The DOI matches its Crossref record.
Cell Lysis and Protein Extraction in a Microfluidic Device with Detection by a Fluorogenic Enzyme Assay
Abstract
A critical requirement for achieving a micro total analytical system for the analysis of cells and their constituent proteins is to integrate the lysis and fractionation steps on-chip. Here, an experimental microfluidic system integrating the lysis of bacterial cells and the extraction of a large intracellular enzyme, beta-galactosidase, is demonstrated. The beta-galactosidase is detected and quantified using a fluorogenic enzyme assay and a numerical model. While the focus is on the lysis of typical gram-negative bacterial cells (E. coli), the techniques described here could, in principle, be applied to a variety of different cell types.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Au, A. K., Huynh, W., Horowitz, L. F., & Folch, A. (2016) · Angewandte Chemie International Edition · doi:10.1002/anie.201504382 · Cited by 788
Microfluidic Device for Single-Cell Analysis
Wheeler, A. R., Throndset, W. R., Whelan, R. J., Leach, A. M., Zare, R. N., Liao, Y. H., Farrell, K., Manger, I. D., & Daridon, A. (2003) · Analytical Chemistry · doi:10.1021/ac0340758 · Cited by 600
Zheng, S., Lin, H., Liu, J.-Q., Balic, M., Datar, R., Cote, R. J., & Tai, Y.-C. (2007) · Journal of Chromatography A · doi:10.1016/j.chroma.2007.05.064 · Cited by 599
Biomolecular gradients in cell culture systems
Keenan, T. M., & Folch, A. (2008) · Lab Chip · doi:10.1039/b711887b · Cited by 596
Lab-on-a-chip for drug development
Weigl, B. H., Bardell, R. L., & Cabrera, C. R. (2003) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(02)00223-5 · Cited by 481
Metadata
- Authors
- Schilling, E. A., Kamholz, A. E., & Yager, P.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-03-08
- Volume
- 74
- Issue
- 8
- Pages
- 1798-1804
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2007363842
- Cited by
- 299
- References
- 0
- FWCI
- 21.56
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Cell Lysis and Protein Extraction in a Microfluidic Device with Detection by a Fluorogenic Enzyme Assay. https://app.cochat.ai/papers/10.1021/ac015640e
- The paper
- Schilling, E. A., Kamholz, A. E., & Yager, P. (2002). Cell Lysis and Protein Extraction in a Microfluidic Device with Detection by a Fluorogenic Enzyme Assay. Analytical Chemistry. https://doi.org/10.1021/ac015640e
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
