DETECTION OF PLASMODIUM FALCIPARUM IN PREGNANCY BY LASER DESORPTION MASS SPECTROMETRY
Abstract
Detection of Plasmodium falciparum malaria during pregnancy is complicated by sequestration of parasites in the placenta, which reduces peripheral blood microscopic detection. Laser desorption mass spectrometry (LDMS) has previously demonstrated sensitive detection of hemozoin from P. falciparum blood cultures and the ability to track parasitemia in a Plasmodium yoelii malaria mouse model. Here we use a simple, dilution in water, blood sample preparation protocol for LDMS detection of malaria in 45 asymptomatic, pregnant Zambian women. We compare LDMS to microscopy and polymerase chain reaction (PCR) analysis. All women were microscopy negative. LDMS detected P. falciparum hemozoin in 15 out of 45 women, while PCR results were positive in 25 women. Compared with PCR, which analyzed 20–30 μL of blood, the sensitivity of LDMS, which analyzed < 1 μL of blood, was 52%, with a specificity of 92%. LDMS is a potentially rapid and more sensitive alternate diagnostic method than microscopy.
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LDMS does not distinguish malaria species, and macrophages containing hemozoin can yield positive results, complicating differentiation of current versus past infection.
“However, LDMS does not distinguish malaria species, and macrophages containing hemozoin can yield positive results, which complicates the differentiation of current versus past infection [52].”
From Diagnosing malaria in pregnancy: An update (2012) · cites this paper as [52]
LDMS detects parasites in the range of 100-1000/μl blood in samples collected from pregnant women, similar to microscopy.
“LDMS detects parasites in the range of 100–1000/μl blood in samples collected from pregnant women, similar to microscopy.”
From Diagnosing malaria in pregnancy: An update (2012) · cites this paper as [52]
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- Authors
- NYUNT, M., PISCIOTTA, J., FELDMAN, A. B., THUMA, P., SCHOLL, P. F., DEMIREV, P. A., LIN, J. S., SHI, L., KUMAR, N., & SULLIVAN, D. J.
- Affiliations
- Johns Hopkins University (US)
- Corresponding author
- SULLIVAN, DAVID J.
- ORCID
- Venue
- The American Journal of Tropical Medicine and Hygiene
- Published
- 2005-09
- Volume
- 73
- Issue
- 3
- Pages
- 485-490
- ISSN
- 0002-9637 (ISSN-L)
- OpenAlex
- W2125170939
- Cited by
- 43
- References
- 20
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 4 · 2015: 4 · 2014: 2 · 2013: 3 · 2012: 4
- FWCI
- 3.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Tropical Medicine and Hygiene (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (R01 AI45774, R01AI045774)
- National Center for Research Resources (RR 0052)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Keywords
- Plasmodium falciparum, malaria in pregnancy, laser desorption mass spectrometry, asymptomatic malaria, placental sequestration, malaria diagnosis, sensitivity and specificity, PCR comparison, blood sample preparation
- MeSH terms
- Adult, Animals, Antimalarials, Chloroquine, Drug Combinations, Drug Resistance, Female, Genotype, Humans, Plasmodium falciparum, Pregnancy, Pyrimethamine, Sulfadoxine, Pregnancy Complications, Parasitic, Malaria, Falciparum, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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