Microfluidics‐based electrospray ionization enhances the intrasource separation of lipid classes and extends identification of individual molecular species through multi‐dimensional mass spectrometry: development of an automated high‐throughput platform for shotgun lipidomics

The emerging field of lipidomics

Wenk, M. R. (2005) · Nature Reviews Drug Discovery · doi:10.1038/nrd1776 · Cited by 1,325 · see citing works

Shotgun lipidomics: Electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples

Han, X., & Gross, R. W. (2004) · Mass Spectrometry Reviews · doi:10.1002/mas.20023 · Cited by 1,111 · see citing works

Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry: a bridge to lipidomics

Han, X., & Gross, R. W. (2003) · Journal of Lipid Research · doi:10.1194/jlr.r300004-jlr200 · Cited by 914 · see citing works

Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry

Brügger, B., Erben, G., Sandhoff, R., Wieland, F., & Lehmann, W. D. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.6.2339 · Cited by 882 · see citing works

Electrospray ionization mass spectroscopic analysis of human erythrocyte plasma membrane phospholipids.

Han, X., & Gross, R. W. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.22.10635 · Cited by 436 · see citing works

Quantitative determination of phospholipid compositions by ESI-MS: effects of acyl chain length, unsaturation, and lipid concentration on instrument response

Koivusalo, M., Haimi, P., Heikinheimo, L., Kostiainen, R. K., & Somerharju, P. J. (2001) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)31176-7 · Cited by 417 · see citing works

Automated Identification and Quantification of Glycerophospholipid Molecular Species by Multiple Precursor Ion Scanning

Ejsing, C. S., Duchoslav, E., Sampaio, J. L., Simons, K., Bonner, R. F., Thiele, C., Ekroos, K., & Shevchenko, A. (2006) · Analytical Chemistry · doi:10.1021/ac060545x · Cited by 416 · see citing works

Quantitative Analysis and Molecular Species Fingerprinting of Triacylglyceride Molecular Species Directly from Lipid Extracts of Biological Samples by Electrospray Ionization Tandem Mass Spectrometry

Han, X., & Gross, R. W. (2001) · Analytical Biochemistry · doi:10.1006/abio.2001.5178 · Cited by 358 · see citing works

Lipid Profiling by Multiple Precursor and Neutral Loss Scanning Driven by the Data-Dependent Acquisition

Schwudke, D., Oegema, J., Burton, L., Entchev, E. V., Hannich, J. T., Ejsing, C. S., Kurzchalia, T. V., & Shevchenko, A. (2005) · Analytical Chemistry · doi:10.1021/ac051605m · Cited by 292 · see citing works

Structural determination of picomole amounts of phospholipids via electrospray ionization tandem mass spectrometry

Han, X., & Gross, R. W. (1995) · Journal of the American Society for Mass Spectrometry · doi:10.1016/1044-0305(95)00568-4 · Cited by 290 · see citing works

Shotgun lipidomics: multidimensional MS analysis of cellular lipidomes

Han, X., & Gross, R. W. (2005) · Expert Review of Proteomics · doi:10.1586/14789450.2.2.253 · Cited by 278 · see citing works

Alterations in Myocardial Cardiolipin Content and Composition Occur at the Very Earliest Stages of Diabetes: A Shotgun Lipidomics Study

Han, X., Yang, J., Yang, K., Zhao, Z., Abendschein, D. R., & Gross, R. W. (2007) · Biochemistry · doi:10.1021/bi7004015 · Cited by 273 · see citing works

Liquid Chromatography/Mass Spectrometry of Phospholipids using Electrospray Ionization

Kim, H. Y., Wang, T.-C. L., & Ma, Y.-C. (1994) · Analytical Chemistry · doi:10.1021/ac00094a020 · Cited by 260 · see citing works

Toward fingerprinting cellular lipidomes directly from biological samples by two-dimensional electrospray ionization mass spectrometry

Han, X., Yang, J., Cheng, H., Ye, H., & Gross, R. W. (2004) · Analytical Biochemistry · doi:10.1016/j.ab.2004.04.004 · Cited by 219 · see citing works

Automated Quantitative Analysis of Complex Lipidomes by Liquid Chromatography/Mass Spectrometry

Hermansson, M., Uphoff, A., Käkelä, R., & Somerharju, P. J. (2005) · Analytical Chemistry · doi:10.1021/ac048489s · Cited by 217 · see citing works

Top-Down Lipidomic Screens by Multivariate Analysis of High-Resolution Survey Mass Spectra

Schwudke, D., Hannich, J. T., Surendranath, V., Grimard, V., Moehring, T., Burton, L., Kurzchalia, T. V., & Shevchenko, A. (2007) · Analytical Chemistry · doi:10.1021/ac062455y · Cited by 186 · see citing works

Shotgun lipidomics of cardiolipin molecular species in lipid extracts of biological samples

Han, X., Yang, K., Yang, J., Cheng, H., & Gross, R. W. (2006) · Journal of Lipid Research · doi:10.1194/jlr.d500044-jlr200 · Cited by 184 · see citing works

Lipidomics is emerging

Lagarde, M., Géloën, A., Record, M., Vance, D. E., & Spener, F. (2003) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2003.11.002 · Cited by 180 · see citing works

Shotgun lipidomics of phosphoethanolamine-containing lipids in biological samples after one-step in situ derivatization

Han, X., Yang, K., Cheng, H., Fikes, K. N., & Gross, R. W. (2005) · Journal of Lipid Research · doi:10.1194/jlr.d500007-jlr200 · Cited by 164 · see citing works

