Cited by 596Microfluidic and Capillary Electrophoresis ApplicationsMicrofluidic and Bio-sensing TechnologiesInnovative Microfluidic and Catalytic Techniques Innovation

Biomolecular gradients in cell culture systems

Open access
Keenan, Thomas M., Folch, Albert·2008·Lab Chip

Abstract

Biomolecule gradients have been shown to play roles in a wide range of biological processes including development, inflammation, wound healing, and cancer metastasis. Elucidation of these phenomena requires the ability to expose cells to biomolecule gradients that are quantifiable, controllable, and mimic those that are present in vivo. Here we review the major biological phenomena in which biomolecule gradients are employed, traditional in vitro gradient-generating methods developed over the past 50 years, and new microfluidic devices for generating gradients. Microfluidic gradient generators offer greater levels of precision, quantitation, and spatiotemporal gradient control than traditional methods, and may greatly enhance our understanding of many biological phenomena. For each method, we outline the salient features, capabilities, and applications.

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Crossref · 10.1039/b711887b

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123 of 123 references verified0 citation errors · 0 not checkable

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  • Bioprinting technology is able to create a biomimetic microenvironment for the heterogeneous distribution of biologically relevant proteins and growth factors, which are important to control tumor cell signaling, proliferation, and migration.

    “In addition, bioprinting technology is able to create a biomimetic microenvironment for the heterogeneous distribution of biologically relevant proteins and growth factors, which are important to control tumor cell signaling, proliferation, and migration156,157.”

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Authors
Keenan, T. M., & Folch, A.
Affiliations
University of Washington (US)
Corresponding author
Folch, Albert
Venue
Lab Chip
Published
2008
Volume
8
Issue
1
Pages
34-57
ISSN
  • 1473-0189 (ISSN-L)
  • 1473-0197
OpenAlex
W2082064020
Cited by
596
References
123
Citations by year
2026: 12 · 2025: 18 · 2024: 17 · 2023: 22 · 2022: 17 · 2021: 22 · 2020: 26 · 2019: 28 · 2018: 36 · 2017: 24 · 2016: 38 · 2015: 43 · 2014: 37 · 2013: 49 · 2012: 46
FWCI
3.06
Type
journal article
Language
en
Open access
Locations
  • Lab on a Chip (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute of Neurological Disorders and Stroke (R01NS064387)
  • National Institute of Biomedical Imaging and Bioengineering (R33EB003307)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
  • Physical Sciences › Engineering › Biomedical Engineering › Innovative Microfluidic and Catalytic Techniques Innovation
Keywords
gradient generation, microfluidic gradient generator, cell culture, signal transduction, metastasis, wound healing, inflammation, development (biology), spatiotemporal control
MeSH terms
Animals, Biological Factors, Cell Physiological Phenomena, Substrate Specificity, Time Factors, Cell Culture Techniques, Microfluidic Analytical Techniques

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