Detection of Malaria Parasites in Blood by Laser Desorption Mass Spectrometry
Abstract
A novel method for the in vitro detection of the protozoan Plasmodium, the causative agent of malaria, has been developed. It comprises a protocol for cleanup of whole blood samples, followed by direct ultraviolet laser desorption (LD) time-of-flight mass spectrometry. Intense ion signals are observed from intact ferriprotoporphyrin IX (heme), sequestered by malaria parasites during their growth in human red blood cells. The LD mass spectrum of the heme is structure-specific, and the signal intensities are correlated with the sample parasitemia (number of parasites per unit volume of blood). Parasitemia levels on the order of 10 parasites/microL blood can be unambiguously detected by this method. Consideration of laser beam parameters (spot size, rastering across the sample surface) and actual sample consumption suggests that the detection limits can be further improved by at least an order of magnitude. The influence of experimental factors, such as desorbed ion polarity, laser exposure and fluence, sample size, and parasite growth stage, on the threshold for parasite detection is also addressed.
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Hemozoin can be detected by polarized light, by fluorescent properties allowing quantification in tissue, and by laser desorption mass spectrometry.
“Hemozoin can be detected by polarized light [48], by the fluorescent properties of hemozoin which allows quantification in tissue [49], and by laser desorption mass spectrometry (LDMS) that identifies distinct spectral features [50].”
From Diagnosing malaria in pregnancy: An update (2012) · cites this paper as [50]
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Diagnosing malaria in pregnancy: An update
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Self-citation
- Authors
- Demirev, P. A., Feldman, A. B., Kongkasuriyachai, D., Scholl, P., Sullivan, & Kumar, N.
- Affiliations
- Johns Hopkins University (US)
- Corresponding author
- Demirev, P. A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-06-04
- Volume
- 74
- Issue
- 14
- Pages
- 3262-3266
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1998301395
- Cited by
- 82
- References
- 22
- Citations by year
- 2024: 1 · 2023: 3 · 2019: 3 · 2018: 5 · 2017: 4 · 2016: 6 · 2015: 4 · 2014: 2 · 2013: 4 · 2012: 7
- FWCI
- 3.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Center for Research Resources (M01RR000722, RR00722)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Keywords
- malaria, Plasmodium, parasite detection, laser desorption mass spectrometry, time-of-flight mass spectrometry, ferriprotoporphyrin IX, heme, parasitemia, detection limit, laser fluence
- MeSH terms
- Animals, Erythrocytes, Hemin, Humans, Plasmodium falciparum, Molecular Structure, Malaria, Falciparum, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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