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Solving the “World-to-Chip” Interface Problem with a Microfluidic Matrix
Abstract
We report an effective solution to the macroscopic/microfluidic interface issue and demonstrate how microfluidics can achieve impressive economies of scale in reducing the complexity of pipetting operations. Using an N x N microfluidic matrix with N = 20, we performed N2 = 400 distinct PCR reactions with only 2N + 1 = 41 pipetting steps, compared with the 3N2 = 1200 steps required with conventional fluid handling. Each vertex of the matrix has a 3-nL reactor, and a single 2-microL aliquot of polymerase is amortized over all 400 independent reactions, thus dramatically reducing sample overhead and minimizing reagent consumption. Beyond PCR, the matrix chip provides a general method to perform chemical and biological experiments with precious reagents in a highly automated fashion.
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Cited by
Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications
Mark, D., Haeberle, S., Roth, G., von Stetten, F., & Zengerle, R. (2010) · Chemical Society Reviews · doi:10.1039/b820557b · Cited by 1,647
Buettner, F., Natarajan, K. N., Casale, F. P., Proserpio, V., Scialdone, A., Theis, F. J., Teichmann, S. A., Marioni, J. C., & Stegle, O. (2015) · Nature Biotechnology · doi:10.1038/nbt.3102 · Cited by 1,136
Low-Cost Printing of Poly(dimethylsiloxane) Barriers To Define Microchannels in Paper
Bruzewicz, D. A., Reches, M., & Whitesides, G. M. (2008) · Analytical Chemistry · doi:10.1021/ac702605a · Cited by 616
High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array
Spurgeon, S. L., Jones, R. C., & Ramakrishnan, R. (2008) · PLoS ONE · doi:10.1371/journal.pone.0001662 · Cited by 386
Active droplet sorting in microfluidics: a review
Xi, H.-D., Zheng, H., Guo, W., Gañán-Calvo, A. M., Ai, Y., Tsao, C.-W., Zhou, J., Li, W., Huang, Y., Nguyen, N.-T., et al. (2017) · Lab on a Chip · doi:10.1039/c6lc01435f · Cited by 369
Shen, F., Davydova, E. K., Du, W., Kreutz, J. E., Piepenburg, O., & Ismagilov, R. F. (2011) · Analytical Chemistry · doi:10.1021/ac200247e · Cited by 240
Hahn, S., Jackson, L. G., Kolla, V., Mahyuddin, A. P., & Choolani, M. (2009) · Expert Review of Molecular Diagnostics · doi:10.1586/erm.09.43 · Cited by 32
Metadata
- Authors
- Liu, J., Hansen, C., & Quake, S. R.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-08-16
- Volume
- 75
- Issue
- 18
- Pages
- 4718-4723
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2074764083
- Cited by
- 276
- References
- 0
- FWCI
- 13.83
- Type
- journal article
- Language
- en
- Open access
- closed
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