MALDI-TOF MS in lipidomics

Schiller, J. (2006) · Frontiers in bioscience · doi:10.2741/2255 · Cited by 162 · see citing works

Plant lipidomics: Discerning biological function by profiling plant complex lipids using mass spectrometry

Welti, R. (2006) · Frontiers in bioscience · doi:10.2741/2250 · Cited by 161 · see citing works

Molecular species composition of rat liver phospholipids by ESI-MS/MS: the effect of chromatography

DeLong, C. J., Baker, P. R. S., Samuel, M. P., Cui, Z., & Thomas, M. J. (2001) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)31524-8 · Cited by 148 · see citing works

Alterations in Individual Molecular Species of Human Platelet Phospholipids during Thrombin Stimulation: Electrospray Ionization Mass Spectrometry-Facilitated Identification of the Boundary Conditions for the Magnitude and Selectivity of Thrombin-Induced Platelet Phospholipid Hydrolysis

Han, X., Gubitosi‐Klug, R., Collins, B. J., & Gross, R. W. (1996) · Biochemistry · doi:10.1021/bi952927v · Cited by 135 · see citing works

Factors influencing the electrospray intrasource separation and selective ionization of glycerophospholipids

Han, X., Yang, K., Yang, J., Fikes, K. N., Cheng, H., & Gross, R. W. (2006) · Journal of the American Society for Mass Spectrometry · doi:10.1016/j.jasms.2005.11.003 · Cited by 127 · see citing works

Collision‐induced dissociation pathways of yeast sphingolipids and their molecular profiling in total lipid extracts: a study by quadrupole TOF and linear ion trap–orbitrap mass spectrometry

Ejsing, C. S., Moehring, T., Bahr, U., Duchoslav, E., Karas, M., Simons, K., & Shevchenko, A. (2006) · Journal of Mass Spectrometry · doi:10.1002/jms.997 · Cited by 125 · see citing works

Characterization and direct quantitation of cerebroside molecular species from lipid extracts by shotgun lipidomics

Han, X., & Cheng, H. (2004) · Journal of Lipid Research · doi:10.1194/jlr.d400022-jlr200 · Cited by 125 · see citing works

Alterations in lipid homeostasis of mouse dorsal root ganglia induced by apolipoprotein E deficiency: a shotgun lipidomics study

Cheng, H., Jiang, X., & Han, X. (2007) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2006.04342.x · Cited by 113 · see citing works

Neurolipidomics: challenges and developments

Han, X. (2006) · Frontiers in bioscience · doi:10.2741/2258 · Cited by 110 · see citing works

LIPID Arrays: New Tools in the Understanding of Membrane Dynamics and Lipid Signaling

Ivanova, P. T. (2004) · Molecular Interventions · doi:10.1124/mi.4.2.6 · Cited by 108 · see citing works

Alkaline methanolysis of lipid extracts extends shotgun lipidomics analyses to the low-abundance regime of cellular sphingolipids

Jiang, X., Cheng, H., Yang, K., Gross, R. W., & Han, X. (2007) · Analytical Biochemistry · doi:10.1016/j.ab.2007.08.019 · Cited by 95 · see citing works

Caloric Restriction Results in Phospholipid Depletion, Membrane Remodeling, and Triacylglycerol Accumulation in Murine Myocardium

Han, X., Cheng, H., Mancuso, D. J., & Gross, R. W. (2004) · Biochemistry · doi:10.1021/bi048307o · Cited by 88 · see citing works

Characterization and Quantification of Rat Bile Phosphatidylcholine by Electrospray–Tandem Mass Spectrometry

Lehmann, W. D., Koester, M., Erben, G., & KEPPLER, D. O. R. (1997) · Analytical Biochemistry · doi:10.1006/abio.1996.9941 · Cited by 81 · see citing works

Mediator lipidomics

Serhan, C. N. (2004) · Prostaglandins & Other Lipid Mediators · doi:10.1016/j.prostaglandins.2004.09.016 · Cited by 66 · see citing works

Targeted lipidomics: fatty acid amides and pain modulation

Walker, J. M., Krey, J. F., Chen, J. S., Vefring, E., Jahnsen, J. A., Bradshaw, H. B., & Huang, S. M. (2004) · Prostaglandins & Other Lipid Mediators · doi:10.1016/j.prostaglandins.2004.09.017 · Cited by 42 · see citing works

Myocardial Lipidomics. Developments in myocardial nuclear lipidomics

Carolyn, J. A. (2006) · Frontiers in bioscience · doi:10.2741/2269 · Cited by 23 · see citing works

Direct mass spectrometric determination of bead bound compounds in a combinatorial lead discovery application

Blom, K. F., Combs, A. P., Rockwell, A. L., Oldenburg, K. R., Zhang, J.-H., & Chen, T. (1998) · Rapid Communications in Mass Spectrometry · doi:10.1002/(sici)1097-0231(19980915)12:17 · Cited by 13 · see citing works

Lipidomics for studying metabolism

Han, X. (2016) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2016.98 · Cited by 714 · see citing works

Multi‐dimensional mass spectrometry‐based shotgun lipidomics and novel strategies for lipidomic analyses

Han, X., Yang, K., & Gross, R. W. (2011) · Mass Spectrometry Reviews · doi:10.1002/mas.20342 · Cited by 530 · see citing works

Automated Lipid Identification and Quantification by Multidimensional Mass Spectrometry-Based Shotgun Lipidomics

Yang, K., Cheng, H., Gross, R. W., & Han, X. (2009) · Analytical Chemistry · doi:10.1021/ac900241u · Cited by 416 · see citing works

