The DOI matches its Crossref record.
Fast-Response Proteomics by Accelerated In-Gel Digestion of Proteins
Open accessAbstract
Kinetics of in-gel digestion of proteins by modified and native trypsins was studied by MALDI TOF mass spectrometry using 18O-labeled peptides as internal standards. The effect of the temperature, enzyme concentration, digestion time, and surface area of gel pieces on the yield of digestion products was characterized. Based on the kinetic data, we developed a protocol that enabled the identification of gel-separated proteins with 30-min digestion time without compromising the peptide yield and the sensitivity compared to conventional protocols that typically rely upon overnight enzymatic cleavage. The accelerated digestion protocol was tested in identification of more than 120 proteins from budding and fission yeasts at the subpicomole level.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
In-gel digestion for mass spectrometric characterization of proteins and proteomes
Shevchenko, A., Tomas, H., Havli, J., Olsen, J. V., & Mann, M. (2006) · Nature Protocols · doi:10.1038/nprot.2006.468 · Cited by 4,942
Self-citationSuspension trapping (STrap) sample preparation method for bottom‐up proteomics analysis
Zougman, A., Selby, P. J., & Banks, R. E. (2014) · PROTEOMICS · doi:10.1002/pmic.201300553 · Cited by 429
Chung, K. M., Liszewski, M. K., Nybakken, G., Davis, A. E., Townsend, R. R., Fremont, D. H., Atkinson, J. P., & Diamond, M. S. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0605668103 · Cited by 238
Martínez‐Gomariz, M., Perumal, P., Mekala, S., Nombela, C., Chaffin, W. L., & Gil, C. (2009) · PROTEOMICS · doi:10.1002/pmic.200700594 · Cited by 101
Biomarkers for Prediction of Bovine Respiratory Disease Outcome
Aich, P., Babiuk, L. A., Potter, A. A., & Griebel, P. (2009) · OMICS: A Journal of Integrative Biology · doi:10.1089/omi.2009.0012 · Cited by 46
Metadata
- Authors
- Havliš, J., Thomas, H., Šebela, M., & Shevchenko, A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-02-07
- Volume
- 75
- Issue
- 6
- Pages
- 1300-1306
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1987656271
- Cited by
- 373
- References
- 0
- FWCI
- 7.87
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
