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Microfluidic Device for Single-Cell Analysis
Abstract
We have developed a novel microfluidic device constructed from poly(dimethylsiloxane) using multilayer soft lithography technology for the analysis of single cells. The microfluidic network enables the passive and gentle separation of a single cell from the bulk cell suspension, and integrated valves and pumps enable the precise delivery of nanoliter volumes of reagents to that cell. Various applications are demonstrated, including cell viability assays, ionophore-mediated intracellular Ca2+ flux measurements, and multistep receptor-mediated Ca2+ measurements. These assays, and others, are achieved with significant improvements in reagent consumption, analysis time, and temporal resolution over macroscale alternatives.
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Citations
Cited by
The origins and the future of microfluidics
Whitesides, G. M. (2006) · Nature · doi:10.1038/nature05058 · Cited by 9,687
Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications
Mark, D., Haeberle, S., Roth, G., von Stetten, F., & Zengerle, R. (2010) · Chemical Society Reviews · doi:10.1039/b820557b · Cited by 1,647
Buettner, F., Natarajan, K. N., Casale, F. P., Proserpio, V., Scialdone, A., Theis, F. J., Teichmann, S. A., Marioni, J. C., & Stegle, O. (2015) · Nature Biotechnology · doi:10.1038/nbt.3102 · Cited by 1,136
Microfluidics for in vivo imaging of neuronal and behavioral activity in Caenorhabditis elegans
Chronis, N., Zimmer, M., & Bargmann, C. I. (2007) · Nature Methods · doi:10.1038/nmeth1075 · Cited by 594
Localized Surface Plasmon Resonance Biosensors
Zhao, J., Zhang, X., Yonzon, C. R., Haes, A. J., & Van Duyne, R. P. (2006) · Nanomedicine · doi:10.2217/17435889.1.2.219 · Cited by 508
Metadata
- Authors
- Wheeler, A. R., Throndset, W. R., Whelan, R. J., Leach, A. M., Zare, R. N., Liao, Y. H., Farrell, K., Manger, I. D., & Daridon, A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-05-14
- Volume
- 75
- Issue
- 14
- Pages
- 3581-3586
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2036758684
- Cited by
- 600
- References
- 0
- FWCI
- 27.16
- Type
- journal article
- Language
- en
- Open access
- closed
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- Wheeler, A. R., Throndset, W. R., Whelan, R. J., Leach, A. M., Zare, R. N., Liao, Y. H., Farrell, K., Manger, I. D., & Daridon, A. (2003). Microfluidic Device for Single-Cell Analysis. Analytical Chemistry. https://doi.org/10.1021/ac0340758
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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