Circadian Clocks and Feeding Time Regulate the Oscillations and Levels of Hepatic Triglycerides

Adamovich, Y., Rousso-Noori, L., Zwighaft, Z., Neufeld-Cohen, A., Golik, M., Kraut-Cohen, J., Wang, M., Han, X., & Asher, G. (2014) · Cell Metabolism · doi:10.1016/j.cmet.2013.12.016 · Cited by 410 · see citing works

Metabolomics in Early Alzheimer's Disease: Identification of Altered Plasma Sphingolipidome Using Shotgun Lipidomics

Han, X., Rozen, S., Boyle, S. H., Hellegers, C., Cheng, H., Burke, J. R., Welsh-Bohmer, K. A., Doraiswamy, P. M., & Kaddurah-Daouk, R. (2011) · PLoS ONE · doi:10.1371/journal.pone.0021643 · Cited by 429 · see citing works

Targeting DGAT1 Ameliorates Glioblastoma by Increasing Fat Catabolism and Oxidative Stress

Cheng, X., Geng, F., Pan, M., Wu, X., Zhong, Y., Wang, C., Tian, Z., Cheng, C., Zhang, R., Puduvalli, V. K., et al. (2020) · Cell Metabolism · doi:10.1016/j.cmet.2020.06.002 · Cited by 348 · see citing works

DGAT enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes

Harris, C. A., Haas, J. T., Streeper, R. S., Stone, S. J., Kumari, M., Yang, K., Han, X., Brownell, N. K., Gross, R. W., Zechner, R., et al. (2011) · Journal of Lipid Research · doi:10.1194/jlr.m013003 · Cited by 347 · see citing works

Selection of internal standards for accurate quantification of complex lipid species in biological extracts by electrospray ionization mass spectrometry—What, how and why?

Wang, M., Wang, C., & Han, X. (2016) · Mass Spectrometry Reviews · doi:10.1002/mas.21492 · Cited by 305 · see citing works

Novel advances in shotgun lipidomics for biology and medicine

Wang, M., Wang, C., Han, R. H., & Han, X. (2015) · Progress in Lipid Research · doi:10.1016/j.plipres.2015.12.002 · Cited by 269 · see citing works

Aberrant Schwann Cell Lipid Metabolism Linked to Mitochondrial Deficits Leads to Axon Degeneration and Neuropathy

Viader, A., Sasaki, Y., Kim, S., Strickland, A. M., Workman, C. S., Yang, K., Gross, R. W., & Milbrandt, J. (2013) · Neuron · doi:10.1016/j.neuron.2013.01.012 · Cited by 270 · see citing works

High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry

Ståhlman, M., Ejsing, C. S., Tarassov, K., Perman, J., Borén, J., & Ekroos, K. (2009) · Journal of Chromatography B · doi:10.1016/j.jchromb.2009.02.037 · Cited by 216 · see citing works

Analytical Methods in Lipidomics and Their Applications

Li, M., Li, Y., Bai, Y., & Liu, H. (2013) · Analytical Chemistry · doi:10.1021/ac403554h · Cited by 210 · see citing works

Machine learning for biochemical engineering: A review

Mowbray, M., Savage, T., Wu, C., Song, Z., Cho, B. A., Del Rio-Chanona, E. A., & Zhang, D. (2021) · Biochemical Engineering Journal · doi:10.1016/j.bej.2021.108054 · Cited by 188 · see citing works

Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry — Meets a Key Challenge in Lipidomics

Yang, K., & Han, X. (2011) · Metabolites · doi:10.3390/metabo1010021 · Cited by 181 · see citing works

Fatty Acidomics: Global Analysis of Lipid Species Containing a Carboxyl Group with a Charge-Remote Fragmentation-Assisted Approach

Wang, M., Han, R. H., & Han, X. (2013) · Analytical Chemistry · doi:10.1021/ac402078p · Cited by 162 · see citing works

Lipidomics: a mass spectrometry based systems level analysis of cellular lipids

Ivanova, P. T., Milne, S. B., Myers, D. S., & Brown, H. A. (2009) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2009.08.011 · Cited by 157 · see citing works

Multi-dimensional mass spectrometry-based shotgun lipidomics and the altered lipids at the mild cognitive impairment stage of Alzheimer's disease

Han, X. (2010) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2010.01.010 · Cited by 154 · see citing works

Flow infusion electrospray ionisation mass spectrometry for high throughput, non-targeted metabolite fingerprinting: a review

Draper, J., Lloyd, A. J., Goodacre, R., & Beckmann, M. (2013) · Metabolomics · doi:10.1007/s11306-012-0449-x · Cited by 144 · see citing works

Advances in coupling microfluidic chips to mass spectrometry

Feng, X., Liu, B., Li, J., & Liu, X. (2014) · Mass Spectrometry Reviews · doi:10.1002/mas.21417 · Cited by 130 · see citing works

The mitochondria-targeted peptide SS-31 binds lipid bilayers and modulates surface electrostatics as a key component of its mechanism of action

Mitchell, W., Ng, E. A., Tamucci, J. D., Boyd, K. J., Sathappa, M., Coscia, A., Pan, M., Han, X., Eddy, N. A., May, E. R., et al. (2020) · Journal of Biological Chemistry · doi:10.1074/jbc.ra119.012094 · Cited by 126 · see citing works

Unremodeled and Remodeled Cardiolipin Are Functionally Indistinguishable in Yeast

