography/mass spectrometry with the electrospray interface. Using the 0.5% ammonium hydroxide in a water-methanol-hexane mixture and a C-18 column, complex mixtures of phospholipid molecular species were separated and detected mainly as protonated or natriated molecular species. The response was linear over 2 orders of magnitude, allowing quantification of each molecular species. In comparison to the existing LC/MS techniques, marked improvement in sensitivity was observed. The present quantification limit is approximately 0.5 pmol before split (5 fmol after 1/100 split). The relative responses were more dependent on the head group identity rather than fatty acyl composition within a phospholipid class. In general, phosphatidylcholine (PC) species are most sensitively detected followed by phosphatidylethanolamine (PE) species. The sensitivity of phosphatidylserine (PS) in the positive ion mode is approximately 20 times less in comparison to PC under our condition.
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Citations
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Electrospray Ionization for Mass Spectrometry of Large Biomolecules
Fenn, J. B., Mann, M., Meng, C. K., Wong, S. F., & Whitehouse, C. M. (1989) · Science · doi:10.1126/science.2675315 · Cited by 7,634
Cited by
Schneiter, R., Brügger, B., Sandhoff, R., Zellnig, G., Leber, A., Lampl, M., Athenstaedt, K., Hrastnik, C., Eder, S., Daum, G., et al. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.146.4.741 · Cited by 493
Koivusalo, M., Haimi, P., Heikinheimo, L., Kostiainen, R., & Somerharju, P. (2001) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)31176-7 · Cited by 417
Han, X., & Gross, R. W. (1995) · Journal of the American Society for Mass Spectrometry · doi:10.1016/1044-0305(95)00568-4 · Cited by 289
Han, X., Yang, K., & Gross, R. W. (2008) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.3595 · Cited by 190
Metadata
- Authors
- Kim, H.-Y., Wang, T.-C. L., & Ma, Y.-C.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1994-11-15
- Volume
- 66
- Issue
- 22
- Pages
- 3977-3982
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2094561952
- Cited by
- 259
- References
- 2
- FWCI
- 2.91
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kim, H.-Y., Wang, T.-C. L., & Ma, Y.-C. (1994). Liquid Chromatography/Mass Spectrometry of Phospholipids using Electrospray Ionization. Analytical Chemistry. https://doi.org/10.1021/ac00094a020
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
