The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Cantilever-Based Optical Deflection Assay for Discrimination of DNA Single-Nucleotide Mismatches
Abstract
Characterization of single-nucleotide polymorphisms is a major focus of current genomics research. We demonstrate the discrimination of DNA mismatches using an elegantly simple microcantilever-based optical deflection assay, without the need for external labeling. Gold-coated silicon AFM cantilevers were functionalized with thiolated 20- or 25-mer probe DNA oligonucleotides and exposed to target oligonucleotides of varying sequence in static and flow conditions. Hybridization of 10-mer complementary target oligonucleotides resulted in net positive deflection, while hybridization with targets containing one or two internal mismatches resulted in net negative deflection. Mismatched targets produced a stable and measurable signal when only a four-base pair stretch was complementary to the probe sequence. This technique is readily adaptable to a high-throughput array format and provides a distinct positive/negative signal for easy interpretation of oligonucleotide hybridization.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Fundamentals of Microfabrication
Madou, M. J. (2018) · CRC Press eBooks · doi:10.1201/9781482274004 · Cited by 2,020
Translating Biomolecular Recognition into Nanomechanics
Fritz, J., Baller, M. K., Lang, H. P., Rothuizen, H., Vettiger, P., Meyer, E., -J. Güntherodt, H., Gerber, C., & Gimzewski, J. K. (2000) · Science · doi:10.1126/science.288.5464.316 · Cited by 1,646
Ionic effects on the elasticity of single DNA molecules
Baumann, C. G., Smith, S. B., Bloomfield, V. A., & Bustamante, C. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.12.6185 · Cited by 1,001
Fischer, S. G., & Lerman, L. S. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.6.1579 · Cited by 892
Ramsay, G. (1998) · Nature Biotechnology · doi:10.1038/nbt0198-40 · Cited by 781
(1915) · Journal of Education · doi:10.1177/002205741508200834 · Cited by 616
Peterlinz, K. A., Georgiadis, R. M., Herne, T. M., & Tarlov, M. J. (1997) · Journal of the American Chemical Society · doi:10.1021/ja964326c · Cited by 477
On the Nature of Water Bound to a Solid Acid Catalyst
Smith, L., Cheetham, A. K., Morris, R. E., Marchese, L., Thomas, J. M., Wright, P. A., & Chen, J. (1996) · Science · doi:10.1126/science.271.5250.799 · Cited by 265
Multiplexed single nucleotide polymorphism genotyping by oligonucleotide ligation and flow cytometry
Iannone, M. A., Taylor, J. D., Chen, J., Li, M.-S., Rivers, P., Slentz‐Kesler, K., & Weiner, M. P. (2000) · Cytometry · doi:10.1002/(sici)1097-0320(20000201)39:2<131::aid-cyto6>3.0.co;2-u · Cited by 217
DNA variation and the future of human genetics
Schafer, A. J., & Hawkins, J. R. (1998) · Nature Biotechnology · doi:10.1038/nbt0198-33 · Cited by 208
Cystic fibrosis mutation detection by hybridization to light-generated DNA probe arrays
Cronin, M. T., Fucini, R. V., Kim, S. M., Masino, R. S., Wespi, R. M., & Miyada, C. G. (1996) · Human Mutation · doi:10.1002/(sici)1098-1004(1996)7:3<244::aid-humu9>3.0.co;2-a · Cited by 171
Direct haplotyping of kilobase-size DNA using carbon nanotube probes
Woolley, A. T., Guillemette, C., Li Cheung, C., Housman, D. E., & Lieber, C. M. (2000) · Nature Biotechnology · doi:10.1038/77760 · Cited by 168
Cited by
Cancer nanotechnology: opportunities and challenges
Ferrari, M. (2005) · Nature Reviews Cancer · doi:10.1038/nrc1566 · Cited by 4,558
Cantilever transducers as a platform for chemical and biological sensors
Lavrik, N. V., Sepaniak, M. J., & Datskos, P. G. (2004) · Review of Scientific Instruments · doi:10.1063/1.1763252 · Cited by 1,117
BioMEMS: state-of-the-art in detection, opportunities and prospects
Bashir, R. (2004) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2004.03.002 · Cited by 462
Lipid‐based nanotherapeutics for siRNA delivery
Schroeder, A., Levins, C. G., Cortez, C., Langer, R., & Anderson, D. G. (2010) · Journal of Internal Medicine · doi:10.1111/j.1365-2796.2009.02189.x · Cited by 447
Ziegler, C. (2004) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-004-2694-y · Cited by 414
Metadata
- Authors
- Hansen, K. M., Ji, H.-F., Wu, G., Datar, R., Cote, R., Majumdar, A., & Thundat, T.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-03-02
- Volume
- 73
- Issue
- 7
- Pages
- 1567-1571
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2038637564
- Cited by
- 337
- References
- 12
- FWCI
- 6.62
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