Baile, M. G., Sathappa, M., Lu, Y.-W., Pryce, E. N., Whited, K., McCaffery, J. M., Han, X., Alder, N. N., & Claypool, S. M. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.525733 · Cited by 120 · see citing works

SREBP-1a–stimulated lipid synthesis is required for macrophage phagocytosis downstream of TLR4-directed mTORC1

Lee, J., Phelan, P. E., Shin, M., Oh, B., Han, X., Im, S., & Osborne, T. F. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1813458115 · Cited by 117 · see citing works

<i>APOE3</i> -Jacksonville (V236E) variant reduces self-aggregation and risk of dementia

Liu, C.-C., Murray, M. E., Li, X., Zhao, N., Wang, N., Heckman, M. G., Shue, F., Martens, Y., Li, Y., Raulin, A.-C., et al. (2021) · Science Translational Medicine · doi:10.1126/scitranslmed.abc9375 · Cited by 108 · see citing works

Multidimensional Mass Spectrometry-Based Shotgun Lipidomics

Wang, M., & Han, X. (2014) · Methods in molecular biology · doi:10.1007/978-1-4939-1258-2_13 · Cited by 104 · see citing works

Phosphatidylethanolamine made in the inner mitochondrial membrane is essential for yeast cytochrome bc1 complex function

Calzada, E., Avery, E., Sam, P. N., Modak, A., Wang, C., McCaffery, J. M., Han, X., Alder, N. N., & Claypool, S. M. (2019) · Nature Communications · doi:10.1038/s41467-019-09425-1 · Cited by 104 · see citing works

The Role of Microfluidics for Organ on Chip Simulations

Aziz, A. U. R., Geng, C., Fu, M., Yu, X.-H., Qin, K.-R., & Liu, B. (2017) · Bioengineering · doi:10.3390/bioengineering4020039 · Cited by 68 · see citing works

Systematic analysis of choline-containing phospholipids using multi-dimensional mass spectrometry-based shotgun lipidomics

Yang, K., Zhao, Z., Gross, R. W., & Han, X. (2009) · Journal of Chromatography B · doi:10.1016/j.jchromb.2009.01.016 · Cited by 99 · see citing works

Genetic Ablation of Calcium-independent Phospholipase A2γ Leads to Alterations in Hippocampal Cardiolipin Content and Molecular Species Distribution, Mitochondrial Degeneration, Autophagy, and Cognitive Dysfunction

Mancuso, D. J., Kotzbauer, P. T., Wozniak, D. F., Sims, H. F., Jenkins, C. M., Guan, S., Han, X., Yang, K., Sun, G., Malik, I., et al. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.055194 · Cited by 96 · see citing works

Direct Infusion Mass Spectrometry for Metabolomic Phenotyping of Diseases

González‐Domínguez, R., Sayago, A., & Fernández‐Recamales, Á. (2016) · Bioanalysis · doi:10.4155/bio-2016-0202 · Cited by 98 · see citing works

Acyl-CoA thioesterase-2 facilitates mitochondrial fatty acid oxidation in the liver

Moffat, C., Bhatia, L., Nguyen, T. T., Lynch, P., Wang, M., Wang, D., Ilkayeva, O., Han, X., Hirschey, M. D., Claypool, S. M., et al. (2014) · Journal of Lipid Research · doi:10.1194/jlr.m046961 · Cited by 88 · see citing works

Genetic Ablation of Calcium-independent Phospholipase A2γ Prevents Obesity and Insulin Resistance during High Fat Feeding by Mitochondrial Uncoupling and Increased Adipocyte Fatty Acid Oxidation

Mancuso, D. J., Sims, H. F., Yang, K., Kiebish, M. A., Su, X., Jenkins, C. M., Guan, S., Moon, S. H., Pietka, T., Nassir, F., et al. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.115766 · Cited by 89 · see citing works

Identification and Quantitation of Unsaturated Fatty Acid Isomers by Electrospray Ionization Tandem Mass Spectrometry: A Shotgun Lipidomics Approach

Yang, K., Zhao, Z., Gross, R. W., & Han, X. (2011) · Analytical Chemistry · doi:10.1021/ac2006119 · Cited by 89 · see citing works

Applications of mass spectrometry for cellular lipid analysis

Wang, C., Wang, M., & Han, X. (2015) · Molecular BioSystems · doi:10.1039/c4mb00586d · Cited by 87 · see citing works

A Role for Peroxisome Proliferator-activated Receptor γ Coactivator 1 (PGC-1) in the Regulation of Cardiac Mitochondrial Phospholipid Biosynthesis

Lai, L., Wang, M., Martin, O. J., Leone, T. C., Vega, R. B., Han, X., & Kelly, D. P. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.523654 · Cited by 86 · see citing works

Potential Adverse Effects of Prolonged Sevoflurane Exposure on Developing Monkey Brain: From Abnormal Lipid Metabolism to Neuronal Damage

Liu, F., Rainosek, S. W., Frisch‐Daiello, J. L., Patterson, T. A., Paule, M. G., Slikker, W., Wang, C., & Han, X. (2015) · Toxicological Sciences · doi:10.1093/toxsci/kfv150 · Cited by 85 · see citing works

A monophasic extraction strategy for the simultaneous lipidome analysis of polar and nonpolar retina lipids

Lydic, T. A., Busik, J. V., & Reid, G. E. (2014) · Journal of Lipid Research · doi:10.1194/jlr.d050302 · Cited by 85 · see citing works

