The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Measurement of the Oxygen Isotopic Composition of Nitrate in Seawater and Freshwater Using the Denitrifier Method
Abstract
We report a novel method for measurement of the oxygen isotopic composition (18O/16O) of nitrate (NO3-) from both seawater and freshwater. The denitrifier method, based on the isotope ratio analysis of nitrous oxide generated from sample nitrate by cultured denitrifying bacteria, has been described elsewhere for its use in nitrogen isotope ratio (15N/14N) analysis of nitrate. (1) Here, we address the additional issues associated with 18O/16O analysis of nitrate by this approach, which include (1) the oxygen isotopic difference between the nitrate sample and the N20 analyte due to isotopic fractionation associated with the loss of oxygen atoms from nitrate and (2) the exchange of oxygen atoms with water during the conversion of nitrate to N2O. Experiments with 18O-labeled water indicate that water exchange contributes less than 10%, and frequently less than 3%, of the oxygen atoms in the N20 product for Pseudomonas aureofaciens. In addition, both oxygen isotope fractionation and oxygen atom exchange are consistent within a given batch of analyses. The analysis of appropriate isotopic reference materials can thus be used to correct the measured 18O/16O ratios of samples for both effects. This is the first method tested for 18O/16O analysis of nitrate in seawater. Benefits of this method, relative to published freshwater methods, include higher sensitivity (tested down to 10 nmol and 1 microM NO3-), lack of interference by other solutes, and ease of sample preparation.
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Citations
Cites
The δ18O of dissolved O2 in seawater: A unique tracer of circulation and respiration in the deep sea
Bender, M. L. (1990) · Journal of Geophysical Research: Oceans · doi:10.1029/jc095ic12p22243 · Cited by 121
Cited by
METHODS FOR MEASURING DENITRIFICATION: DIVERSE APPROACHES TO A DIFFICULT PROBLEM
Groffman, P. M., Altabet, M. A., Böhlke, J. K., Butterbach-Bahl, K., David, M. B., Firestone, M. K., Giblin, A. E., Kana, T. M., Nielsen, L. P., & Voytek, M. A. (2006) · Ecological Applications · doi:10.1890/1051-0761(2006)016[2091:mfmdda]2.0.co;2 · Cited by 936
Self-citationMcIlvin, M. R., & Altabet, M. A. (2005) · Analytical Chemistry · doi:10.1021/ac050528s · Cited by 718
Saggar, S., Jha, N., Deslippe, J., Bolan, N. S., Luo, J., Giltrap, D. L., Kim, D.-G., Zaman, M., & Tillman, R. W. (2013) · Science of The Total Environment · doi:10.1016/j.scitotenv.2012.11.050 · Cited by 543
Sutka, R. L., Ostrom, N. E., Ostrom, P. H., Breznak, J. A., Gandhi, H., Pitt, A. J., & Li, F. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.72.1.638-644.2006 · Cited by 531
Böhlke, J. K., Mroczkowski, S. J., & Coplen, T. B. (2003) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.1123 · Cited by 465
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Metadata
- Authors
- Casciotti, K. L., Sigman, D. M., Hastings, M. G., Böhlke, J. K., & Hilkert, A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-09-07
- Volume
- 74
- Issue
- 19
- Pages
- 4905-4912
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2025254507
- Cited by
- 1,595
- References
- 1
- FWCI
- 18.35
- Type
- journal article
- Language
- en
- Open access
- closed
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