Cytochrome b5 reductase and the control of lipid metabolism and healthspan

Martín‐Montalvo, A., Sun, Y., Díaz‐Ruiz, A., Ali, A., Gutierrez, V., Palacios, H. H., Curtis, J., Siendones, E., Ariza, J., Abulwerdi, G. A., et al. (2016) · npj Aging and Mechanisms of Disease · doi:10.1038/npjamd.2016.6 · Cited by 81 · see citing works

Recent advances of chromatography and mass spectrometry in lipidomics

Li, M., Zhou, Z., Nie, H., Bai, Y., & Liu, H. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-4327-y · Cited by 81 · see citing works

Enhanced defense against ferroptosis ameliorates cognitive impairment and reduces neurodegeneration in 5xFAD mice

Chen, L., Dar, N. J., Na, R., McLane, K. D., Yoo, K., Han, X., & Ran, Q. (2022) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2022.01.002 · Cited by 80 · see citing works

Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix

Cheng, H., Sun, G., Yang, K., Gross, R. W., & Han, X. (2010) · Journal of Lipid Research · doi:10.1194/jlr.d004077 · Cited by 79 · see citing works

Overview of Lipidomic Analysis of Triglyceride Molecular Species in Biological Lipid Extracts

Han, X., & Ye, H. (2021) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.0c07175 · Cited by 76 · see citing works

Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions

Boyd, J. T., Lococo, P. M., Furr, A. R., Bendele, M., Tram, M., Li, Q., Chang, F.-M., Colley, M. E., Samenuk, G. M., Arris, D., et al. (2021) · Nature Metabolism · doi:10.1038/s42255-021-00410-x · Cited by 71 · see citing works

Yeast Lipid Analysis and Quantification by Mass Spectrometry

Guan, X. L., Riezman, I., Wenk, M. R., & Riezman, H. (2010) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(10)70015-x · Cited by 72 · see citing works

Quantitative analysis of glycerophospholipids by LC–MS: Acquisition, data handling, and interpretation

Myers, D. S., Ivanova, P. T., Milne, S. B., & Brown, H. A. (2011) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2011.05.015 · Cited by 69 · see citing works

Novel molecular insights into the critical role of sulfatide in myelin maintenance/function

Palavicini, J. P., Wang, C., Chen, L., Ahmar, S., Higuera, J. D., Dupree, J. L., & Han, X. (2016) · Journal of Neurochemistry · doi:10.1111/jnc.13738 · Cited by 69 · see citing works

Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics

Kiebish, M. A., Bell, R., Yang, K., Phan, T., Zhao, Z., Ames, W. G., Seyfried, T. N., Gross, R. W., Chuang, J. H., & Han, X. (2010) · Journal of Lipid Research · doi:10.1194/jlr.m004796 · Cited by 68 · see citing works

Identification of Naturally Occurring Fatty Acids of the Myelin Sheath That Resolve Neuroinflammation

Ho, P. P.-K., Kanter, J. L., Johnson, A. M., Srinagesh, H. K., Chang, E., Purdy, T. M., van Haren, K. P., Wikoff, W. R., Kind, T., Khademi, M., et al. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3003831 · Cited by 67 · see citing works

Characterization and Quantification of Diacylglycerol Species in Biological Extracts after One-Step Derivatization: A Shotgun Lipidomics Approach

Wang, M., Hayakawa, J., Yang, K., & Han, X. (2014) · Analytical Chemistry · doi:10.1021/ac403798q · Cited by 67 · see citing works

Preparation of derivatives of fatty acids

Christie, W. W., & Han, X. (2012) · Elsevier eBooks · doi:10.1533/9780857097866.145 · Cited by 66 · see citing works

Shotgun Lipidomics Analysis of 4-Hydroxyalkenal Species Directly from Lipid Extracts after One-Step in Situ Derivatization

Wang, M., Fang, H., & Han, X. (2012) · Analytical Chemistry · doi:10.1021/ac300695p · Cited by 63 · see citing works

Oligomeric amyloid-beta induces MAPK-mediated activation of brain cytosolic and calcium-independent phospholipase A2 in a spatial-specific manner

Palavicini, J. P., Wang, C., Chen, L., HoSang, K. M., Wang, J., Tomiyama, T., Mori, H., & Han, X. (2017) · Acta Neuropathologica Communications · doi:10.1186/s40478-017-0460-6 · Cited by 61 · see citing works

Oxidative stress leads to reduction of plasmalogen serving as a novel biomarker for systemic lupus erythematosus

Hu, C., Zhou, J., Yang, S., Li, H., Wang, C., Fang, X., Fan, Y., Zhang, J., Han, X., & Wen, C. (2016) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2016.11.006 · Cited by 62 · see citing works

Apolipoprotein E regulates lipid metabolism and α-synuclein pathology in human iPSC-derived cerebral organoids

Zhao, J., Lü, W., Ren, Y., Fu, Y., Martens, Y. A., Shue, F., Davis, M. D., Wang, X., Chen, K., Li, F., et al. (2021) · Acta Neuropathologica · doi:10.1007/s00401-021-02361-9 · Cited by 60 · see citing works

De novo lipogenesis fuels adipocyte autophagosome and lysosome membrane dynamics

Rowland, L. A., Guilherme, A., Henriques, F. D. S., DiMarzio, C., Munroe, S., Wetoska, N., Kelly, M., Reddig, K. R., Hendricks, G. M., Pan, M., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-37016-8 · Cited by 60 · see citing works

Changes in the phospholipid molecular species in water-boiled salted duck during processing based on shotgun lipidomics

Li, C., Li, X., Huang, Q., Zhuo, Y., Xu, B., & Wang, Z. (2020) · Food Research International · doi:10.1016/j.foodres.2020.109064 · Cited by 56 · see citing works

ABCA7 deficiency causes neuronal dysregulation by altering mitochondrial lipid metabolism

Kawatani, K., Holm, M., Starling, S. C., Martens, Y. A., Zhao, J., Lü, W., Ren, Y., Li, Z., Jiang, P., Jiang, Y., et al. (2023) · Molecular Psychiatry · doi:10.1038/s41380-023-02372-w · Cited by 55 · see citing works

Lipidomics in tissues, cells and subcellular compartments

Horn, P. J., & Chapman, K. D. (2011) · The Plant Journal · doi:10.1111/j.1365-313x.2011.04868.x · Cited by 55 · see citing works

Targeted lipidomics – advances in profiling lysophosphocholine and platelet‐activating factor second messengers

Xu, H., Valenzuela, N., Fai, S., Figeys, D., & Bennett, S. A. L. (2013) · FEBS Journal · doi:10.1111/febs.12423 · Cited by 55 · see citing works

Absence of oligodendroglial glucosylceramide synthesis does not result in CNS myelin abnormalities or alter the dysmyelinating phenotype of CGT‐deficient mice

Saadat, L., Dupree, J. L., Kilkus, J., Han, X., Traka, M., Proia, R. L., Dawson, G., & Popko, B. (2010) · Glia · doi:10.1002/glia.20930 · Cited by 61 · see citing works

Comprehensive and Quantitative Analysis of Lysophospholipid Molecular Species Present in Obese Mouse Liver by Shotgun Lipidomics

Wang, C., Wang, M., & Han, X. (2015) · Analytical Chemistry · doi:10.1021/acs.analchem.5b00410 · Cited by 50 · see citing works

Quantitative Spatial Analysis of the Mouse Brain Lipidome by Pressurized Liquid Extraction Surface Analysis

Almeida, R., Berzina, Z., Arnspang, E. C., Baumgart, J., Vogt, J., Nitsch, R., & Ejsing, C. S. (2014) · Analytical Chemistry · doi:10.1021/ac503627z · Cited by 49 · see citing works

Early disruption of nerve mitochondrial and myelin lipid homeostasis in obesity-induced diabetes

Palavicini, J. P., Chen, J., Wang, C., Wang, J., Qin, C., Baeuerle, E., Wang, X., Woo, J. A., Kang, D. E., Musi, N., et al. (2020) · JCI Insight · doi:10.1172/jci.insight.137286 · Cited by 49 · see citing works

Oxidative stress-induced aberrant lipid metabolism is an important causal factor for dysfunction of immunocytes from patients with systemic lupus erythematosus

Hu, C., Zhang, J., Hong, S., Li, H., Lu, L., Xie, G., Luo, W., Du, Y., Xie, Z., Han, X., et al. (2020) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2020.12.006 · Cited by 48 · see citing works

Shotgun Lipidomics Revealed Altered Profiles of Serum Lipids in Systemic Lupus Erythematosus Closely Associated with Disease Activity

Lu, L., Hu, C., Zhao, Y., He, L., Zhou, J., Li, H., Du, Y., Wang, Y., Wen, C., Han, X., et al. (2018) · Biomolecules · doi:10.3390/biom8040105 · Cited by 48 · see citing works

Sensitive analysis of fatty acid esters of hydroxy fatty acids in biological lipid extracts by shotgun lipidomics after one-step derivatization

Hu, C., Wang, M., Duan, Q., & Han, X. (2020) · Analytica Chimica Acta · doi:10.1016/j.aca.2020.01.026 · Cited by 47 · see citing works

An RNAi therapeutic targeting hepatic DGAT2 in a genetically obese mouse model of nonalcoholic steatohepatitis

Yenilmez, B., Wetoska, N., Kelly, M., Echeverria, D., Min, K., Lifshitz, L., Alterman, J. F., Hassler, M. R., Hildebrand, S., DiMarzio, C., et al. (2022) · Molecular Therapy · doi:10.1016/j.ymthe.2021.11.007 · Cited by 46 · see citing works

Loss of transcriptional repression by BCL6 confers insulin sensitivity in the setting of obesity

Senagolage, M. D., Sommars, M. A., Ramachandran, K., Futtner, C. R., Omura, Y., Allred, A. L., Wang, J., Yang, C., Procissi, D., Evans, R. M., et al. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.11.074 · Cited by 44 · see citing works

Analytical challenges of shotgun lipidomics at different resolution of measurements

Wang, J., & Han, X. (2019) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2019.115697 · Cited by 43 · see citing works

Lipocalin 2 regulates mitochondrial phospholipidome remodeling, dynamics, and function in brown adipose tissue in male mice

Su, H., Guo, H., Qiu, X., Lin, T., Qin, C., Celio, G. J., Yong, P., Senders, M., Han, X., Bernlohr, D., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-42473-2 · Cited by 41 · see citing works

Simulation of triacylglycerol ion profiles: bioinformatics for interpretation of triacylglycerol biosynthesis

Han, R. H., Wang, M., Fang, X., & Han, X. (2013) · Journal of Lipid Research · doi:10.1194/jlr.m033837 · Cited by 42 · see citing works

Comprehensive and Quantitative Analysis of Polyphosphoinositide Species by Shotgun Lipidomics Revealed Their Alterations in db/db Mouse Brain

Wang, C., Palavicini, J. P., Wang, M., Chen, L., Yang, K., Crawford, P. A., & Han, X. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.6b02947 · Cited by 42 · see citing works

Phospholipid imbalance impairs autophagosome completion

Polyansky, A., Shatz, O., Fraiberg, M., Shimoni, E., Dadosh, T., Mari, M. C., Reggiori, F., Qin, C., Han, X., & Elazar, Z. (2022) · The EMBO Journal · doi:10.15252/embj.2022110771 · Cited by 40 · see citing works

Lipidomics Revealed Idiopathic Pulmonary Fibrosis-Induced Hepatic Lipid Disorders Corrected with Treatment of Baicalin in a Murine Model

Hu, C., Wang, Y., Fan, Y., Li, H., Wang, C., Zhang, J., Zhang, S.-J., Han, X., & Wen, C. (2015) · The AAPS Journal · doi:10.1208/s12248-014-9714-4 · Cited by 41 · see citing works

Strategy for Quantitative Analysis of Isomeric Bis(monoacylglycero)phosphate and Phosphatidylglycerol Species by Shotgun Lipidomics after One-Step Methylation

Wang, M., Palavicini, J. P., Cseresznye, A., & Han, X. (2017) · Analytical Chemistry · doi:10.1021/acs.analchem.7b02058 · Cited by 40 · see citing works

MondoA drives muscle lipid accumulation and insulin resistance

Ahn, B. Y., Wan, S., Jaiswal, N., Vega, R. B., Ayer, D. E., Titchenell, P. M., Han, X., Won, K., & Kelly, D. P. (2019) · JCI Insight · doi:10.1172/jci.insight.129119 · Cited by 40 · see citing works

On the future of “omics”: lipidomics

Griffiths, W. J., Ogundare, M., Williams, C. M., & Wang, Y. (2011) · Journal of Inherited Metabolic Disease · doi:10.1007/s10545-010-9274-4 · Cited by 38 · see citing works

Direct quantitation of psychosine from alkaline-treated lipid extracts with a semi-synthetic internal standard

Jiang, X., Yang, K., & Han, X. (2008) · Journal of Lipid Research · doi:10.1194/jlr.d800036-jlr200 · Cited by 37 · see citing works

Accurate mass searching of individual lipid species candidates from high‐resolution mass spectra for shotgun lipidomics

Wang, M., Huang, Y., & Han, X. (2014) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.7015 · Cited by 35 · see citing works

Paradoxical activation of transcription factor SREBP1c and de novo lipogenesis by hepatocyte-selective ATP-citrate lyase depletion in obese mice

Yenilmez, B., Kelly, M., Zhang, G., Wetoska, N., Ilkayeva, O., Min, K., Rowland, L. A., DiMarzio, C., He, W., Raymond, N., et al. (2022) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2022.102401 · Cited by 35 · see citing works

Acetyl-CoA carboxylase 1 is a suppressor of the adipocyte thermogenic program

Guilherme, A., Rowland, L. A., Wetoska, N., Tsagkaraki, E., Santos, K. B., Bedard, A. H., Henriques, F. D. S., Kelly, M., Munroe, S., Pedersen, D. J., et al. (2023) · Cell Reports · doi:10.1016/j.celrep.2023.112488 · Cited by 34 · see citing works

High-Throughput Characterization of Small and Large Molecules Using Only a Matrix and the Vacuum of a Mass Spectrometer

Woodall, D. W., Wang, B., Inutan, E. D., Narayan, S. B., & Trimpin, S. (2015) · Analytical Chemistry · doi:10.1021/ac504475x · Cited by 33 · see citing works

Multidimensional Approaches in LC and MS for Phospholipid Bioanalysis

Guo, X., & Lankmayr, E. P. (2010) · Bioanalysis · doi:10.4155/bio.10.52 · Cited by 33 · see citing works

Current developments in high‐throughput analysis for microalgae cellular contents

Lee, T.-H., Chang, J., & Wang, H.-Y. A. (2013) · Biotechnology Journal · doi:10.1002/biot.201200391 · Cited by 33 · see citing works

Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase

Takahashi, H., Bhagwagar, S. Z., Nies, S. H., Ye, H., Han, X., Chiasseu, M., Wang, G., Mackenzie, I. R., & Strittmatter, S. M. (2024) · Nature Communications · doi:10.1038/s41467-024-45692-3 · Cited by 32 · see citing works

Myeloid-Specific Deficiency of Long-Chain Acyl CoA Synthetase 4 Reduces Inflammation by Remodeling Phospholipids and Reducing Production of Arachidonic Acid–Derived Proinflammatory Lipid Mediators

Reeves, A. R., Sansbury, B. E., Pan, M., Han, X., Spite, M. R., & Greenberg, A. S. (2021) · The Journal of Immunology · doi:10.4049/jimmunol.2100393 · Cited by 30 · see citing works

STAT3 activation of SCAP-SREBP-1 signaling upregulates fatty acid synthesis to promote tumor growth

Fan, Y., Zhang, R., Wang, C., Pan, M., Geng, F., Zhong, Y., Su, H., Kou, Y., Mo, X. M., Lefai, É., et al. (2024) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2024.107351 · Cited by 27 · see citing works

Mitochondrial ACSS1-K635 acetylation knock-in mice exhibit altered metabolism, cell senescence, and nonalcoholic fatty liver disease

Xu, G., Quan, S., Schell, J. R., Gao, Y., Varmazyad, M., Sreenivas, P., Cruz, D., Jiang, H., Pan, M., Han, X., et al. (2024) · Science Advances · doi:10.1126/sciadv.adj5942 · Cited by 27 · see citing works

Reversible deficits in apical transporter trafficking associated with deficiency in diacylglycerol acyltransferase

Schlegel, C. E., Lapierre, L. A., Weis, V. G., Williams, J. A., Kaji, I., Pinzon‐Guzman, C., Prasad, N., Boone, B. E., Jones, A., Correa, H., et al. (2018) · Traffic · doi:10.1111/tra.12608 · Cited by 27 · see citing works

Lipids: their structures and occurrence

Christie, W. W., & Han, X. (2012) · Elsevier eBooks · doi:10.1533/9780857097866.3 · Cited by 26 · see citing works

Synthesis and Biological Evaluation of Antimetastatic Agents Predicated upon Dihydromotuporamine C and Its Carbocyclic Derivatives

Muth, A., Pandey, V., Kaur, N., Wason, M. S., Baker, C. H., Han, X., Johnson, T. R., Altomare, D. A., & Phanstiel, O. (2014) · Journal of Medicinal Chemistry · doi:10.1021/jm401906v · Cited by 25 · see citing works

Influence of Salt Content Used for Dry-Curing on Lipidomic Profiles during the Processing of Water-Boiled Salted Duck

Li, C., Li, X., Huang, Q., Zhou, Y. F., Xu, B., & Wang, Z. (2020) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.0c01513 · Cited by 25 · see citing works

Lipidomics-Assisted GWAS (lGWAS) Approach for Improving High-Temperature Stress Tolerance of Crops

Pranneshraj, V., Sangha, M. K., Djalović, I. G., Miladinović, J., & Djanaguiraman, M. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23169389 · Cited by 23 · see citing works

Multidimensional mass spectrometry-based shotgun lipidomics analysis of vinyl ether diglycerides

Yang, K., Jenkins, C. M., Dilthey, B. G., & Gross, R. W. (2015) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-015-8640-3 · Cited by 23 · see citing works

Lipidomics reveals a systemic energy deficient state that precedes neurotoxicity in neonatal monkeys after sevoflurane exposure

Wang, C., Liu, F., Frisch‐Daiello, J. L., Martin, S. E., Patterson, T. A., Gu, Q., Liu, S., Paule, M. G., Hanig, J. P., Slikker, W., et al. (2017) · Analytica Chimica Acta · doi:10.1016/j.aca.2017.11.052 · Cited by 23 · see citing works

Docosahexaenoic Acid (DHA) Supplementation Alters Phospholipid Species and Lipid Peroxidation Products in Adult Mouse Brain, Heart, and Plasma

Sun, G. Y., Appenteng, M. K., Li, R., Woo, T., Yang, B., Qin, C., Pan, M., Cieślik, M., Cui, J., Fritsche, K. L., et al. (2020) · NeuroMolecular Medicine · doi:10.1007/s12017-020-08616-0 · Cited by 22 · see citing works

Lipidomic analysis reveals significant lipogenesis and accumulation of lipotoxic components in ob/ob mouse organs

Hayakawa, J., Wang, M., Wang, C., Han, R. H., Jiang, Z. Y., & Han, X. (2017) · Prostaglandins Leukotrienes and Essential Fatty Acids · doi:10.1016/j.plefa.2017.01.002 · Cited by 21 · see citing works

Sulfatide Deficiency, an Early Alzheimer’s Lipidomic Signature, Causes Brain Ventricular Enlargement in the Absence of Classical Neuropathological Hallmarks

Ding, L., Palavicini, J. P., Pan, M., Qiu, S., Wang, H., Shen, Q., Dupree, J. L., & Han, X. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms24010233 · Cited by 19 · see citing works

A Practical Approach for Determination of Mass Spectral Baselines

Yang, K., Fang, X., Gross, R. W., & Han, X. (2011) · Journal of the American Society for Mass Spectrometry · doi:10.1007/s13361-011-0229-2 · Cited by 20 · see citing works

Gas chromatographic analysis of fatty acid derivatives

Christie, W. W., & Han, X. (2012) · Elsevier eBooks · doi:10.1533/9780857097866.159 · Cited by 20 · see citing works

Alterations in Mouse Brain Lipidome after Disruption of CST Gene: A Lipidomics Study

Wang, C., Wang, M., Zhou, Y., Dupree, J. L., & Han, X. (2014) · Molecular Neurobiology · doi:10.1007/s12035-013-8626-0 · Cited by 20 · see citing works

Shotgun lipidomics: multidimensional MS analysis of cellular lipidomes

Han, X., & Gross, R. W. (2005) · Expert Review of Proteomics · doi:10.1586/14789450.2.2.253 · Cited by 278 · see citing works

Factors influencing the electrospray intrasource separation and selective ionization of glycerophospholipids

Han, X., Yang, K., Yang, J., Fikes, K. N., Cheng, H., & Gross, R. W. (2006) · Journal of the American Society for Mass Spectrometry · doi:10.1016/j.jasms.2005.11.003 · Cited by 127 · see citing works

Shotgun lipidomics: Electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples

Han, X., & Gross, R. W. (2004) · Mass Spectrometry Reviews · doi:10.1002/mas.20023 · Cited by 1,111 · see citing works

Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry

Özbalci, C., Sachsenheimer, T., & Brügger, B. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-487-6_1 · Cited by 86 · see citing works

Toward fingerprinting cellular lipidomes directly from biological samples by two-dimensional electrospray ionization mass spectrometry

Han, X., Yang, J., Cheng, H., Ye, H., & Gross, R. W. (2004) · Analytical Biochemistry · doi:10.1016/j.ab.2004.04.004 · Cited by 219 · see citing works